Taenia solium IgG

Cysticer­cosis is caused by larval cysts of the tapeworm Taenia solium and is a major cause of adult onset seizures in most devel­oping countries. Diagnosis is based on imaging findings, but in many cases it is not conclusive for the diagnosis. Serological assays has an important place on the final diagnosis of the disease. The ELISA kit hs shown appro­priate perfor­mances for the serological screening of cysticer­cosis.

Name of Product
Taenia solium IgG
Catalog Number
AF 9700
Short Info

Cysticer­cosis is caused by larval cysts of the tapeworm Taenia solium and is a major cause of adult onset seizures in most devel­oping countries. Diagnosis is based on imaging findings, but in many cases it is not conclusive for the diagnosis. Serological assays has an important place on the final diagnosis of the disease. The ELISA kit hs shown appro­priate perfor­mances for the serological screening of cysticer­cosis.

Method/​Patform
ELISA in microplate format
Range/​Assay Sensivity
98% sensi­tivity, 98% speci­ficity
Test Principle

The kit provides all the material needed to perform 96 enzyme-​​linked immunosorbent assays (ELISA) on breakable microti­tration wells sensi­tized with Taenia solium cyst soluble antigens. Specific antibodies in the sample will bind to these antigens and washing will remove unspe­cific antibodies. The presence of parasite specific antibodies is detected with a Protein A – alkaline phosphatase conjugate. A second washing step will remove unbound conjugate. Revealing bound antibodies is made by the addition of pNPP substrate which turns yellow in the presence of alkaline phosphatase. Color intensity is propor­tional to the amount of Taenia solium specific antibodies in the sample. Potassium phosphate is added to stop the reaction. Absorbance at 405 nm is read using an ELISA microplate reader. The test can be performed with automatic systems, but this must be validated by the user.

Brief Instruc­tions

Step 1: Blocking 5 – 15 min at room temper­ature

Step 2: Incubation with samples 30 min at 37°C

Step 3: Incubation with conjugate 30 min at 37°C

Step 4: Incubation with substrate 30 min at 37°C

Step 5: Measurement of absorbences

Storage
2 – 8° C
Compo­nents
ELISA wells, dilution buffer, washing solution, control sera, conjugate, substrate solution, stopping solution

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