Devel­opment of a nucleic acid based COVID-​​19 Rapid Test

Amplicon detection via the lateral flow test Milenia “HybriDetect”

Recently Milenia Biotec GmbH received several customer inquiries related to COVID-​​19 detection, due to the fact, that HybriDetect may be used for the detection of RPA, LAMP or PCR amplicons. Following up these inquiries, we did a liter­ature search on the SHERLOCK (Specific High Sensi­tivity Enzymatic Reporter UnLOCKing) technique, which is a powerful tool to detect genetic infor­mation coming from viruses using CRISPR.

What is HybriDetect?

The HybriDetect is a lateral flow dipstick (LFD), which is capable to detect different molecules, including gene ampli­fi­cation products, proteins and antibodies. A commonly used appli­cation for our test is the detection of gene ampli­fi­cation products resulting from PCR, LAMP or RPA. The under­lying genetic infor­mation can be detected regardless which kind of organism need to be detected.Finally, labeled primers must be intro­duced in the ampli­fi­cation step, so that the resulting fragments are labeled and can be detected by the HybriDetect dipstick.

Results can be reported within 5 minutes after lines become visible on the test strip.


Improving the sensi­tivity of the HybriDetect 2T

How to use block oligos for better results

Detecting various PCR amplifi­cates with HybriDetect 2T

The ampli­fi­cation of nucleic acids has become an important tool for various areas such as medical diagnostics, food safety and research over the past decades. A quick and easy way to get a visible result of the ampli­fi­cation is to evaluate the ampli­fi­cation product using the HybriDetect 2T strip, a lateral flow evalu­ation strip.


Lateral Flow based post-​​transplantation Monitoring

CRISPR-​​based diagnosis of infection and rejection with the HybriDetect

HybriDetect helps diagnose viral infec­tions and post-​​transplantation Monitoring with SHERLOCK

Kaminski et al. developed a fast and easy test to detect causes of graft loss in blood and urine samples from post-​​transplant patients after kidney trans­plan­tation.

The need for a new method to detect organ rejec­tions is highly given, because current invasive methods can take several days and are connected with high costs. The loss of time can lead to an increased risk for patients after trans­plan­tation.


Lateral Flow Readout for CRISPR/​Cas-​​based detection strategies

Test– and Control-​​line of the HybriDetect

SHERLOCK and DETECTR are combi­na­tions of isothermal ampli­fi­cation and sensitive CRISPR/​Cas-​​based amplicon recog­nition. The compat­i­bility with the rapid, simple and equipment-​​free HybriDetect lateral flow platform is an essential feature of these methods. Inter­pre­tation of the lateral flow strips is very intuitive and easy.


Diagnostic CRISPR Tools and Techniques

Lateral Flow Assays using Genome Editing Tools

What is CRISPR/​Cas?

The CRISPR /​ Cas system acts as an immune system equiv­alent that is anchored in the genome of bacteria and archaea. The under­lying mechanism protects the organism from phage infec­tions by sequence specific destruction of “unknown” nucleic acids. The CRISPR/​Cas-​​system has the ability to learn, remember and adapt. (1, 2)


6 new Articles, referring to the use of CRISPR/​Cas and HybriDetect

DNA and RNA Detection with the Help of HybriDetect

New techniques have been developed for the specific detection of DNA or RNA sequences. They all have in common, that they use CRISPR/​Cas12 or Cas13. The enzyme recog­nizes a specific target sequence. After binding to this sequence, its endonu­clease activity is activated and it cleaves any reporter sequence. This technique can be easily combined with our Lateral Flow Assay HybriDetect for the visual­ization of the results.


HybriDetect and Temper­ature Effects

Using higher Temper­a­tures: Part of the NAFLIA Toolbox

Higher Temper­a­tures: Stability aspects and assay Parameters

The general robustness is a charac­ter­istic feature of lateral flow devices (LFD’s). Even during longer trans­ports and extreme weather condi­tions LFD’s remain stable. Temper­ature is an important factor when it comes to robustness. Therefore our HybriDetect test strips are regularly tested at higher temper­a­tures.

Especially in the devel­opment of NALFIA’s (Nucleic Acid Lateral Flow Immunoassays), the temper­ature can influence the test result signif­i­cantly. Higher temper­a­tures can also be under­stood as tool in the assay procedure to achieve certain results. The hybridization of amplifi­cates is classified as partic­u­larly inter­esting. The use of a defined temper­ature is a crucial parameter to maintain specific hybridizing condi­tions.


Our new Website is online!

We are happy to announce the launch of our new Website. We have a new Design, new Content and a new Shop.

The Shop is directly included in our product sites to simplify your ordering process.

New Content to our specific products has been created and the structure on the product pages is improved.

Please visit www​.milenia​-biotec​.de to see all the smaller but impactful changes!

We hope you enjoy our new, more user-​​friendly Website!

If you have any Feedback, please let us know on LinkedIn or by E-​​Mail.


Detecting Viruses

Major Pathogens in Aquaculture

Several inves­tigative techniques have been developed to detect viruses causing severe fish and shrimp diseases. The methods used are LAMP and RPA combined with our Lateral Flow Dipstick (LFD) for DNA Detection: HybriDetect. The addressed viruses cause diseases with a high mortality rate and therefore have a huge economic impact in fish farming indus­tries (1 – 6).


Hygiene monitoring in breweries

Results in just 2 hours – Immediate corrective actions possible!

The quality of beer is controlled in breweries at each individual level of the production process. The presence of beer spoilage microor­ganisms is associated with an elevated risk to beer quality. Microbial conta­m­i­na­tions create a negative impact on the taste, the texture and the smell of the final product. Conta­m­i­nation can occur over the entire production process, although the area of the filling machine is the main spot for conta­m­i­nation. For this reason, filling machines are cleaned inten­sively on a regular basis and the success of the cleaning process is controlled via close hygiene monitoring.