Echinococcus multilocularis (Em18) - IgG ELISA

Name: Echinococcus multilo
Brand: Milenia Biotec GmbH
SKU: This ELISA kit is
Price: 559 EUR
Availability: InStock

559.00 €

(Price excludes VAT and shipping costs)

12 x 8 strips (96 tests)

This ELISA kit is intended for the quantitative detection of IgG antibodies against Em-18 antigen of Echinococcus multilocularis in human serum.

This product is only available in Germany!

Overview

Name of Product

Echinococcus multilocularis (Em18) - IgG ELISA

Catalog Number

AF 9310

Short Info

This ELISA kit is intended for the quantitative detection of IgG antibodies against Em-18 antigen of Echinococcus multilocularis in human serum.

This product is only available in Germany!

Method/Platform

ELISA in microplate format

Range/Assay Sensivity

pNPP, λ=405 nm

Test Principle

Specific antibodies in the sample bind to Echinococcus multilocularis Em18 antigen sensitized on microtiter plates. The presence of parasite specific antibodies is detected with a Protein A – alkaline phosphatase conjugate.

Documents

Additional information (external link)

Protocol

Brief Instructions

Step 1: Blocking 5-15 min at room temperature

Step 2: Incubation with samples 30 min at 37°C

Step 3: Incubation with conjugate 30 min at 37°C

Step 4: Incubation with substrate 30 min at 37°C

Step 5: Measurement of absorbences

Storage

2-8°C

Components

ELISA wells, dilution buffer, washing solution, control sera, conjugate, substrate solution, stopping solution

References

Close Relationship Between Clinical Regression and Specific Serology in the Follow-Up of Patients With Alveolar Echinococcosis in Different Clinical Stages (2009)
Tappe, D., Frosch, M., Sako, Y. Itoh, S., Gruner, B., Reuter, S., Nakao, M., Ito, A., Kern, P.
Immunoglobulin G Subclass Responses to Recombinant Em18 in the Follow-Up of Patients With Alveolar Echinococcosis in Different Clinical Stages (2010)
Tappe, D., Sako, Y., Itoh, S. Frosch, M., Gruner, B., Kern, P., Ito, A.
Immunosurveillance of Alveolar Echinococcosis by Specific Humoral and Cellular Immune Tests: Long-Term Analysis of the Swiss Chemotherapy Trial (1976-2001) (2004)
Ammann, R.W., Rebber, E.C., Gottstein, B., Grimm, F. Eckert, J., Renner, E.L.
Outcome after discontinuing long-term benzimidazole (2015)
Ammann, R.W., Stumpe, K.D.M., Grimm, F. Deplazes, P., Huber, S., Bertogg, K., et al.
Serological Monitoring of Progression of Alveolar Echinococcosis With Multiorgan Involvement by Use of Recombinant Em18 (2009)
Ishikawa, Y., Sako, Y. Itoh, S., Ohtake, T., Kohgo, Y., Matsuno, T., Ohsaki, Y., Miyokawa, N., Nakao, M., Nakaya, K., Ito, A.

Echinococcus multilocularis (Em18) - IgG ELISA

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