
HybriDetect - Universal Lateral Flow Assay Kit
The HybriDetect is a simple and quick tool to develop your own rapid test. Various molecules can be detected: Proteins, Antibodies, Genetic amplicons
The HybriDetect is a simple and quick tool to develop your own rapid test. Various molecules can be detected: Proteins, Antibodies, Genetic amplicons
HybriDetect is a ready-to-use, universal test strip (dipstick) based on lateral flow technology using gold particles. The dipstick can be used to develop qualitative or quantitative rapid test systems for a wide range of analytes such as antibodies, gene amplification products or proteins . The results can be interpreted qualitative or quantitative.

2-8°C
HybriDetect dipsticks, HybriDetect assay buffer
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A CRISPR-based assay for the detection of opportunistic infections post-transplantation and for the monitoring of transplant rejection. (2020)
Kaminski M., Alcantar M., Lape I., et.al.
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A new method for the rapid detection of Atlantic cod (Gadus morhua), Pacific cod (Gadus macrocephalus), Alaska pollock (Gadus chalcogrammus) and ling (Molva molva) using a lateral flow dipstick assay. Food Chem. 2017; 233, 182-189 (2017)
Taboada L. et al.
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A Novel Test to Diagnose Canine Visceral Leishmaniasis at the Point of Care. Am. J. Med Hyg. 2015, 93 (5), 970-975 (2015)
Castellanos-Gonzalez A et al.
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A Paper and Plasitc Device for the Combined Isothermal Amplification and Lateral Flow Detection of Plasmodium DNA. Malar. J. 2015; 14, 472 (2015)
Cordray MS et al.
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A protocol for detection of COVID-19 using CRISPR diagnostics. (2020)
Zhang F., Abudayyeh O. O., Gootenberg J.S., Mathers L.
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A protocol for rapid detection of the 2019 novel coronavirus SARS-CoV-2 using CRISPR diagnostics : SARS-CoV-2 DETECTR. (2020)
Broughton J. P., Wayne D., Fasching C. L., Singh J., Charles Y., Chen J. S.
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A rapid and precise diagnostic method for detecting the pinewood nematode Bursaphelenchus xylophilus by loop-mediated isothermal amplification. Phytopathology, 99(12), 1365–1369. (2009)
Kikuchi, T., Aikawa, T., Oeda, Y., Karim, N., & Kanzaki, N.
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A rapid, equipment-free method for detecting Phytophthora infestans in the field using a lateral flow strip-based recombinase polymerase amplification assay. (2020)
Xinyu L., Zheng Y., Zhang F.
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A recombinase polymerase amplification lateral flow dipstick assay for rapid detection of the quarantine citrus pathogen in China, Phytophthora hibernalis (2019)
Dai T, Hu T, Yang X, Shen D, Jiao B, Tian W, Xu Y
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A recombinase polymerase amplification lateral flow dipstick for field diagnosis of bovine leukemia virus infection and its effectiveness compared to iiPCR and ELISA (2018)
Tu PA, Shiu JS, Lai FY, Chen YH, Shiau JW, Pang VF, Wang PH
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An enhanced isothermal amplification assay for viral detection. (2020)
Qian J., Boswell S., Chidley C., et. al.
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An enhanced isothermal amplification assay for viral detection. (2020)
Qian J. et al
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An Innovative Field-Applicable Molecular Test to Diagnose Cutaneous Leishmania Viannis ssp. Infections. PLoS Negl. Trop. Dis. 2016; 10 (4) (2016)
Saldarriaga O. A. et al.
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An RxLR Effector Gene and New Biomarker in A Recombinase Polymerase Amplification Assay for Rapid Detection of Phytophthora cinnamomi. (2020)
Dai T., Wang A., Yang X., Yu X., Tain W., Xu Y
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Application of loop-mediated isothermal amplification combined with colorimetric and lateral flow dipstick visualization as the potential point-of-care testing for Corynebacterium diphtheria. (2020)
Zasada A., Wiatrzyk A., Czajka U., et. al.
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Backpack PCR: A point-of-collection diagnostic platform for the rapid detection of Brugia parasites in mosquitoes (2018)
Zaky WI, Tomaino FR, Pilotte N, Laney SJ, Williams SA
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Cas12a-based on-site and rapid nucleic acid detection of african swine fever (2019)
Bai J, Lin H, Li H, Zhou Y, Liu J, Zhong G, Wu L, Jiang W, Du H, Yang J, Xie Q, Huang L
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Comparative evaluation of a novel recombinase polymerase amplification-lateral flow dipstick (RPA-LFD) assay, LAMP, conventional PCR, and leaf-disc baiting methods for detection of Phytophthora sojae (2019)
Dai T, Yang X, Hu T, Jiao B, Xu Y, Zheng X, Shen D
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COVID-19 infection diagnosis: Potential impact of isothermal amplification technology to reduce community transmission of SARS-CoV-2. (2020)
James A., Alwneh J.,
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CRISPR-based platform for carbapenemases and emerging viruses detection using Cas12a (Cpf1) effector nuclease. (2020)
Curti L., Pereyra-Bonnet F., Repizo G., et. al.
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CRISPR-based surveillance for COVID-19 using genomically-comprehensive machine learning design. (2020)
Metsky H. C., Freije C. A., Kosoko-Thoroddsen T-S. F., Sabeti P.C., Myhrvold C.,
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CRISPR-Cas fluorescent cleavage assay coupled with recombinase polymerase amplification for sensitive and specific detection of Enterocytozoon hepatopenaei. (2020)
Kanitchinda S., Srisala J., Suebsing R., et. al.
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CRISPR-Cas-amplified urine biomarkers for multiplexed and portable cancer diagnostics (2020)
Hao L., Zhao R., Ngambenjawong C., et. al.
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CRISPR-Cas12-based detection of SARS-CoV-2, Nature Biotechnology (2020)
Broughton J.P., Deng X., Yu G., Fasching C.L., Servellita V., Singh J., Miao X., Streithorst J.A., Granados A., Sotomayor-Gonzales A., Zorn K., Gopez A., Hsu E., Gu W., Miller S., Pan C.Q., Guevara H., Wadford D.A., Chen J.S., Chiu C.Y.
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CRISPR-Cas12-based detection of SARS-CoV-2. (2020)
Broghton J.P., et al.
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Detection assay for HPV16 and HPV18 by loop‑mediated isothermal amplification with lateral flow dipstick tests (2017)
Kumvongpin R, Jearanaikoon P, Wilailuckana C, Sae‑Ung N, Prasongdee P, Daduang S, Wongsena M, Boonsiri P, Kiatpathomchai W, Swangvaree SS, Sandee A, Daduang J
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Detection of Entamoeba histolytica by Recombinase Polymerase Amplification. Am. J. Trop. Med. 2015; 93 (3) 591-595 (2015)
Nair G. et al.
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Detection of Helminth Ova in Wastewater Using Recombinase Polymerase Amplification Coupled to Lateral Flow Strips. (2020)
Ravindran V. B., Khallaf B., Surapaneni A., Crosbie N.D., Soni S.K., Ball A.S.
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Detection of Mycobacterium tuberculosis by using loop-mediated isothermal amplification combined with a lateral flow dipstick in clinical samples. Biomed. Res. Int. 2013; 1-6 (2013)
Kaewphinit T., Arunrut N., Kiatpathomchai W., Santiwatanakul S., Jaratsing P., Chansiri K.
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Detection of Skeletonema costatum based on loop-mediated isothermal amplification combined with lateral flow dipstick. Mol Cell Probes. 2017 Dec;36:36-42. (2017)
Huang H. L. et al.
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Development and evaluation of serotype-specific recombinase polymerase amplification combined with lateral flow dipstick assays for the diagnosis of foot-and-mouth disease virus serotype A, O and Asia1 (2018)
Wang H, Hou P, Zhao G, Yu L, Gao YW, He H
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Development of a duplex lateral flow dipstick test for the detection and differentiation of Listeria spp. and Listeria monocytogenes in meat products based on loop-mediated isothermal amplification (2019)
Ledloda S, Bunroddithd K, Areekit S, Santiwatanakulc S, Chansiria K
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Development of a lateral flow recombinase polymerase amplification assay for rapid and visual detection of Cryptococcus neoformans/C. gattii in cerebral spinal fluid (2019)
Ma Q, Yao J, Yuan S, Liu H, Wei N, Zhang J, Shan W
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Development of a rapid and visual detection method for Rickettsia rickettsii combining recombinase polymerase assay with lateral flow test (2018)
Qi Y, Shao Y, Rao J, Shen W, Yin Q, Li X, Chen H, Li J, Zeng W, Zheng S, Liu S, Li Y
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Development of a rapid and visual nucleotide detection method for a Chinese epidemic strain of Orientia tsutsugamushi based on recombinase polymerase amplification assay and lateral flow test (2018)
Qia Y, Yin Q, Shaob Y, Caoa M, Lia S, Chena H, Shena W, Raoa J, Lia J, Lib X, Sunc Y, Linb Y, Dengb Y, Zengb W, Zhengb S, Liub S, Lia Y
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Development of a rapid PCR-nucleic acid lateral flow immunoassay (PCR-NALFIA) based on rDNA IGS sequence analysis for the detection of Macrophomina phaseolina in soil (2018)
Pecchia S, Da Lio D
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Development of a recombinase polymerase amplification combined with a lateral flow dipstick assay for rapid detection of the Mycoplasma bovis (2018)
Zhao G, Hou P, Huan Y, He C, Wang H, He H
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Development of a recombinase polymerase amplification lateral flow assay for the detection of active Trypanosoma evansi infections. (2020)
Li Z., Torres J., Goossens J., et. al.
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Development of an Isothermal Amplification-Based Assay for Rapid Visual Detection of an Orf Virus. Virol. J. 2016; 13:46 (2016)
Yang Y et al.
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Development of Recombinase Polymerase Amplification Assays for Detection of Orienta Tsutsugamushi or Rickettsia typhi. PLoS Negl. Trop. Dis. 2015; 9 (7) (2015)
Chao CC et al.
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Development of the Rapid Test Kit for the Identification of Campylobacter spp. Based on Loop-mediated Isothermal Amplification (LAMP) in Combination with a Lateral Flow Dipstick (LFD) and Gold Nano-DNA Probe (AuNPs) (2019)
Thongphueak D, Chansiri K, Sriyapai T , Areekit S, Santiwatanakul S, Wangroongsarb P
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DNA Detectin Using Recombination Proteins (2006)
Piepenburg O., Williams C.H., Stemple D.L., Armes N.A.
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Enhancement of trans-cleavage activity of Cas12a with engineered crRNA enables amplified nucleic acid detection. (2020)
Nguyen L., Smith B., Jain P
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Enhancement of trans-cleavage activity of Cas12a with engineered crRNA enables amplified nucleic acid detection. (2020)
Nguyen L., Smith B., Jain P.
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Evaluation of CRISPR/Cas12a-based DNA detection for fast pathogen diagnosis and GMO test in rice. (2020)
Zhang Y., Xie K.,
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Genome-informed diagnostics for specific and rapid detection of Pectobacterium species using recombinase polymerase amplification coupled with a lateral flow device (2018)
Ahmed FA, Larrea-Sarmiento A, Alvarez AM, Arif M
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Inhibiotion of Recombinase Polymerase Amplification by Background DNA: A Lateral Flow-Based Method for Enriching Target DNA. Anal. Chem. 2015 87 (3), 1963-1967 (2015)
Rohrman B. et al.
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Isothermal DNA amplification combined with lateral flow dipsticks for detection of biothreat agents. Anal Biochem. 2018 Nov 1;560:60-66. (2018)
Zasada A. A. et al.
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Isothermal Recombinase Polymerase Amplification (RPA) of Schistosoma haematobium DNA and Oligochromatographic Lateral Flow Detection. Parasit Vectors 2015; 8 (2015)
Rosser et al.
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Lateral Flow Loop-Mediated Isothermal Amplification Test with Stem Primers: Detection of Cryptosporidium Species in Kenyan Children Presenting with Diarrhea. J Trop Med. 2018 Feb 26;2018:7659730 (2018)
Mamba T. S. et al.
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Loop-mediated isothermal amplification (LAMP)-based method for rapid mushroom species identification. J. Agric. Food Chem. 2013; 61 (8), 1833-1840. (2013)
Vaagt F et al.
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Loop-Mediated Isothermal Amplification-Lateral-Flow Dipstick (LAMP-LFD) to detect Toxoplasma gondii oocyst in ready-to-eat salad (2018)
Lalle M, Possenti A, P. Dubey JP, Pozio E
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Molecular method for rapid detection of the red tide dinoflagellate Karenia mikimotoi in the coastal region of Xiangshan Bay, China. (2020)
Huang H. L., Gao W.F., Zhu P., Zhou C.X., Qiao L.L., Dang C.Y., Pang J.H., Yan X.J.,
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Nucleic acid detection of plant genes using CRISPR-Cas13 (2019)
Abudayyeh OO, Gootenberg JS, Kellner MJ, Zhang F
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Nucleic Acid Detection Using CRISPR/Cas Biosensing Technologies. (2020)
Aman R., Mahas A., Mahfouz M.,
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Optimization of the Isothermal Amplification Method for Mycobacterium Tuberculosis Detection and Visualization for Fieldwork. (2020)
Ağel H.E., Sağcan H., Ceyhan I., Durmaz R.
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Point-of-care testing for COVID-19 using SHERLOCK diagnostics. (2020)
Joung J., Ladha A., Saito M., et. al.
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Rapid analysis of Escherichia coli O157:H7 using isothermal recombinase polymerase amplification combined with triple-labeled nucleotide probes. (2020)
Hu J., Wang Y., Su H., et. al.
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Rapid and accurate detection of novel coronavirusSARS-CoV-2 using CRISPR-Cas3. (2020)
Yoshimi K., Takeshita K., Yamayoshi S., et. al.
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Rapid and accurate species identification for ecological studies and monitoring using CRISPR-based SHERLOCK. (2020)
Baerwald M., Goodbla A., nagarajan R., Gootenberg J., Abudayyeh O., Zhang F., Schreier A.
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Rapid and sensitive detection of Babesia bovis and Babesia bigemina by loop-mediated isothermal amplification combined with a lateral flow dipstick Vet. Parasitol 2016; 219, 71-76 (2016)
Yang Y et al.
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Rapid and sensitive detection of Candidatus Liberibacter asiaticus by loop mediated isothermal amplification combined with a lateral flow dipstick BMC Microbiol. 2014; 1186 (2014)
Rigano AL et al.
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Rapid and sensitive detection of infectious spleen and kidney necrosis virus by loop-mediated isothermal amplification combined with a lateral flow dipstick (2010)
W.C. Ding, Jiong Chen, Y.H. Shi, X.J. Lu, M.Y. Li
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Rapid and sensitive detection of infectious spleen and kidney necrosis virus by recombinase polymerase amplification combined with lateral flow dipsticks. (2020)
Li H., Yuan G., Luo Y., Yu Y., Ai T., Su J.
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Rapid and simple detection of Glaesserella parasuis in synovial fluid by recombinase polymerase amplification and lateral flow strip (2019)
Zhang TT, Liu MZ, Yin RH, Yao LQ, Liu BS, Chen ZL
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Rapid and visual detection of enterovirus using recombinase polymerase amplification combined with lateral flow strips. (2020)
Yang X., Xie J., Hu S., Zhan W., Duan L., Chen K., Zhang C., Yin A., Luo M
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Rapid and visual detection of porcine deltacoronavirus by recombinase polymerase amplification combined with a lateral flow dipstick (2020)
Gao X., Liu X., Zhang Y., et. al.
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Rapid and visual detection of Trichinella spp. using a lateral flow strip-based recombinase polymerase amplification (LF-RPA) assay (2019)
Li TT, Wang JL, Zhang NZ, Li WH, Yan HB, Li L,Jia WZ, Fu BQ
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Rapid and visual detection of Verticillium dahliae using recombinase polymerase amplification combined with lateral flow dipstick. (2020)
Ju Y., Li C., Shen P., et. al.,
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Rapid authentication of Cordyceps by lateral flow dipstick. J. Pharm. Biomed. Anal 2015, 111, 306-310 (2015)
Wong YL et al.
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Rapid detection and differentiation of carp oedema virus and cyprinid herpes virus-3 in koi and common carp. J Fish Dis. 2018 May;41(5):761-772. (2018)
Soliman H. and El-Matoubli M.
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Rapid Detection of 2019 Novel Coronavirus SARS-CoV-2 Using a CRISPR-based DETECTR Lateral Flow Assay. (2020)
Broughton J. P., Deng X., Fasching C.L., Singh J., Streithorst J., Granados, A., Sotomayor-Gonzalez A., Zorn K., Gopez A., Hsu E., Gu W., Miller S., Pan C., Guevara H., Wadford D., Chen J., Chio C.Y.
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Rapid detection of African swine fever virus using Cas12a-based portable paper diagnostics. (2020)
Lu L., Li F., Chen Q., et. al
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Rapid detection of bovine viral diarrhea virus using recombinase polymerase amplification combined with lateral flow dipstick assays in bulk milk (2018)
Hou P, Zhao G, Wang H, He C, He H
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Rapid detection of Burkholderia pseudomallei with a lateral flow recombinase polymerase amplification assay (2019)
Peng Y, Zheng X, Kann B, Li W, Zhang W, Jiang T, Lu J, Qin A
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Rapid diagnosis of two marine viruses, red sea bream iridovirus and viral hemorrhagic septicemia virus by PCR combined with lateral flow assay. (2020)
Seo H., Kil E., Fadhila C., et. al.
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Rapid SARS-CoV-2 testing in primary material based on a novel multiplex LAMP assay (2020)
Schermer B., Fabretti F., Damagnez M., et. al.
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Rapid visual detection of cyprinid herpesvirus 2 by recombinase polymerase amplification combined with a lateral flow dipstick (2018)
Wang H, Sun M, Xu D, Podok P, Xie J, Jiang Y, Lu L
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Rapid visual detection of Mycobacterium avium subsp. paratuberculosis by recombinase polymerase amplification combined with a lateral flow dipstick (2018)
Zhao G, Wang H, Hou P, He C, He H
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Rapid, CRISPR-based, field-deployable detection of white spot syndrome virus in shrimp (2019)
Sullivan TJ, Arun KD, Cruz-Flores R, Bodnar AG
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Recombinase Polymerase Amplification and Lateral Flow Assay for Ultrasensitive Detection of Low-Density. (2020)
Lalremruata A., Nguyen T., MCCall M., et. al.
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Recombinase polymerase amplification assay combined with a lateral flow dipstick for rapid detection of Tetracapsuloides bryosalmonae, the causative agent of proliferative kidney disease in salmonids. Parasit Vectors. 2018 Apr 11;11(1):234. (2018)
Soliman H. et al.
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Recombinase polymerase amplification-lateral flow (RPA-LF) assay combined with immunomagnetic separation for rapid visual detection of Vibrio parahaemolyticus in raw oysters. (2020)
Jiang W., Ren Y., Han X., Xue J., Shan T., Chen Z., Liu Y., Quan W.
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SHERLOCK: nucleic acid detection with CRISPR nucleases (2019)
Kellner MJ, Koob JG, Gootenberg JS, Abudayyeh OO, Zhang F
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Supporting Information Clustered Regularly Interspaced Short Palindromic Repeats / Cas9- Mediated Lateral Flow Nucleic Acid Assay. (2020)
Wang X., Xion E., Tian T., Cheng M., Lin W., Wang H.
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The development of highly specific and sensitive primers for the detection of potentially allergenic soybean (Glycine max) using loop-mediated isothermal amplification combined with lateral flow dipstick (LAMP-LFD). (2020)
Allgöwer S., Hartmann C., Holzhauser T.
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The Development of Loop-Mediated Isothermal Amplification Combined with Lateral Flow Dipstick for Detection of Karlodinium veneficum. Harmful Algae 2017; 62, 20-29. (2017)
Huang HL et al.
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Time course of detection of human male DNA from stained blood sample on various surfaces by Loop Mediated Isothermal Amplification and Polymerase Chain Reaction (2018)
Kanchanaphum P
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Trends and Innovations in Biosensors for COVID-19 Mass Testing (2020)
Santiago I.
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Use of a recombinase polymerase amplification commercial kit for rapid visual detection of Pasteurella multocida (2019)
Zhao G, He H, Wang H
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Use of reverse transcription loop-mediated isothermal amplification combined with lateral flow dipstick for an easy and rapid detection of Jembrana disease virus. Virusdisease. 2015 Sep; 26(3):189-95 (2015)
Kusumawati A. et al.
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Utilization of recombinase polymerase amplification combined with a lateral flow strip for detection of Perkinsus beihaiensis in the oyster Crassostrea hongkongensis (2019)
Wu L, Ye L, Wang Z, Cui Y, Wang J
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Visual Detection of Canine Parvovirus Based on Loop-Mediated Isothermal Amplification Combined with Enzyme-Linked Immunosorbent Assay and with Lateral Flow Dipstick. J. Vet. Med. Sci. 2014 (2014)
Sun YL et al.
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Visual detection of porcine reproductive and respiratory syndrome virus using CRISPR-Cas13a. (2020)
Chang Y., Deng Y., Li T.
Yes, but initially negative strips may show a faint band after prolonged time, while positive bands will not disappear! We recommend to take an image of the strip shortly after the incubation time is over.
HybriDetect allows the detection of 1 target on the strip, while HybriDetect 2T allows the detection of 2 targets on the same strip.
This depends on the size of the amplicon. The shorter the amplicon is, the lower is the sensitivity of the agarose gel. If the amplicon is 100 bases long HybriDetect could possibly be 1.000 fold more sensitive compared to the agarose gel.
Yes, but initially negative strips may show a faint band after prolonged time, while positive bands will not disappear! We recommend to take an image of the strip shortly after the incubation time is over.
Yes, we accept credit card for payment if you purchase our products online via our webshop.
Yes, you can download the pdf-file of the MSDS for HybriDetect here here and for HybriDetect 2T here.
The products are in inventory and we ship the same day if we receive the order prior 12.00 CET.
No, kits can be shipped at ambient temperatures, but should be stored at 2 – 8°C after receipt.
Yes, the kits will still perform well. We have made stability tests and could demonstrate that the kits still perform well after storing them for 100 days at 37°C.
The sum of the volume of the sample and the buffer should not exceed 100 µl.
This phenomenon is due to the formation of primer dimers. In this event biotinylated and FITC-labeled primers bind to each other and form dimers resulting in false positive results. You need to redesign your primers in order to prevent primer dimerization.
The shelf life of the kits from the time of production is 24 months. Milenia Biotec is manufacturing fresh lots twice a year, meaning that our customers usually get kits with a remaining shelf life of not less than 12 months.
Yes, in case the concentration of labeled primers or probes is too high, this may lead to a decreased sensitivity of the test. In this case primer or probe concentration need to be reduced. Furthermore, components of the sample or the sample buffer, including SDS, DTT or urea may have a negative impact on the performance of the test. In this case the sample can be diluted in order to „dilute the effect out“. However this approach will result in a reduced sensitivity of the test. It is also possible to develop a modified assay buffer to reduce the interferences.
Yes, we demonstrated that HybriDetect (MGHD 1) still performs well at temperatures up to 75°C and HybriDetect 2T at temperatures up to 65°C.