Lateral Flow Test and Development Blog
06/15
2021
Milenia HybriDetect – Nucleic Acid Amplification and Lateral Flow
Part II – The 5 Most Relevant Amplification Methods
I have been working on universal lateral flow devices (especially nucleic acid amplification and lateral flow) for more than eight years. Since then, I try to get an overview of the variety of applications, test them and make them more accessible for simple and rapid lateral flow readout. After all these years, experiences and, of course, failures, the enthusiasm for this apparently simple platform is still unbroken. There are no limits for individual creativity, because there is so much charme in the simplicity of the lateral flow technology. There are countless ways to think up, modify and optimize the test strips and the associated methods. For me personally, Nucleic Acid Lateral Flow Immunoassays (NALFIAs) are the ultimate playground for creativity. And finally, innovative test systems with excellent precision and sensitivity can be created. The following article is intended to describe an important part of the “lateral flow-world”: the combination of nucleic acid amplification and lateral flow. read more…
03/15
2021
Milenia HybriDetect – A Universal Lateral Flow Development Platform
Part I – Lateral Flow Basics, Composition and Detection Strategies
Molecular detection methods in particular are indispensable in any diagnostic laboratory. At the same time, rapid test formats are moving into the focus of public awareness. Due to their simplicity, these rapid tests can be used as a point of need tool. The following article focuses on one of the most exciting topics in diagnostics today. It is about the connection of complex molecular biological detection techniques combined with the simplicity of universal lateral flow assays. The Milenia HybriDetect is a universal platform which allows individual and innovative Lateral Flow development. The first part of this series of articles deals with the most important basics: What is Lateral Flow? What is the Milenia HybriDetect and how is it possible to detect anything with it?
read more…
03/11
2021
Protein and Antibody Purification: Detection via Milenias Rapid Test ProteinDetect
The fast and easy Dotblot alternative
Our new tool for researchers, called ProteinDetect, has two main features: First ProteinDetect allows you to verify if your protein of interest is present in a random sample. Second you can use it vice versa and check if your antibody is specific against a know antigen. All within 10 minutes!
read more…
03/04
2021
Covid Mutation Test with CRISPR and HybriDetect
Rapid and simple identification of Covid mutants by a combination of a CRISPR based method with the lateral flow dipstick Milenia HybriDetect
At the end of 2019 the first infections of the coronavirus (SARS-CoV-2) in humans have been identified in Wuhan, China. Since then the covid 19 outbreak has been distributed over the entire world with millions of people infected and a lot of reported deaths.
read more…
02/15
2021
Plant Pathogens – Rapid Detection with a universal Lateral Flow Device
Lateral Flow meets Plant Pathology
We at Milenia Biotec care about simple rapid test development. Nevertheless, some of our colleagues originally come from plant research. That’s why we are particularly pleased when our heart-to-heart issue, lateral flow assays, meets the old love “Plant Science, Plant Pathology or Plant Health”. For this reason, we are adressing a topic with global significance — the detection of plant pathogens. First of all, the focus should be on two interesting open access papers describing the combination of different isothermal DNA-amplification techniques combined with a rapid and simple lateral flow readout. Both publications adress the same topic – the development of a potentially field applicable detection system for one the most destructive plant pathogens worldwide. read more…
01/26
2021
Lateral Flow Test and Development Blog – The Top Three Articles in 2020
The year 2020 was the first full year of blogging on our company blog, called the “Lateral Flow Test and Development Blog”. We made it to publish new articles on a regular basis and we received a lot of valuable comments on the published content from our customers and readers. On this occasion we wanted to thank you all who have contributed to the discussions.
We were very keen to find out which publications on the blog were the most frequently read articles in 2020. Here are our top 3: read more…
11/03
2020
Loop mediated isothermal Amplification and Lateral Flow
Tips and Tricks for a successful Assay Development
Isothermal Amplification of Nucleic Acids — A Brief Introduction
Isothermal amplification techniques have developed massively over the past decade. Point-of-Care (POC) applications in particular benefit from the advantages of these methods. As the name suggests, DNA synthesis takes place at constant temperature if isothermal amplification methods are applied. As a result, complex and expensive equipment such as thermal cyclers are not necessary for isothermal amplification reactions. A simple incubator, heating block, water bath, suit vide cooker, the armpit or the closed hand are sufficient to carry out the amplification reaction. read more…
10/26
2020
Lateral Flow Test Development for COVID-19 based on Nucleic Acid Detection
Amplicon detection via the lateral flow test Milenia “HybriDetect”
Recently Milenia Biotec GmbH received several customer inquiries related to COVID-19 detection, due to the fact, that HybriDetect may be used for the detection of RPA, LAMP or PCR amplicons. Following up these inquiries, we did a literature search on the SHERLOCK (Specific High Sensitivity Enzymatic Reporter UnLOCKing) technique, which is a powerful tool to detect genetic information coming from viruses using CRISPR. read more…
10/05
2020
Feluda Test – A new rapid test method for the identification of SARS-CoV-2
CRISPR-test gets approval in India
Just recently, the TATA Group received approval for the commercial launch of “Feluda” (acronym for FNCas9 Editor Limited Uniform Detection Assay) by the Drugs Controller Gerneral of India.
What is Feluda?
The Feluda test is the first CRISPR/Cas9 (Clustered Regularly Interspaced Short Palindromic Repeats) rapid test available for COVID-19 detection. With this new qPCR independent point-of-care diagnostic approach, the genomic sequence of SARS-CoV-2 can be detected by Milenias lateral flow assay HybriDetect. read more…
09/25
2020
How to overcome contamination in DNA amplification based Lateral Flow Assays
Contamination can happen, no matter what type of amplification method used: LAMP, RPA or PCR
Milenias HybriDetect is a ready-to-use, universal dipstick based on the lateral flow technology for the detection of various molecules. It is mainly used for the detection of amplicons, generated by LAMP, RPA or PCR by our customers.
As in standard PCR experiments, which are analyzed by gel electrophoresis or qPCR experiments, cross-contamination is a problem our customers are faced with. Therefore we want to give some information and advice to help you avoid contaminations in RPA, LAMP or PCR assays combined with our Lateral Flow tests HybriDetect and HybriDetect 2T. read more…