Lateral Flow Test and Development Blog
11/03
2020
Loop mediated isothermal Amplification and Lateral Flow
Tips and Tricks for a successful Assay Development
Isothermal Amplification of Nucleic Acids — A Brief Introduction
Isothermal amplification techniques have developed massively over the past decade. Point-of-Care (POC) applications in particular benefit from the advantages of these methods. As the name suggests, DNA synthesis takes place at constant temperature if isothermal amplification methods are applied. As a result, complex and expensive equipment such as thermal cyclers are not necessary for isothermal amplification reactions. A simple incubator, heating block, water bath, suit vide cooker, the armpit or the closed hand are sufficient to carry out the amplification reaction. read more…
10/26
2020
Lateral Flow Test Development for COVID-19 based on Nucleic Acid Detection
Amplicon detection via the lateral flow test Milenia “HybriDetect”
Recently Milenia Biotec GmbH received several customer inquiries related to COVID-19 detection, due to the fact, that HybriDetect may be used for the detection of RPA, LAMP or PCR amplicons. Following up these inquiries, we did a literature search on the SHERLOCK (Specific High Sensitivity Enzymatic Reporter UnLOCKing) technique, which is a powerful tool to detect genetic information coming from viruses using CRISPR. read more…
10/05
2020
Feluda Test – A new rapid test method for the identification of SARS-CoV-2
CRISPR-test gets approval in India
Just recently, the TATA Group received approval for the commercial launch of “Feluda” (acronym for FNCas9 Editor Limited Uniform Detection Assay) by the Drugs Controller Gerneral of India.
What is Feluda?
The Feluda test is the first CRISPR/Cas9 (Clustered Regularly Interspaced Short Palindromic Repeats) rapid test available for COVID-19 detection. With this new qPCR independent point-of-care diagnostic approach, the genomic sequence of SARS-CoV-2 can be detected by Milenias lateral flow assay HybriDetect. read more…
09/25
2020
How to overcome contamination in DNA amplification based Lateral Flow Assays
Contamination can happen, no matter what type of amplification method used: LAMP, RPA or PCR
Milenias HybriDetect is a ready-to-use, universal dipstick based on the lateral flow technology for the detection of various molecules. It is mainly used for the detection of amplicons, generated by LAMP, RPA or PCR by our customers.
As in standard PCR experiments, which are analyzed by gel electrophoresis or qPCR experiments, cross-contamination is a problem our customers are faced with. Therefore we want to give some information and advice to help you avoid contaminations in RPA, LAMP or PCR assays combined with our Lateral Flow tests HybriDetect and HybriDetect 2T. read more…
09/16
2020
Milenia HybriDetect Citation List Update July 2020: What are the figures behind the list?
The use of Milenia HybriDetect is described in 129 scientific publications – However, there are a lot of interesting details behind this number
In 2006 Piepenburg et al. published a paper with the title “DNA detection using recombination Proteins” in PLoS, describing the invention of the RPA technology. It was also the very first paper citing the use of Milenia HybriDetect. read more…
08/31
2020
A customers question: What is the limit of detection of Milenia HybriDetect?
Milenia HybriDetect is a tool for our customers and us to develop lateral flow based point of care tests.
Test Principle
The principle works as such that biotin and FITC and – if applicable – digoxigenin and FITC labeled compounds are introduced into the test mixture. These compounds form complexes, which are finally detected by the universal lateral flow strips, Milenia HybriDetect.
Since the market introduction in the year 2003 a high number of immunoassays and molecular biology method based tests have been developed by researchers. At the time of writing this article in July 2020 we could find 129 scientific papers citing the use of Milenia HybriDetect. read more…
08/21
2020
A Tool for Mouse Monoclonal Antibody Isotype, Subtype and light Chain Detection
The Milenia Quick Line Mouse Isotyping Kit is designed for fast and easy identification of mouse antibody isotypes, subtypes and light chains and offers numerous applications
Hybridoma Technology
Specific mouse monoclonal antibodies can be produced by the Hybridoma Technology. This method results in immortal hybrid cells formed by B-lymphocytes and myeloma cells. After the main steps (immunization of the host animal, B-cell and myeloma cell fusion) the experimenter needs to know which subclass is produced by the cells in order to select an antibody clone with the desired features. read more…
05/15
2020
Improving the sensitivity of the HybriDetect 2T
How to use block oligos for better results
Detecting various PCR amplificates with HybriDetect 2T
The amplification of nucleic acids has become an important tool for various areas such as medical diagnostics, food safety and research over the past decades. A quick and easy way to get a visible result of the amplification is to evaluate the amplification product using the HybriDetect 2T strip, a lateral flow evaluation strip.
read more…
05/07
2020
Lateral Flow based post-transplantation Monitoring
CRISPR-based diagnosis of infection and rejection with the HybriDetect
HybriDetect helps diagnose viral infections and post-transplantation Monitoring with SHERLOCK
Kaminski et al. developed a fast and easy test to detect causes of graft loss in blood and urine samples from post-transplant patients after kidney transplantation.
The need for a new method to detect organ rejections is highly given, because current invasive methods can take several days and are connected with high costs. The loss of time can lead to an increased risk for patients after transplantation.
read more…
04/30
2020
Lateral Flow Readout for CRISPR/Cas-based detection strategies
Test– and Control-line of the HybriDetect
SHERLOCK and DETECTR are combinations of isothermal amplification and sensitive CRISPR/Cas-based amplicon recognition. The compatibility with the rapid, simple and equipment-free HybriDetect lateral flow platform is an essential feature of these methods. Interpretation of the lateral flow strips is very intuitive and easy.
read more…