Fasciola hepatica IgG

Fasci­olosis is one of the most frequently encoun­tered autochthonous helminthic infec­tions in Central Europe. The immun­odi­ag­nosis of Fasciola hepatica infec­tions is challenged by high serological cross-​​reacitivity with other (hepatic) parasito­logical infec­tions encoun­tered in Central Europe, such as alveolar echinococ­cosis, toxocarosis and ascar­iosis, but also other parasitic infec­tions acquired during overseas travel. The SAP-​​2 recom­binant antigen shows a high speci­ficity, especially with patients with other parasitic infec­tions. It is a suitable serological test for routine diagnosis of human fasci­olosis, partic­u­larly if the results are supported by clinical history.

Name of Product
Fasciola hepatica IgG
Catalog Number
AF 9650
Short Info

Fasci­olosis is one of the most frequently encoun­tered autochthonous helminthic infec­tions in Central Europe. The immun­odi­ag­nosis of Fasciola hepatica infec­tions is challenged by high serological cross-​​reacitivity with other (hepatic) parasito­logical infec­tions encoun­tered in Central Europe, such as alveolar echinococ­cosis, toxocarosis and ascar­iosis, but also other parasitic infec­tions acquired during overseas travel. The SAP-​​2 recom­binant antigen shows a high speci­ficity, especially with patients with other parasitic infec­tions. It is a suitable serological test for routine diagnosis of human fasci­olosis, partic­u­larly if the results are supported by clinical history.

Method/​Patform
ELISA in microplate format
Range/​Assay Sensivity
77% sensi­tivity, 98% speci­ficity
Test Principle

The kit provides all the material needed to perform 96 enzyme-​​linked immunosorbant assays (ELISA) on breakable microti­tration wells sensi­tized with Fasciola hepatica recom­binant antigen. Specific antibodies in the sample will bind to the antigen and washing will remove unspe­cific antibodies. The presence of parasite specific antibodies is detected with a Protein A – alkaline phosphatase conjugate. A second washing step will remove unbound conjugate. Revealing bound antibodies is made by the addition of pNPP substrate which turns yellow in the presence of alkaline phosphatase. Color intensity is propor­tional to the amount of Fasciola hepatica specific antibodies in the sample. Potassium phosphate is added to stop the reaction. Absorbance at 405 nm is read using an ELISA microplate reader.The test can be performed with automatic systems, but this must be validated by the user.

Brief Instruc­tions

Step 1: Blocking 5 – 15 min at room temper­ature

Step 2: Incubation with samples 30 min at 37°C

Step 3: Incubation with conjugate 30 min at 37°C

Step 4: Incubation with substrate 30 min at 37°C

Step 5: Measurement of absorbences

Storage
2 – 8° C
Compo­nents
ELISA wells, dilution buffer, washing solution, control sera, conjugate, substrate solution, stopping solution

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