Echinococcus multi­loc­u­laris Em18 — IgG

This ELISA kit is intended for the quanti­tative detection of IgG antibodies against Em-​​18 antigen of Echinococcus multi­loc­u­laris in human serum.

This product is only available in Germany!

Name of Product
Echinococcus multi­loc­u­laris Em18 — IgG
Catalog Number
AF 9310
Short Info

This ELISA kit is intended for the quanti­tative detection of IgG antibodies against Em-​​18 antigen of Echinococcus multi­loc­u­laris in human serum.

This product is only available in Germany!

Method/​Patform
ELISA in microplate format
Range/​Assay Sensivity
pNPP, λ=405 nm
Test Principle

Specific antibodies in the sample bind to Echinococcus multi­loc­u­laris Em18 antigen sensi­tized on microtiter plates. The presence of parasite specific antibodies is detected with a Protein A – alkaline phosphatase conjugate.

Brief Instruc­tions

Step 1: Blocking 5 – 15 min at room temper­ature

Step 2: Incubation with samples 30 min at 37°C

Step 3: Incubation with conjugate 30 min at 37°C

Step 4: Incubation with substrate 30 min at 37°C

Step 5: Measurement of absorbences

Storage
2 – 8°C
Compo­nents
ELISA wells, dilution buffer, washing solution, control sera, conjugate, substrate solution, stopping solution