HybriDetect
The HybriDetect is a simple and quick tool to develop your own rapid test. Various molecules can be detected: Proteins, Antibodies, Genetic amplicons
Name of Product
HybriDetect
Catalog Number
MGHD 1
Short Info
The HybriDetect is a simple and quick tool to develop your own rapid test. Various molecules can be detected: Proteins, Antibodies, Genetic amplicons
Method/Patform
Lateral flow, sandwich or competetive
Range/Assay Sensivity
5 pg DNA, eqivalent to agarose gel electrophoresis
Test Principle
HybriDetect is a ready-to-use, universal test strip (dipstick) based on lateral flow technology using gold particles. The dipstick can be used to develop qualitative or quantitative rapid test systems for a wide range of analytes such as antibodies, gene amplification products or proteins . The results can be interpreted qualitative or quantitative.
Brief Instructions
Storage
2 – 8°C
Components
HybriDetect dipsticks, HybriDetect assay buffer
- Abudayyeh, O. O., Gootenberg, J. S., Kellner, M. J., & Zhang, F. (2019). Nucleic Acid Detection of Plant Genes Using CRISPR-Cas13. The CRISPR Journal, 2(3), 165 – 171.
- Ahmed F. A. et al., Genome-informed diagnostics for specific and rapid detection of Pectobacterium species using recombinase polymerase amplification coupled with a lateral flow device. Sci Rep. 2018 Oct 29;8(1):15972
- Bai, J., Lin, H., Li, H., Zhou, Y., Liu, J., Zhong, G., … Huang, L. (2019). Cas12a-Based On-Site and Rapid Nucleic Acid Detection of African Swine Fever. Frontiers in Microbiology, 10(December), 1 – 9.
- Castellanos-Gonzalez A et al., A Novel Test to Diagnose Canine Visceral Leishmaniasis at the Point of Care. Am. J. Med Hyg. 2015, 93 (5), 970 – 975
- Chao CC et al., Development of Recombinase Polymerase Amplification Assays for Detection of Orienta Tsutsugamushi or Rickettsia typhi. PLoS Negl. Trop. Dis. 2015; 9 (7)
- Cordray MS et al., A Paper and Plasitc Device for the Combined Isothermal Amplification and Lateral Flow Detection of Plasmodium DNA. Malar. J. 2015; 14, 472
- Ding W. C., Chen J., Shi Y. H., Lu X. J., Li M. Y.
- Huang H. L. et al., Detection of Skeletonema costatum based on loop-mediated isothermal amplification combined with lateral flow dipstick. Mol Cell Probes. 2017 Dec;36:36 – 42.
- Huang HL et al., The Development of Loop-Mediated Isothermal Amplification Combined with Lateral Flow Dipstick for Detection of Karlodinium veneficum. Harmful Algae 2017; 62, 20 – 29.
- Kaewphinit T., Arunrut N., Kiatpathomchai W., Santiwatanakul S., Jaratsing P., Chansiri K., Detection of Mycobacterium tuberculosis by using loop-mediated isothermal amplification combined with a lateral flow dipstick in clinical samples. Biomed. Res. Int. 2013; 1 – 6
- Kanchanaphum P., Time Course of Detection of Human Male DNA from Stained Blood Sample on Various Surfaces by Loop Mediated Isothermal Amplification and Polymerase Chain Reaction. Biomed Res Int. 2018 Mar 22;2018:2981862
- Kellner, M. J., Koob, J. G., Gootenberg, J. S., Abudayyeh, O. O., & Zhang, F. (2019). SHERLOCK: nucleic acid detection with CRISPR nucleases. Nature Protocols, 14(10), 2986 – 3012.
- Kiatpathomchai W., Jaroenram W., Arunrut N., Jitrapakdee S., Flegel TW., Shrimp Taura syndrome virus detection by reverse transcription loop-mediated isothermal amplification combined with a lateral flow dipstick. Journal of Virological Methods 2008; 153: 214 – 217
- Kiatpathomchai, W., Jaroenram, W., Arunrut, N., Jitrapakdee, S., & Flegel, T. W. (2008). Shrimp Taura syndrome virus detection by reverse transcription loop-mediated isothermal amplification combined with a lateral flow dipstick. Journal of Virological Methods, 153(2), 214 – 217.
- Kikuchi, T., Aikawa, T., Oeda, Y., Karim, N., & Kanzaki, N. (2009). A rapid and precise diagnostic method for detecting the pinewood nematode Bursaphelenchus xylophilus by loop-mediated isothermal amplification. Phytopathology, 99(12), 1365 – 1369.
- Kusumawati A. et al., Use of reverse transcription loop-mediated isothermal amplification combined with lateral flow dipstick for an easy and rapid detection of Jembrana disease virus. Virusdisease. 2015 Sep;26(3):189 – 95
- Lalle M. et al., Loop-Mediated Isothermal Amplification-Lateral-Flow Dipstick (LAMP-LFD) to detect Toxoplasma gondii oocyst in ready-to-eat salad. Food Microbiol. 2018 Apr;70:137 – 142
- Ledlod, S., Bunroddith, K., Areekit, S., Santiwatanakul, S., & Chansiri, K. (2019). Development of a duplex lateral flow dipstick test for the detection and differentiation of Listeria spp. and Listeria monocytogenes in meat products based on loop-mediated isothermal amplification. Journal of Chromatography B: Analytical Technologies in the Biomedical and Life Sciences, 1139(October 2019), 121834.
- Li, T.-T., Wang, J.-L., Zhang, N.-Z., Li, W.-H., Yan, H.-B., Li, L., … Fu, B.-Q. (2019). Rapid and Visual Detection of Trichinella Spp. Using a Lateral Flow Strip-Based Recombinase Polymerase Amplification (LF-RPA) Assay. Frontiers in Cellular and Infection Microbiology, 9.
- Ma, Q., Yao, J., Yuan, S., Liu, H., Wei, N., Zhang, J., & Shan, W. (2019). Development of a lateral flow recombinase polymerase amplification assay for rapid and visual detection of Cryptococcus neoformans/C. gattii in cerebral spinal fluid. BMC Infectious Diseases, 19(1).
- Mamba T. S. et al., Lateral Flow Loop-Mediated Isothermal Amplification Test with Stem Primers: Detection of Cryptosporidium Species in Kenyan Children Presenting with Diarrhea. J Trop Med. 2018 Feb 26;2018:7659730
- Nair G. et al., Detection of Entamoeba histolytica by Recombinase Polymerase Amplification. Am. J. Trop. Med. 2015; 93 (3) 591 – 595
- Pecchia S. and Da Lio D., Development of a rapid PCR-Nucleic Acid Lateral Flow Immunoassay (PCR-NALFIA) based on rDNA IGS sequence analysis for the detection of Macrophomina phaseolina in soil.
- Peng, Y., Zheng, X., Kan, B., Li, W., Zhang, W., Jiang, T., … Qin, A. (2019). Rapid detection of Burkholderia pseudomallei with a lateral flow recombinase polymerase amplification assay. PLOS ONE, 14(7), e0213416.
- Piepenburg O., Williams C.H., Stemple D.L., Armes N.A., DNA Detectin Using Recombination Proteins
- Qi Y. et al., Development of a rapid and visual detection method for Rickettsia rickettsii combining recombinase polymerase assay with lateral flow test. PLoS One. 2018 Nov 26;13(11)
- Qi Y. et al., Development of a rapid and visual nucleotide detection method for a Chinese epidemic strain of Orientia tsutsugamushi based on recombinase polymerase amplification assay and lateral flow test. Int J Infect Dis. 2018 May;70:42 – 50.
- Rigano AL et al., Rapid and sensitive detection of Candidatus Liberibacter asiaticus by loop mediated isothermal amplification combined with a lateral flow dipstick BMC Microbiol. 2014; 1186
- Rohrman B. et al., Inhibiotion of Recombinase Polymerase Amplification by Background DNA: A Lateral Flow-Based Method for Enriching Target DNA. Anal. Chem. 2015 87 (3), 1963 – 1967
- Rosser et al., Isothermal Recombinase Polymerase Amplification (RPA) of Schistosoma haematobium DNA and Oligochromatographic Lateral Flow Detection. Parasit Vectors 2015; 8
- SUN, Y.-L., YEN, C.-H., & TU, C.-F. (2013). Visual Detection of Canine Parvovirus Based on Loop-Mediated Isothermal Amplification Combined with Enzyme-Linked Immunosorbent Assay and with Lateral Flow Dipstick. Journal of Veterinary Medical Science, 76(4), 509 – 516
- Saldarriaga O. A. et al., An Innovative Field-Applicable Molecular Test to Diagnose Cutaneous Leishmania Viannis ssp. Infections. PLoS Negl. Trop. Dis. 2016; 10 (4)
- Soliman H. and El-Matoubli M., Rapid detection and differentiation of carp oedema virus and cyprinid herpes virus-3 in koi and common carp. J Fish Dis. 2018 May;41(5):761 – 772.
- Soliman H. et al., Recombinase polymerase amplification assay combined with a lateral flow dipstick for rapid detection of Tetracapsuloides bryosalmonae, the causative agent of proliferative kidney disease in salmonids. Parasit Vectors. 2018 Apr 11;11(1):234.
- Sun YL et al., Visual Detection of Canine Parvovirus Based on Loop-Mediated Isothermal Amplification Combined with Enzyme-Linked Immunosorbent Assay and with Lateral Flow Dipstick. J. Vet. Med. Sci. 2014
- Surasilp T., Longyant S., Rukpratanporn S., Sridulyakul P., Sithigorngul P., Chaivisuthangkura P., Rapid and sensitive detection of Vibrio vulnificus by loop-mediated isothermal amplification combined with lateral flow dipstick targeted to rpoS gene. Molecular and Cellular Probes 2011; 25: 158 – 163
- Taboada L. et al., A new method for the rapid detection of Atlantic cod (Gadus morhua), Pacific cod (Gadus macrocephalus), Alaska pollock (Gadus chalcogrammus) and ling (Molva molva) using a lateral flow dipstick assay. Food Chem. 2017; 233, 182 – 189
- Vaagt F et al. Loop-mediated isothermal amplification (LAMP)-based method for rapid mushroom species identification. J. Agric. Food Chem. 2013; 61 (8), 1833 – 1840.
- Wang H. et al., Development and evaluation of serotype-specific recombinase polymerase amplification combined with lateral flow dipstick assays for the diagnosis of foot-and-mouth disease virus serotype A, O and Asia1. BMC Vet Res. 2018 Nov 20;14(1)
- Wang H. et al., Rapid visual detection of cyprinid herpesvirus 2 by recombinase polymerase amplification combined with a lateral flow dipstick. J Fish Dis. 2018 May 28
- Wong YL et al., Rapid authentication of Cordyceps by lateral flow dipstick. J. Pharm. Biomed. Anal 2015, 111, 306 – 310
- Wu, L., Ye, L., Wang, Z., Cui, Y., & Wang, J. (2019). Utilization of recombinase polymerase amplification combined with a lateral flow strip for detection of Perkinsus beihaiensis in the oyster Crassostrea hongkongensis. Parasites & Vectors, 12(1), 360.
- Yang Y et al., Development of an Isothermal Amplification-Based Assay for Rapid Visual Detection of an Orf Virus. Virol. J. 2016; 13:46
- Yang Y et al., Rapid and sensitive detection of Babesia bovis and Babesia bigemina by loop-mediated isothermal amplification combined with a lateral flow dipstick Vet. Parasitol 2016; 219, 71 – 76
- Zaky, W. I., Tomaino, F. R., Pilotte, N., Laney, S. J., & Williams, S. A. (2018). Backpack PCR: A point-of-collection diagnostic platform for the rapid detection of Brugia parasites in mosquitoes. PLoS Neglected Tropical Diseases, 12(11), 1 – 17.
- Zasada A. A. et al., Isothermal DNA amplification combined with lateral flow dipsticks for detection of biothreat agents. Anal Biochem. 2018 Nov 1;560:60 – 66.
- Zhao G et al. Development of a recombinase polymerase amplification combined with a lateral flow dipstick assay for rapid detection of the Mycoplasma bovis. BMC Vet Res. 2018 Dec 20;14(1):412
- Zhao G. et al., Rapid visual detection of Mycobacterium avium subsp. paratuberculosis by recombinase polymerase amplification combined with a lateral flow dipstick. J Vet Sci. 2018 Mar 31;19(2):242 – 250.