HybriDetect - Universal Lateral Flow Assay Kit
205.00 €
(Price excludes VAT and shipping costs)
100 tests
The HybriDetect is a simple and quick tool to develop your own rapid test. Various molecules can be detected: Proteins, Antibodies, Genetic amplicons
Overview
Name of Product
HybriDetect — Universal Lateral Flow Assay Kit
Catalog Number
MGHD 1
Short Info
The HybriDetect is a simple and quick tool to develop your own rapid test. Various molecules can be detected: Proteins, Antibodies, Genetic amplicons
Method/Patform
Lateral flow, sandwich or competetive
Range/Assay Sensivity
5 pg DNA, eqivalent to agarose gel electrophoresis
Test Principle
HybriDetect is a ready-to-use, universal test strip (dipstick) based on lateral flow technology using gold particles. The dipstick can be used to develop qualitative or quantitative rapid test systems for a wide range of analytes such as antibodies, gene amplification products or proteins . The results can be interpreted qualitative or quantitative.
Protocol
Brief Instructions
Storage
2 – 8°C
Components
HybriDetect dipsticks, HybriDetect assay buffer
References
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Videos
FAQs
Q:
Can I use FAM instead of FITC as a label of the probes or primers?
A:
Yes, but initially negative strips may show a faint band after prolonged time, while positive bands will not disappear! We recommend to take an image of the strip shortly after the incubation time is over.
Q:
What is the difference between HybriDetect and HybriDetect 2T?
A:
HybriDetect allows the detection of 1 target on the strip, while HybriDetect 2T allows the detection of 2 targets on the same strip.
Q:
What is the sensitivity of HybriDetect compared to an agarose gel?
A:
This depends on the size of the amplicon. The shorter the amplicon is, the lower is the sensitivity of the agarose gel. If the amplicon is 100 bases long HybriDetect could possibly be 1.000 fold more sensitive compared to the agarose gel.
Q:
Can I still interpret the results of the strips after a prolonged time, e.g. 1 day?
A:
Yes, but initially negative strips may show a faint band after prolonged time, while positive bands will not disappear! We recommend to take an image of the strip shortly after the incubation time is over.
Q:
Do you accept credit card for payment?
A:
Yes, we accept credit card for payment if you purchase our products online via our webshop.
Q:
Do you have a MSDS available for the HybriDetect products?
A:
Yes, you can download the pdf-file of the MSDS for HybriDetect here here and for HybriDetect 2T here.
Q:
How long does it take from the time of my order to shipping?
A:
The products are in inventory and we ship the same day if we receive the order prior 12.00 CET.
Q:
Is it necessary that the kits are shipped at 2 – 8°C?
A:
No, kits can be shipped at ambient temperatures, but should be stored at 2 – 8°C after receipt.
Q:
I received my kits 3 weeks after you shipped them. Do they still perform well?
A:
Yes, the kits will still perform well. We have made stability tests and could demonstrate that the kits still perform well after storing them for 100 days at 37°C.
Q:
What should be the volume of the sample and buffer I am using in order to run HybriDetect?
A:
The sum of the volume of the sample and the buffer should not exceed 100 µl.
Q:
I am doing an amplification and see that all of my samples are giving positive results. What is the reason for this?
A:
This phenomenon is due to the formation of primer dimers. In this event biotinylated and FITC-labeled primers bind to each other and form dimers resulting in false positive results. You need to redesign your primers in order to prevent primer dimerization.
Q:
What is the shelf life of HybriDetect kits from the time of production?
A:
The shelf life of the kits from the time of production is 24 months. Milenia Biotec is manufacturing fresh lots twice a year, meaning that our customers usually get kits with a remaining shelf life of not less than 12 months.
Q:
Are there any compounds known which may result in an interference with the test or its performance?
A:
Yes, in case the concentration of labeled primers or probes is too high, this may lead to a decreased sensitivity of the test. In this case primer or probe concentration need to be reduced. Furthermore, components of the sample or the sample buffer, including SDS, DTT or urea may have a negative impact on the performance of the test. In this case the sample can be diluted in order to „dilute the effect out“. However this approach will result in a reduced sensitivity of the test. It is also possible to develop a modified assay buffer to reduce the interferences.
Q:
Can HybriDetect be used at elevated temperatures, like hybridizing conditions?
A:
Yes, we demonstrated that HybriDetect (MGHD 1) still performs well at temperatures up to 75°C and HybriDetect 2T at temperatures up to 65°C.
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