HybriDetect - Universal Lateral Flow Assay Kit

Yes, but initially negative strips may show a faint band after prolonged time, while positive bands will not disappear! We recommend to take an image of the strip shortly after the incubation time is over.

HybriDetect allows the detection of 1 target on the strip, while HybriDetect 2T allows the detection of 2 targets on the same strip.

This depends on the size of the amplicon. The shorter the amplicon is, the lower is the sensitivity of the agarose gel. If the amplicon is 100 bases long HybriDetect could possibly be 1.000 fold more sensitive compared to the agarose gel.

Yes, but initially negative strips may show a faint band after prolonged time, while positive bands will not disappear! We recommend to take an image of the strip shortly after the incubation time is over.

Yes, we accept credit card for payment if you purchase our products online via our webshop.

Yes, you can download the pdf-file of the MSDS for HybriDetect here here and for HybriDetect 2T here.

The products are in inventory and we ship the same day if we receive the order prior 12.00 CET.

No, kits can be shipped at ambient temperatures, but should be stored at 2 – 8°C after receipt.

Yes, the kits will still perform well. We have made stability tests and could demonstrate that the kits still perform well after storing them for 100 days at 37°C.

The sum of the volume of the sample and the buffer should not exceed 100 µl.

This phenomenon is due to the formation of primer dimers. In this event biotinylated and FITC-labeled primers bind to each other and form dimers resulting in false positive results. You need to redesign your primers in order to prevent primer dimerization.

The shelf life of the kits from the time of production is 24 months. Milenia Biotec is manufacturing fresh lots twice a year, meaning that our customers usually get kits with a remaining shelf life of not less than 12 months.

Yes, in case the concentration of labeled primers or probes is too high, this may lead to a decreased sensitivity of the test. In this case primer or probe concentration need to be reduced. Furthermore, components of the sample or the sample buffer, including SDS, DTT or urea may have a negative impact on the performance of the test. In this case the sample can be diluted in order to „dilute the effect out“. However this approach will result in a reduced sensitivity of the test. It is also possible to develop a modified assay buffer to reduce the interferences.

Yes, we demonstrated that HybriDetect (MGHD 1) still performs well at temperatures up to 75°C and HybriDetect 2T at temperatures up to 65°C.