Schistosoma mansoni - IgG ELISA

Name: Schistosoma mansoni
Brand: Milenia Biotec GmbH
SKU: This ELISA kit is
Price: 559 EUR
Availability: InStock

559.00 €

(Price excludes VAT and shipping costs)

12 x 8 strips (96 tests)

This ELISA kit is intended for the quantitative detection of IgG antibodies against Schistosoma mansoni and Schistosoma haematobium in human serum.

This product is only available in Germany!

Overview

Name of Product

Schistosoma mansoni - IgG ELISA

Catalog Number

AF 9600

Short Info

This ELISA kit is intended for the quantitative detection of IgG antibodies against Schistosoma mansoni and Schistosoma haematobium in human serum.

This product is only available in Germany!

Method/Platform

ELISA in microplate format

Range/Assay Sensivity

pNPP, λ=405 nm

Test Principle

Specific antibodies in the sample will bind to Schistosoma mansoni antigens sensitized on microtiter plates. The presence of parasite specific antibodies is detected with a Protein A alkaline phosphatase conjugate.

Documents

Additional information (external link)

Protocol

Brief Instructions

Step 1: Blocking 5-15 min at room temperature

Step 2: Incubation with samples 30 min at 37°C

Step 3: Incubation with conjugate 30 min at 37°C

Step 4: Incubation with substrate 30 min at 37°C

Step 5: Measurement of absorbences

Storage

2-8°C

Components

ELISA wells, dilution buffer, washing solution, control sera, conjugate, substrate solution, enzyme buffer, stopping solution

References

Serodiagnosis of Schistosoma mansoni infections in endemic area of Burkina Faso: performance of several immunological tests with different parasite antigens. Acta Tropica. 93, 169-180. (2005)
Sorgho, H., Bahgat, M., Poda, J., Song, W., Kristen, C., Doenhoff, M.J., Zongo, I., Ouedraogo, J. and Ruppel, A.
Serological speciation of human schistosome infections by ELISA with a panel of three antigens. J. Clin. Pathol. 57, 1193-1196. (2004)
Turner, P., Lalloo, K., Bligh, J., Amstrong, M., Whitty, C.J.M., Doenhoff, M.J. and Chiodini, P.L.
The detection of antibodies against Schistosoma mansoni soluble egg antigens (SEA) and CEF6 in ELISA, before and after chemotherapy. Ann. Trop. Med. Parasitol. 97, 697-709. (2003)
Doenhoff, M.J., Wheeler, J.G., Tricker, K., Hamilton, J.V., Sturrock, R.F., Butterworth, A.E., Ouma, J.H., Mbugua, G.G., Kariuki, C. and Koech, D.

Schistosoma mansoni - IgG ELISA

About us

Service

Payment options