HybriDetect - Universal Lateral Flow Assay Kit
240.00 €
(Price excludes VAT and shipping costs)
100 tests
The HybriDetect is a simple and quick tool to develop your own rapid test. Various molecules can be detected: Proteins, Antibodies, Genetic amplicons. The HybriDetect Kit contains the buffer MHCB 1 but is also compatible with our buffers MHCBA 1 and MHCBB 1.
Please note, that you may have to pay country-specific taxes and duties.
Overview
Name of Product
HybriDetect - Universal Lateral Flow Assay Kit
Catalog Number
MGHD 1
Short Info
The HybriDetect is a simple and quick tool to develop your own rapid test. Various molecules can be detected: Proteins, Antibodies, Genetic amplicons. The HybriDetect Kit contains the buffer MHCB 1 but is also compatible with our buffers MHCBA 1 and MHCBB 1.
Please note, that you may have to pay country-specific taxes and duties.
Method/Platform
Lateral flow, sandwich or competetive
Range
5 pg DNA, eqivalent to agarose gel electrophoresis
Test Principle
HybriDetect is a ready-to-use, universal test strip (dipstick) based on lateral flow technology using gold particles. The dipstick can be used to develop qualitative or quantitative rapid test systems for a wide range of analytes such as antibodies, gene amplification products or proteins . The results can be interpreted qualitative or quantitative.
Protocol
Brief Instructions
Storage
2-8°C
Components
HybriDetect dipsticks, HybriDetect assay buffer
References
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Development and evaluation of a novel visual and rapid detection assay for toxigenic Fusarium graminearum in maize based on recombinase polymerase amplification and lateral flow analysis (2022)
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Development and evaluation of an isothermal recombinase polymerase amplification–lateral flow assay for rapid detection of strawberry vein banding virus in the field (2022)
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Development and utilization of a visual loop-mediated isothermal amplification coupled with a lateral flow dipstick (LAMP-LFD) assay for rapid detection of Echinostomatidae metacercaria in edible snail samples (2024)
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Development and validation of reverse-transcription cross-priming amplification-based lateral flow assay for the detection of infectious hematopoietic necrosis virus (2024)
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Development of a duplex lateral flow dipstick test for the detection and differentiation of Listeria spp. and Listeria monocytogenes in meat products based on loop-mediated isothermal amplification (2019)
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Development of a lateral flow recombinase polymerase amplification assay for rapid and visual detection of Cryptococcus neoformans/C. gattii in cerebral spinal fluid (2019)
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Development of a Loop-Mediated Isothermal Amplification Assay Coupled With a Lateral Flow Dipstick Test for Detection of Myosin Binding Protein C3 A31P Mutation in Maine Coon Cats (2022)
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Development of a loop-mediated isothermal amplification detection assay for Dictyocaulus viviparus (2024)
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Development of a Rapid and Sensitive CasRx-Based Diagnostic Assay for SARS-CoV-2 (2021)
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Development of a Rapid and Sensitive CasRx-Based Diagnostic Assay for SARS-CoV-2 (2022)
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Development of a rapid and visual detection method for Rickettsia rickettsii combining recombinase polymerase assay with lateral flow test (2018)
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Development of a rapid and visual nucleotide detection method for a Chinese epidemic strain of Orientia tsutsugamushi based on recombinase polymerase amplification assay and lateral flow test (2018)
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Development of a Rapid Sex Identification Method for Newborn Pigeons Using Recombinase Polymerase Amplification and a Lateral-Flow Dipstick on Farm (2022)
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Development of a Recombinase Polymerase Amplification Assay for Schistosomiasis Japonica Diagnosis in the Experimental Mice and Domestic Goats (2021)
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Novel high-performance detection of Raillietina echinobothrida, Raillietina tetragona, and Raillietina cesticillus using loop-mediated isothermal amplification coupled with a lateral flow dipstick (LAMP-LFD) (2021)
Panich, W., Tejangkura, T., & Chontananarth, T.
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Nucleic acid detection of plant genes using CRISPR-Cas13 (2019)
Abudayyeh OO, Gootenberg JS, Kellner MJ, Zhang F
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Nucleic Acid Detection Using CRISPR/Cas Biosensing Technologies. (2020)
Aman R., Mahas A., Mahfouz M.,
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Nucleic Acid Lateral Flow Immunoassays: Nachweisentwicklung, Limitationen und methodische Neuerungen (2021)
Breitbach, A.
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Obtaining Specific Sequence Tags for Yersinia pestis and Visually Detecting Them Using the CRISPR-Cas12a System (2021)
Chen, G., Lyu, Y., Wang, D., Zhu, L., Cao, S., Pan, C., Feng, E., Zhang, W., Liu, X., Cui, Y., & Wang, H.
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One-Step Reverse-Transcription Recombinase Polymerase Amplification Using Lateral Flow Strips for the Detection of Coxsackievirus A6 (2021)
Xie, J., Yang, X., Duan, L., Chen, K., Liu, P., Zhan, W., Zhang, C., Zhao, H., Wei, M., Tang, Y., & Luo, M.
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Optimization of a rapid and sensitive nucleic acid lateral flow biosensor for hepatitis B virus detection (2023)
Abbas Ali Husseini, Serap Yesilkir Baydar
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Optimization of the Isothermal Amplification Method for Mycobacterium Tuberculosis Detection and Visualization for Fieldwork. (2020)
Ağel H.E., Sağcan H., Ceyhan I., Durmaz R.
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PAM-flexible Engineered FnCas9 variants for robust and ultra-precise genome editing and diagnostics (2024)
Acharya, S., Ansari, A.H., Kumar Das, P. et al.
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Paper Device Combining CRISPR/Cas12a and Reverse-Transcription Loop-Mediated Isothermal Amplification for SARS-CoV-2 Detection in Wastewater (2022)
Cao, H., Mao, K., Ran, F., Xu, P., Zhao, Y., Zhang, X., Zhou, H., Yang, Z., Zhang, H., & Jiang, G.
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PNA-Pdx: Versatile Peptide Nucleic Acid-Based Detection of Nucleic Acids and SNPs (2023)
Jiang, W., Aman, R., Ali, Z., Rao, G. S., & Mahfouz, M.
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Point of care colourimetric and lateral flow LAMP assay for the detection of Haemonchus contortus in ruminant faecal samples (2021)
Khangembam, R., Tóth, M., Vass, N., Várady, M., Czeglédi, L., Farkas, R., & Antonopoulos, A.
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Point-of-care detection of Neisseria gonorrhoeae based on RPA-CRISPR/Cas12a (2023)
Tu, Q., Cao, X., Ling, C., Xiang, L., Yang, P., & Huang, S.
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Point-of-care testing for COVID-19 using SHERLOCK diagnostics. (2020)
Joung J., Ladha A., Saito M., et. al.
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Polyethersulfone-Based Microfluidic Device Integrated with DNA Extraction on Paper and Recombinase Polymerase Amplification for the Detection of Salmonella enterica (2023)
Chen, Y., Hu, Y., & Lu, X.
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Preliminary clinical performance of a Cas13a-based lateral flow assay for detecting Neisseria gonorrhoeae in urine specimens (2024)
Allan-Blitz L, Adams G, Sanders G, Shah P, Ramesh K, Jarolimova J, Ard KL, Branda JA, Klausner JD, Sabeti PC, Lemieux JE. 0
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Pseudo-Complementary G:C Base Pair for Mixed Sequence dsDNA Invasion and Its Applications in Diagnostics (2023)
López-Tena, M., Farrera-Soler, L., Barluenga, S., & Winssinger, N.
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Quick hassle-free detection of cyprinid herpesvirus 2 (CyHV-2) in goldfish using recombinase polymerase amplification-lateral flow dipstick (RPA-LFD) assay (2022)
Preena, P. G., Kumar, T. V. A., Johny, T. K., Dharmaratnam, A., & Swaminathan, T. R.
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Rapid analysis of Escherichia coli O157:H7 using isothermal recombinase polymerase amplification combined with triple-labeled nucleotide probes. (2020)
Hu J., Wang Y., Su H., et. al.
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Rapid and accurate detection of novel coronavirusSARS-CoV-2 using CRISPR-Cas3. (2020)
Yoshimi K., Takeshita K., Yamayoshi S., et. al.
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Rapid and accurate nucleobase detection using FnCas9 and its application in COVID-19 diagnosis (2021)
Azhar, M., Phutela, R., Kumar, M., Ansari, A. H., Rauthan, R., Gulati, S., Sharma, N., Sinha, D., Sharma, S., Singh, S., Acharya, S., Sarkar, S., Paul, D., Kathpalia, P., Aich, M., Sehgal, P., Ranjan, G., Bhoyar, R. C.
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Rapid and accurate nucleobase detection using FnCas9 and its application in COVID-19 diagnosis (2021)
Azhar, M., Phutela, R., Kumar, M., Ansari, A. H., Rauthan, R., Gulati, S., Sharma, N., Sinha, D., Sharma, S., Singh, S., Acharya, S., Sarkar, S., Paul, D., Kathpalia, P., Aich, M., Sehgal, P., Ranjan, G., Bhoyar, R. C., Singhal, K., … Maiti, S.
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Rapid and accurate species identification for ecological studies and monitoring using CRISPR-based SHERLOCK. (2020)
Baerwald M., Goodbla A., nagarajan R., Gootenberg J., Abudayyeh O., Zhang F., Schreier A.
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Rapid and highly sensitive LAMP-CRISPR/Cas12a-based identification of bovine mastitis milk samples contaminated by Escherichia coli (2023)
Shaizadinova, A., Amanzholova, M., Kirillov, S., Bulashev, A., & Abeldenov, S.
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Rapid and sensitive detection of Babesia bovis and Babesia bigemina by loop-mediated isothermal amplification combined with a lateral flow dipstick Vet. Parasitol 2016; 219, 71-76 (2016)
Yang Y et al.
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Rapid and sensitive detection of Candidatus Liberibacter asiaticus by loop mediated isothermal amplification combined with a lateral flow dipstick BMC Microbiol. 2014; 1186 (2014)
Rigano AL et al.
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Rapid and sensitive detection of infectious spleen and kidney necrosis virus by loop-mediated isothermal amplification combined with a lateral flow dipstick (2010)
W.C. Ding, Jiong Chen, Y.H. Shi, X.J. Lu, M.Y. Li
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Rapid and sensitive detection of infectious spleen and kidney necrosis virus by recombinase polymerase amplification combined with lateral flow dipsticks. (2020)
Li H., Yuan G., Luo Y., Yu Y., Ai T., Su J.
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Rapid and sensitive detection of Karenia mikimotoi by loop-mediated isothermal amplification combined with a lateral flow dipstick (2021)
Han, X., Zhao, T., Yan, T., & Yu, R.
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Rapid and sensitive detection of Little cherry virus 2 using isothermal reverse transcription-recombinase polymerase amplification (2014)
Mekuria, T. A., Zhang, S., & Eastwell, K. C.
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Rapid and sensitive detection of pathogenic Elizabethkingia miricola in black spotted frog by RPA-LFD and fluorescent probe-based RPA (2022)
Qiao, M., Zhang, L., Chang, J., Li, H., Li, J., Wang, W., Yuan, G., & Su, J.
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Rapid and sensitive diagnosis of live Mycobacterium tuberculosis using clustered regularly interspaced short palindromic repeat-Cas13a point-of-care RNA testing (2024)
Yu Wang, Huihuang Lin, Anqi Yang, Jiaming Huang, Weicong Ren, Jiajun Dong, Shaojie Wang, Wenxue Xu, Yu Pang, Jieming Qu, Jia Liu
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Rapid and sensitive point-of-care diagnosis of human cytomegalovirus infection using RPA-CRISPR technology (2024)
Shin, Kihye et al.
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Rapid and simple detection of Glaesserella parasuis in synovial fluid by recombinase polymerase amplification and lateral flow strip (2019)
Zhang TT, Liu MZ, Yin RH, Yao LQ, Liu BS, Chen ZL
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Rapid and simple detection of Phytophthora cactorum in strawberry using a coupled recombinase polymerase amplification-lateral flow strip assay (2021)
Lu, X., Xu, H., Song, W., Yang, Z., Yu, J., Tian, Y., Jiang, M., Shen, D., & Dou, D.
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Rapid and Visual Detection of Barley Yellow Dwarf Virus by Reverse Transcription Recombinase Polymerase Amplification with Lateral Flow Strips (2022)
Kim, N. K., Lee, H. J., Kim, S. M., & Jeong, R. D.
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Rapid and visual detection of enterovirus using recombinase polymerase amplification combined with lateral flow strips. (2020)
Yang X., Xie J., Hu S., Zhan W., Duan L., Chen K., Zhang C., Yin A., Luo M
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Rapid and Visual Detection of Heterodera schachtii Using Recombinase Polymerase Amplification Combined with Cas12a-Mediated Technology (2021)
Yao, K., Peng, D., Jiang, C., Zhao, W., Li, G., Huang, W., Kong, L., Gao, H., Zheng, J., & Peng, H.
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Rapid and visual detection of Mycoplasma genitalium using recombinase polymerase amplification combined with lateral flow strips (2024)
Pufang Ren, Yingmin Zeng, Yao Feng, Honghai Hong, Yong Xia
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Rapid and visual detection of Mycoplasma synoviae by recombinase-aided amplification assay combined with a lateral flow dipstick (2022)
Xia, W., Chen, K., Liu, W., Yin, Y., Yao, Q., Ban, Y., Pu, Y., Zhan, X., Bian, H., Yu, S., Han, K., Yang, L., Wang, H., & Fan, Z.
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Rapid and visual detection of porcine deltacoronavirus by recombinase polymerase amplification combined with a lateral flow dipstick (2020)
Gao X., Liu X., Zhang Y., et. al.
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Rapid and visual detection of tomato spotted wilt virus using recombinase polymerase amplification combined with lateral flow strips (2021)
Lee, H. J., Cho, I. S., Ju, H. J., & Jeong, R. D.
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Rapid and visual detection of Trichinella spp. using a lateral flow strip-based recombinase polymerase amplification (LF-RPA) assay (2019)
Li TT, Wang JL, Zhang NZ, Li WH, Yan HB, Li L,Jia WZ, Fu BQ
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Rapid and visual detection of Verticillium dahliae using recombinase polymerase amplification combined with lateral flow dipstick. (2020)
Ju Y., Li C., Shen P., et. al.,
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Rapid authentication of Cordyceps by lateral flow dipstick. J. Pharm. Biomed. Anal 2015, 111, 306-310 (2015)
Wong YL et al.
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Rapid detection and differentiation of carp oedema virus and cyprinid herpes virus-3 in koi and common carp. J Fish Dis. 2018 May;41(5):761-772. (2018)
Soliman H. and El-Matoubli M.
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Rapid Detection of 2019 Novel Coronavirus SARS-CoV-2 Using a CRISPR-based DETECTR Lateral Flow Assay. (2020)
Broughton J. P., Deng X., Fasching C.L., Singh J., Streithorst J., Granados, A., Sotomayor-Gonzalez A., Zorn K., Gopez A., Hsu E., Gu W., Miller S., Pan C., Guevara H., Wadford D., Chen J., Chio C.Y.
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Rapid detection of African swine fever virus using Cas12a-based portable paper diagnostics. (2020)
Lu L., Li F., Chen Q., et. al
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Rapid detection of blood using a novel application of RT-RPA integrated with CRISPR-Cas: ALAS2 detection as a model (2024)
Su, Chih-Wen et al.
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Rapid detection of bovine viral diarrhea virus using recombinase polymerase amplification combined with lateral flow dipstick assays in bulk milk (2018)
Hou P, Zhao G, Wang H, He C, He H
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Rapid detection of Burkholderia pseudomallei with a lateral flow recombinase polymerase amplification assay (2019)
Peng Y, Zheng X, Kann B, Li W, Zhang W, Jiang T, Lu J, Qin A
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Rapid detection of cardamom mosaic virus in crude plant extracts using reverse transcription-recombinase polymerase amplification-lateral flow assay (RT-RPA-LFA) (2024)
M. Greeshma, A. I. Bhat
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Rapid detection of Edwardsiella ictaluri in yellow catfish (Pelteobagrus fulvidraco) by real-time RPA and RPA-LFD (2022)
Li, H., Zhang, L., Yu, Y., Ai, T., Zhang, Y., & Su, J.
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Rapid detection of Enterococcus and vancomycin resistance using recombinase polymerase amplification (2021)
Panpru, P., Srisrattakarn, A., Panthasri, N., Tippayawat, P., Chanawong, A., Tavichakorntrakool, R., Daduang, J., Wonglakorn, L., & Lulitanond, A.
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Rapid detection of HIV-1 proviral DNA for early infant diagnosis using recombinase polymerase amplification (2013)
Boyle, D. S., Lehman, D. A., Lillis, L., Peterson, D., Singhal, M., Armes, N., Parker, M., Piepenburg, O., & Overbaugh, J.
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Rapid detection of kdr mutation F1534C in Aedes aegypti using recombinase polymerase amplification and lateral flow dipsticks (2022)
Ahmed, M., Nath, N. S., Hugo, L. E., Devine, G. J., Macdonald, J., & Pollak, N. M.
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Rapid detection of mcr-1 by recombinase polymerase amplification (2018)
Xu, J., Wang, X., Yang, L., Kan, B., & Lu, X.
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Rapid detection of mcyG gene of microcystins producing cyanobacteria in water samples by recombinase polymerase amplification combined with lateral flow strips (2021)
Li, J., Feng, M., & Yu, X.
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Rapid Detection of Measles Virus Using Reverse Transcriptase/Recombinase Polymerase Amplification Coupled with CRISPR/Cas12a and a Lateral Flow Detection: A Proof-of-Concept Study (2024)
Pinchon, E.; Henry, S.; Leon, F.; Fournier-Wirth, C.; Foulongne, V.; Cantaloube, J.-F.
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Rapid detection of methicillin-resistant Staphylococcus aureus in positive blood-cultures by recombinase polymerase amplification combined with lateral flow strip (2022)
Srisrattakarn, A., Panpru, P., Tippayawat, P., Chanawong, A., Tavichakorntrakool, R., Daduang, J., Wonglakorn, L., & Id, A. L.
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Rapid detection of monkeypox virus using a CRISPR-Cas12a mediated assay: a laboratory validation and evaluation study (2023)
Caly, L., Pasricha, S., Williamson, D. A., Kerry MBiotech, W. J., Whitehead, L. W., Williams, L., Cooney, J. P., Pellegrini, M., Savic, I., Prestedge, J., Tran, T., Lim, C. K., Towns, J. M., Bradshaw, C. S., Fairley, C., F Chow, E. P., Chen, M. Y., Jen Low, S., O, M. T., … Williamson, D. A.
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Rapid detection of SARS-CoV-2 RNA using Reverse Transcription Recombinase Polymerase Amplification (RT-RPA) with Lateral Flow for N-protein gene and variant-specific deletion-insertion mutation in S-protein gene (2024)
Malaga et al.
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Rapid Detection of SARS-CoV-2 RNA Using Reverse Transcription Recombinase Polymerase Amplification (RT-RPA) with Lateral Flow for N-Protein Gene and Variant-Specific Deletion–Insertion Mutation in S-Protein Gene (2023)
Tanuri, A., Malaga, J. L., Pajuelo, M. J., Okamoto, M., Kagning Tsinda, E., Otani, K., Tsukayama, P., Mascaro, L., Cuicapuza, D., Katsumi, M., Kawamura, K., Nishimura, H., Sakagami, A., Ueki, Y., Omiya, S., Okamoto, S., Nakayama, A., Fujimaki, S., Yu, C., … Saito, M.
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Rapid detection of Singapore grouper iridovirus by a recombinase polymerase amplification combined with lateral flow dipstick (2024)
PAN Ying, YANG Jiahui, PENG Fayong, HUANG Youhua, QIN Qiwei, HUANG Xiaohong
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Rapid detection of Singapore grouper iridovirus by a recombinase polymerase amplification combined with lateral flow dipstick (2024)
PAN Ying, YANG Jiahui, PENG Fayong, HUANG Youhua, QIN Qiwei, HUANG Xiaohong
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Rapid Detection of Strawberry Mild Yellow Edge Virus with a Lateral Flow Strip Reverse Transcription Recombinase Polymerase Amplification Assay (2022)
Zou, X., Dong, C., Ni, Y., & Gao, Q.
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Rapid detection of strawberry mottle virus using reverse transcription recombinase polymerase amplification with lateral flow strip (2022)
Zou, X., Dong, C., Ni, Y., Yuan, S., & Gao, Q. H.
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Rapid detection of the rice false smut fungus Ustilaginoidea virens by lateral flow strip-based recombinase polymerase amplification assay (2023)
Jia-cheng, X., San-lian, W., Yue, L., Yuan-di, X., Jing, Y., Ting, Z., Jia-jia, C., Zheng-guang, Z., Dan-yu, S., & Hai-feng, Z.
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Rapid Detection of the Strawberry Foliar Nematode Aphelenchoides fragariae Using Recombinase Polymerase Amplification Assay with Lateral Flow Dipsticks (2024)
Sergei A. Subbotin
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Rapid Detection of the Varicella-Zoster Virus Using a Recombinase-Aided Amplification-Lateral Flow System (2022)
Bienes, K. M., Mao, L., Selekon, B., Gonofio, E., Nakoune, E., Wong, G., & Berthet, N.
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Rapid Diagnosis and Visual Detection of Potato Cyst Nematode (Globodera rostochiensis) Using Recombinase Polymerase Amplification Combination with Lateral Flow Assay Method (RPA-LFA) (2022)
Wang, X. ;, Lei, R. ;, Peng, H. ;, Jiang, R. ;, Shao, H. D. ;, Peng, J. J. ;, Wang, X., Lei, R., Peng, H., Jiang, R., Shao, H., Ge, J., & Peng, D.
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Rapid diagnosis of two marine viruses, red sea bream iridovirus and viral hemorrhagic septicemia virus by PCR combined with lateral flow assay. (2020)
Seo H., Kil E., Fadhila C., et. al.
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Rapid Diagnostic Tests for the Detection of the Four Dengue Virus Serotypes in Clinically Relevant Matrices (2023)
Pollak, N. M., Olsson, M., Ahmed, M., Tan, J., Lim, G., Setoh, Y. X., Wong, J. C. C., Lai, Y. L., Hobson-Peters, J., Macdonald, J., & McMillan, D.
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Rapid DNA extraction and colorimetric amplicon visualisation speed up LAMP-based detection of soybean allergen in foods (2023)
Schäfer, L., Allgöwer, S., & Holzhauser, T.
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Rapid isothermal point-of-care test for screening of SARS-CoV-2 (2023)
Zingg, J.-M., Yang, Y.-P., Seely, S., Joshi, P., Roshid, M. H. O., Iribarren Latasa, F., O’Connor, G., Alfaro, J., Riquelme, E., Bernales, S., Dikici, E., Deo, S., & Daunert, S.
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Rapid low-resource detection of Plasmodium falciparum in infected Anopheles mosquitoes (2024)
Hugo Leon E. , van Huyssteen Karla , Oloniniyi Olamide , Donnelly Laura , Conn Anna , Collins Katharine A. , Mitchell Hayley , McCarthy James S. , Macdonald Joanne
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Rapid molecular assays for the detection of the four dengue viruses in infected mosquitoes (2022)
Ahmed, M., Pollak, N. M., Hugo, L. E., van den Hurk, A. F., Hobson-Peters, J., Macdonald, J., & Paradkar, P. N.
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Rapid molecular detection of CMY-2, and CTX-M group 1 and 9 variants via recombinase polymerase amplification (2023)
Ertl, N. G., Irwin, A. D., Macdonald, J., Bauer, M. J., Wang, C. Y. T., Harris, P. N. A., Heney, C., Zowawi, H. M., & Whiley, D. M.
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Rapid onsite detection of piper yellow mottle virus infecting black pepper by recombinase polymerase amplification-lateral flow assay (2023)
Greeshma, M., Bhat, A. I., & Jeevalatha, A.
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Rapid PCR-lateral flow assay for the onsite detection of Atlantic white shrimp (2023)
Kwawukume, S., Velez, F. J., Williams, D., Cui, L., & Singh, P.
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Rapid RNase H-dependent PCR lateral flow assay for the detection of red snapper (2023)
Samuel Kwawukume, Frank J. Velez, Nethraja Kandula, David Williams, Leqi Cui, Prashant Singh
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Rapid sample preparation and low-resource molecular detection of hepatopancreatic parvoviruses (HPV) by recombinase polymerase amplification lateral flow detection assay in shrimps (Fenneropenaeus merguiensis). (2022)
Pollak ID, N. M., Fais, O., Kristoffersen, J., Phuthaworn, C., Knibb, W., & Macdonald, J. I.
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Rapid SARS-CoV-2 testing in primary material based on a novel multiplex LAMP assay (2020)
Schermer B., Fabretti F., Damagnez M., et. al.
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Rapid Visual CRISPR Assay: A Naked-Eye Colorimetric Detection Method for Nucleic Acids Based on CRISPR/Cas12a and a Convolutional Neural Network (2022)
Xie, S., Tao, D., Fu, Y., Xu, B., Tang, Y., Steinaa, L., Hemmink, J. D., Pan, W., Huang, X., Nie, X., Zhao, C., Ruan, J., Zhang, Y., Han, J., Fu, L., Ma, Y., Li, X., Liu, X., & Zhao, S.
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Rapid visual detection of cyprinid herpesvirus 2 by recombinase polymerase amplification combined with a lateral flow dipstick (2018)
Wang H, Sun M, Xu D, Podok P, Xie J, Jiang Y, Lu L
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Rapid visual detection of Enterocytozoon hepatopenaei by recombinase polymerase amplification combined with a lateral flow dipstick (2022)
Pang, J., Zhao, C., Liu, Z., Lu, Q., He, X., Weng, S., & Jianguo
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Rapid visual detection of Mycobacterium avium subsp. paratuberculosis by recombinase polymerase amplification combined with a lateral flow dipstick (2018)
Zhao G, Wang H, Hou P, He C, He H
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Rapid Visual Detection of Pathogenic Streptococcus suis Type 2 through a Recombinase Polymerase Amplification Assay Coupled with Lateral Flow Test (2022)
Qi, Y., Li, W., Li, X., Shen, W., Lv, R., Lu, N., Li, J., Zhuang, S., Xu, Y., Gui, Q., Shen, H., & Li, Y.
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Rapid Visual Detection of Peronophythora litchii on Lychees Using Recombinase Polymerase Amplification Combined with Lateral Flow Assay Based on the Unique Target Gene Pl_101565 (2024)
Wang, R.; Li, B.; Shi, M.; Zhao, Y.; Lin, J.; Chen, Q.; Liu, P.
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Rapid Visual Detection of Red Sea Bream Iridovirus Using a Novel Cross-Priming Amplification-Based Lateral Flow Assay (2024)
Guk Hyun Kim, Dong Jun Shin, Ji Yeong Choi, Hyun Deok Choi, Joon Gyu Min, Kwang Il Kim
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Rapid Visual Detection of Streptococcus suis and Actinobacillus pleuropneumoniae Through Duplex Recombinase Polymerase Amplification Combined with Lateral Flow Dipsticks (2022)
Zhang, S., Xie, H., Liu, M., Zheng, A., Yan, H., Duan, M., Wei, X., Teng, Z., Zhang, H., & Xia, X.
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Rapid visual nucleic acid detection of Vibrio alginolyticus by recombinase polymerase amplification combined with CRISPR/Cas13a. (2023)
Wang, Y., Hou, Y., Liu, X., Lin, N., Dong, Y., Liu, F., Xia, W., Zhao, Y., Xing, W., Chen, J., & Chen, C.
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RAPID-CRISPR: Highly Sensitive Diagnostic Assay for Detection of PML-RARA Isoforms in Acute Promyelocytic Leukemia (2024)
Akash Maity, Amritha Sathyanarayanan, Rohit Kumar, Jesal Vora, Jitendra Gawde, Hasmukh Jain, Dr., Bhausaheb Bagal, Papagudi G Subramanian, Manju Sengar, MD, DM, Navin Khattry, Nikhil Patkar, Syed Khizer Hasan
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Rapid, adaptable and sensitive Cas13-based COVID-19 diagnostics using ADESSO (2022)
Casati, B., Verdi, J. P., Hempelmann, A., Kittel, M., Klaebisch, A. G., Meister, B., Welker, S., Asthana, S., Giorgio, S. di, Boskovic, P., Man, K. H., Schopp, M., Ginno, P. A., Radlwimmer, B., Stebbins, C. E., Miethke, T., Papavasiliou, F. N., & Pecori, R.
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Rapid, CRISPR-based, field-deployable detection of white spot syndrome virus in shrimp (2019)
Sullivan TJ, Arun KD, Cruz-Flores R, Bodnar AG
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Rapid, easy, sensitive, low-cost and on-site detection of environmental DNA and RNA using CRISPR-Cas13 (2024)
Jiwei Yang, Shoma Matsushita, Fei Xia, Susumu Yoshizawa, Wataru Iwasaki
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Rapid, Sensitive, and Specific Severe Acute Respiratory Syndrome Coronavirus 2 Detection: A Multicenter Comparison Between Standard Quantitative Reverse-Transcriptase Polymerase Chain Reaction and CRISPR-Based DETECTR (2021)
Brandsma, E., Verhagen, H., van de Laar, T., Claas, E., Cornelissen, M., & van den Akker, E.
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Rapid, sensitive, and specific, low-resource molecular detection of Hendra virus (2023)
Pollak, N. M., Marsh, G. A., Olsson, M., McMillan, D., & Macdonald, J.
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Rational Programming of Cas12a for Early-Stage Detection of COVID-19 by Lateral Flow Assay and Portable Real-Time Fluorescence Readout Facilities (2022)
Yi, Z., de Dieu Habimana, J., Mukama, O., Li, Z., Odiwuor, N., Jing, H., Nie, C., Hu, M., Lin, Z., Wei, H., & Zeng, L.
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Recombinase Polymerase Amplification and Lateral Flow Assay for Ultrasensitive Detection of Low-Density. (2020)
Lalremruata A., Nguyen T., MCCall M., et. al.
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Recombinase polymerase amplification assay combined with a dipstick-readout for rapid detection of Mycoplasma ovipneumoniae infections (2021)
Gupta, S. K., Deng, Q., Gupta, T. B., Maclean, P., Jores, J., Heiser, A.
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Recombinase polymerase amplification assay combined with a lateral flow dipstick for rapid detection of Tetracapsuloides bryosalmonae, the causative agent of proliferative kidney disease in salmonids. Parasit Vectors. 2018 Apr 11;11(1):234. (2018)
Soliman H. et al.
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Recombinase polymerase amplification assays with lateral flow dipsticks for rapid detection of the reniform nematode, Rotylenchulus reniformis, and the burrowing nematode, Radopholus similis (2024)
Sergei A Subbotin, Renato N Inserra
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Recombinase polymerase amplification combined with a lateral flow dipstick for discriminating between infectious Penaeus stylirostris densovirus and virus-related sequences in shrimp genome (2014)
Jaroenram, W., & Owens, L.
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Recombinase Polymerase Amplification Combined with Lateral Flow Dipstick Assay for the Rapid and Sensitive Detection of Pseudo-nitzschia multiseries (2024)
Yuqing Yao, Ningjian Luo, Yujie Zong, Meng Jia, Yichen Rao, Hailong Huang, Haibo Jiang
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Recombinase Polymerase Amplification Combined with Lateral Flow Strip for Rapid Detection of OXA-48-like Carbapenemase Genes in Enterobacterales (2022)
Hemwaranon, P., Srisrattakarn, A., Lulitanond, A., Tippayawat, P., Tavichakorntrakool, R., Wonglakorn, L., Daduang, J., & Chanawong, A.
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Recombinase polymerase amplification lateral flow dipstick (RPA-LF) detection of Babesia orientalis in water buffalo (Bubalus babalis, Linnaeus, 1758) (2021)
An, X., Zhao, Y., Cui, J., Liu, Q., Yu, L., Zhan, X., Zhang, W., He, L., & Zhao, J.
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Recombinase polymerase amplification with lateral flow strip for detecting Babesia microti infections (2021)
Nie, Z., Zhao, Y., Shu, X., Li, D., Ao, Y., Li, M., Wang, S., Cui, J., An, X., Zhan, X., He, L., Liu, Q., & Zhao, J.
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Recombinase polymerase amplification-lateral flow (RPA-LF) assay combined with immunomagnetic separation for rapid visual detection of Vibrio parahaemolyticus in raw oysters. (2020)
Jiang W., Ren Y., Han X., Xue J., Shan T., Chen Z., Liu Y., Quan W.
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Recombinase Polymerase Amplification/Cas12a-Based Identification of Xanthomonas arboricola pv. pruni on Peach (2021)
Luo, M., Meng, F. Z., Tan, Q., Yin, W. X., & Luo, C. X.
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Recombinase-aid amplification combined with lateral flow detection assay for sex identification of the great white pelican (Pelecanus onocrotalus) (2024)
Zeng, F., Chen, X., Zhong, W. et al.
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REVEALR: A Multicomponent XNAzyme-Based Nucleic Acid Detection System for SARS-CoV-2 (2021)
Yang, K., & Chaput, J. C.
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Reverse transcription loop-mediated isothermal amplification combined with lateral flow device (RT-LAMP-LFD) for swine influenza virus detection (2021)
Asih, A. U., Janetanakit, T., Nasamran, C., Bunpapong, N., Boonyapisitsopa, S., Amonsin, A.
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Reverse Transcription-Loop-Mediated Isothermal Amplification-CRISPR-Cas13a Technology as a Promising Diagnostic Tool for SARS-CoV-2 (2022)
Ortiz-Cartagena, C., Fernández-García, L., Blasco, L., Pacios, O., Bleriot, I., López, M., Cantón, R., & Tomás, M.
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RT-RPA Assay Combined with a Lateral Flow Strip to Detect Soybean Mosaic Virus (2024)
Oh BG, Yoon JY, Ju HJ
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RT-RPA-Cas12a-based assay facilitates the discrimination of SARS-CoV-2 variants of concern (2023)
Tang, G., Zhang, Z., Tan, W., Long, F., Sun, J., Li, Y., Zou, S., Yang, Y., Cai, K., Li, S., Wang, Z., Liu, J., Mao, G., Ma, Y., Zhao, G.-P., Tian, Z.-G., & Zhao, W.
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SARS-CoV‑2 and Its Omicron Variants Detection with RT-RPA -CRISPR/Cas13a-Based Method at Room Temperature (2024)
Li J, Wang X, Chen L, Duan L, Tan F, Li K et al .
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Sensitive and Easy-Read CRISPR Strip for COVID-19 Rapid Point-of-Care Testing (2021)
Li, H., Dong, X., Wang, Y., Yang, L., Cai, K., Zhang, X., Kou, Z., He, L., Sun, S., Li, T., Nie, Y., Li, X., & Sun, Y.
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Sensitive and rapid detection of Babesia species in dogs by recombinase polymerase amplification with lateral flow dipstick (RPA-LFD) (2022)
Onchan, W., Ritbamrung, O., Changtor, P., Pradit, W., Chomdej, S., Nganvongpanit, K., Siengdee, P., Suyasunanont, U., & Buddhachat, K
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Sensitive and Rapid Detection of Citrus Scab Using an RPA-CRISPR/Cas12a System Combined with a Lateral Flow Assay (2021)
Shin, K., Kwon, S. H., Lee, S. C., & Moon, Y. E.
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Sensitive and Specific Exonuclease III-Assisted Recombinase-Aided Amplification Colorimetric Assay for Rapid Detection of Nucleic Acids (2023)
Zhou, Y., Zhang, J., Sun, H., Tao, D., Xu, B., Han, X., Ren, R., Ruan, J., Steinaa, L., Hemmink, J. D., Han, J., Li, X., Xu, J., Zhao, S., Xie, S., & Zhao, C.
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Sensitive naked-eye detection of telomerase activity based on exponential amplification reaction and lateral flow assay (2022)
Cheng, X. R., Wang, F., Liu, C. yun, Li, J., Shan, C., Wang, K., Wang, Y., Li, P. F., & Li, X. M
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Sequence-Specific Detection of ORF1a, BRAF, and ompW DNA Sequences with Loop Mediated Isothermal Amplification on Lateral Flow Immunoassay Strips Enabled by Molecular Beacons (2021)
Varona, M., Eitzmann, D. R., & Anderson, J. L.
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SHERLOCK: nucleic acid detection with CRISPR nucleases (2019)
Kellner MJ, Koob JG, Gootenberg JS, Abudayyeh OO, Zhang F
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SHERLOCK4HAT: A CRISPR-based tool kit for diagnosis of Human African Trypanosomiasis (2022)
Sima, N., Dujeancourt-Henry, A., Perlaza, B. L., Ungeheuer, M.-N., Rotureau, B., & Glover, L.
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Simplified Cas13-based assays for the fast identification of SARS-CoV-2 and its variants (2022)
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Specific and Sensitive Visual Proviral DNA Detection of Major Pathogenic Avian Leukosis Virus Subgroups Using CRISPR-Associated Nuclease Cas13a (2024)
Xu, Qingqing, Yaoyao Zhang, Yashar Sadigh, Na Tang, Jiaqian Chai, Ziqiang Cheng, Yulong Gao, Aijian Qin, Zhiqiang Shen, Yongxiu Yao, and et al.
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Specific Detection of Influenza A and B Viruses by CRISPR-Cas12a-Based Assay (2021)
Park, B. J., Park, M. S., Lee, J. M., & Song, Y. J.
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Streamside detection of Chinook salmon (Oncorhynchus tshawytscha) environmental DNA with CRISPR technology (2024)
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Supporting Information Clustered Regularly Interspaced Short Palindromic Repeats / Cas9- Mediated Lateral Flow Nucleic Acid Assay. (2020)
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The development of highly specific and sensitive primers for the detection of potentially allergenic soybean (Glycine max) using loop-mediated isothermal amplification combined with lateral flow dipstick (LAMP-LFD). (2020)
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The Development of Loop-Mediated Isothermal Amplification Combined with Lateral Flow Dipstick for Detection of Karlodinium veneficum. Harmful Algae 2017; 62, 20-29. (2017)
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Time course of detection of human male DNA from stained blood sample on various surfaces by Loop Mediated Isothermal Amplification and Polymerase Chain Reaction (2018)
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Topological barrier to Cas12a activation by circular DNA nanostructures facilitates autocatalysis and transforms DNA/RNA sensing (2024)
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Toward a CRISPR-Based Point-of-Care Test for Tomato Brown Rugose Fruit Virus Detection (2022)
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Trends and Innovations in Biosensors for COVID-19 Mass Testing (2020)
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Ultrasensitive ImmunoMag-CRISPR Lateral Flow Assay for Point-of-Care Testing of Urinary Biomarkers (2023)
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Use of a recombinase polymerase amplification commercial kit for rapid visual detection of Pasteurella multocida (2019)
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Use of reverse transcription loop-mediated isothermal amplification combined with lateral flow dipstick for an easy and rapid detection of Jembrana disease virus. Virusdisease. 2015 Sep; 26(3):189-95 (2015)
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Utilization of recombinase polymerase amplification combined with a lateral flow strip for detection of Perkinsus beihaiensis in the oyster Crassostrea hongkongensis (2019)
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Variety Differentiation: Development of a CRISPR DETECTR Method for the Detection of Single Nucleotide Polymorphisms (SNPs) in Cacao (Theobroma cacao) and Almonds (Prunus dulcis) (2023)
Wax, N., La-Rostami, F., Albert, C., & Fischer, M.
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Vigilant: An Engineered VirD2-Cas9 Complex for Lateral Flow Assay-Based Detection of SARS-CoV2 (2021)
Marsic, T., Ali, Z., Tehseen, M., Mahas, A., Hamdan, S., & Mahfouz, M.
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Visual Detection of Canine Parvovirus Based on Loop-Mediated Isothermal Amplification Combined with Enzyme-Linked Immunosorbent Assay and with Lateral Flow Dipstick. J. Vet. Med. Sci. 2014 (2014)
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Visual detection of porcine reproductive and respiratory syndrome virus using CRISPR-Cas13a. (2020)
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Videos
FAQs
Q:
Can I use FAM instead of FITC as a label of the probes or primers?
A:
Yes, but initially negative strips may show a faint band after prolonged time, while positive bands will not disappear! We recommend to take an image of the strip shortly after the incubation time is over.
Q:
What is the difference between HybriDetect and HybriDetect 2T?
A:
HybriDetect allows the detection of 1 target on the strip, while HybriDetect 2T allows the detection of 2 targets on the same strip.
Q:
What is the sensitivity of HybriDetect compared to an agarose gel?
A:
This depends on the size of the amplicon. The shorter the amplicon is, the lower is the sensitivity of the agarose gel. If the amplicon is 100 bases long HybriDetect could possibly be 1.000 fold more sensitive compared to the agarose gel.
Q:
Can I still interpret the results of the strips after a prolonged time, e.g. 1 day?
A:
Yes, but initially negative strips may show a faint band after prolonged time, while positive bands will not disappear! We recommend to take an image of the strip shortly after the incubation time is over.
Q:
Do you accept credit card for payment?
A:
Yes, we accept credit card for payment if you purchase our products online via our webshop.
Q:
Do you have a MSDS available for the HybriDetect products?
A:
Yes, you can download the pdf-file of the MSDS for HybriDetect here here and for HybriDetect 2T here.
Q:
How long does it take from the time of my order to shipping?
A:
The products are in inventory and we ship the same day if we receive the order prior 12.00 CET.
Q:
Is it necessary that the kits are shipped at 2-8°C?
A:
No, kits can be shipped at ambient temperatures, but should be stored at 2 – 8°C after receipt.
Q:
I received my kits 3 weeks after you shipped them. Do they still perform well?
A:
Yes, the kits will still perform well. We have made stability tests and could demonstrate that the kits still perform well after storing them for 100 days at 37°C.
Q:
What should be the volume of the sample and buffer I am using in order to run HybriDetect?
A:
The sum of the volume of the sample and the buffer should not exceed 100 µl.
Q:
I am doing an amplification and see that all of my samples are giving positive results. What is the reason for this?
A:
This phenomenon is due to the formation of primer dimers. In this event biotinylated and FITC-labeled primers bind to each other and form dimers resulting in false positive results. You need to redesign your primers in order to prevent primer dimerization.
Q:
What is the shelf life of HybriDetect kits from the time of production?
A:
The shelf life of the kits from the time of production is 24 months. Milenia Biotec is manufacturing fresh lots twice a year, meaning that our customers usually get kits with a remaining shelf life of not less than 12 months.
Q:
Are there any compounds known which may result in an interference with the test or its performance?
A:
Yes, in case the concentration of labeled primers or probes is too high, this may lead to a decreased sensitivity of the test. In this case primer or probe concentration need to be reduced. Furthermore, components of the sample or the sample buffer, including SDS, DTT or urea may have a negative impact on the performance of the test. In this case the sample can be diluted in order to „dilute the effect out“. However this approach will result in a reduced sensitivity of the test. It is also possible to develop a modified assay buffer to reduce the interferences.
Q:
Can HybriDetect be used at elevated temperatures, like hybridizing conditions?
A:
Yes, we demonstrated that HybriDetect (MGHD 1) still performs well at temperatures up to 75°C and HybriDetect 2T at temperatures up to 65°C.
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