

Microsporidia
Indirect immunofluorescence assay for the specific diagnostic of human intestinal microsporidiosis.
This product is only available in Germany!
Indirect immunofluorescence assay for the specific diagnostic of human intestinal microsporidiosis.
This product is only available in Germany!
Monoclonal antibodies bind specifically to samples with spores (E. bienusi and E. intestinalis) attached to the slide wells. Unfixed antibodies will be washed away. The presence of spore-specific antibodies is detected with a fluorescent anti-mouse IgG conjugate.


– Filter through 50 µm (best) or 100 µm filter.
– Centrifuge at 700g for 15 minutes, discard the supernatant and resuspend the pellet (dilution 3 times) with PBS.
– Put 2 µl of the fecal sample suspension to be tested on well slides and dry for one hour. Count 2 wells per sample: 1 for each monoclonal antibody.
– Fix the slides with methanol, let dry 5 minutes.
– Add 20 µl of either of the monoclonal antibodies on the wells and incubate for 30 min at room temperature in a humid atmosphere.
– Wash 3 times with a drop of PBS, aspirating the PBS on the side of the wells without touching the sample.
– Add 20 µl of conjugate to each well. Incubate for 30 min at room temperature in a humid atmosphere, in the dark.
– Aspirate the conjugate, wash with a drop of PBS as specified above. Dip the slides in 3 changes of PBS.
– Drain the buffer from the slides. Dry the slides underneath and carefully around the samples, without touching the sample.
– Add 2 drops of anti-fading fluorescence mounting medium, place a coverslip on the wells, avoiding the formation of air bubbles.
– Reading is made with a fluorescence microscope, equiped with the appropriate fluoresceine filter (488 nm) and an immersion objective (x 1000).
2-8°C
Monoclonal antibody anti- E. bieneusi, monoclonal antibody anti- E. intestinalis, conjugate
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Simple species diagnosis of human intestinal microsporidia by an immunofluorescence test using specific monoclonal antibodies : an evaluation study in two hospitals in France. (2004)
M. Thellier, I. Accoceberry , I. Desportes, S. Biligui, E. Bart-Delabesse, C. Ripert, M. Danis, A. Datry