
HybriDetect RPA basic Kit
Fast, precise isothermal DNA amplification at 37–42 °C without thermocycler. This ready-to-use, lyophilized format delivers highly sensitive molecular results in under 20 minutes. It is ideal for multiple applications in molecular biology research and field-based point-of-need testing.
Combined with our lateral flow detection kits (HybriDetect, HybriDetect 2T, HybriDetect Cassette), RPA provides a fast and easy solution from DNA amplification to visual readout.
The kit contains the following components:
- 96 x cake LyoBead RPA basic
- 3 x 1.0 ml 2X RPA Reconstitution Buffer X
- 3 x 0.1 ml 20X RPA Reaction Initiator
- 96 x caps Caps for LyoBead
Fast, precise isothermal DNA amplification at 37–42 °C without thermocycler. This ready-to-use, lyophilized format delivers highly sensitive molecular results in under 20 minutes. It is ideal for multiple applications in molecular biology research and field-based point-of-need testing.
Combined with our lateral flow detection kits (HybriDetect, HybriDetect 2T, HybriDetect Cassette), RPA provides a fast and easy solution from DNA amplification to visual readout.
The kit contains the following components:
- 96 x cake LyoBead RPA basic
- 3 x 1.0 ml 2X RPA Reconstitution Buffer X
- 3 x 0.1 ml 20X RPA Reaction Initiator
- 96 x caps Caps for LyoBead
The test principle of Recombinase Polymerase Amplification (RPA) is based on the rapid, highly efficient, and specific amplification of DNA under constant, typically low-temperature conditions (isothermal, usually at 37–42 °C). Unlike traditional polymerase chain reaction (PCR), it eliminates the need for thermal cycling because strand separation is initiated enzymatically by recombinases rather than heat, allowing continuous amplification at a constant temperature.

1. Prepare a 50 µl reaction
- LyoBead RPA basic cake
- 2X RPA Reconstitution Buffer X 25 µl
- Primers and Probes variable
- Template DNA variable
- Nuclease free water add up to 47.5 μl
Mix gently and transfer the complete volume of the mix to a reaction tube of your choice. Alternatively, keep the mix in the original tube and use the provided lid. Then add to the lid:
- 20X RPA Reaction Initiator 2.5 µl
Total volume 50 µl
2. Spin down the Reaction Initiator. The reaction starts immediately.
3. Incubate at 37-42°C for 10-30 min
4. Evaluation
- Agarose Gel Electrophoresis
- Lateral Flow Strip (see Instructions for Use for details)
2 – 30 °C
LyoBead RPA basic | 2X RPA Reconstitution Buffer X | 20X RPA Reaction Initiator I Caps for LyoBead







