Microsporidia

Indirect immuno­flu­o­res­cence assay for the specific diagnostic of human intestinal microsporidiosis.

This product is only available in Germany!

Name of Product
Microsporidia
Catalog Number
AF 8100
Short Info

Indirect immuno­flu­o­res­cence assay for the specific diagnostic of human intestinal microsporidiosis.

This product is only available in Germany!

Method/​Patform
Indirect immuno­flu­o­res­cence test
Range/​Assay Sensivity
Test Principle

Monoclonal antibodies bind specif­i­cally to samples with spores (E. bienusi and E. intesti­nalis) attached to the slide wells. Unfixed antibodies will be washed away. The presence of spore-​​specific antibodies is detected with a fluorescent anti-​​mouse IgG conjugate.

Brief Instruc­tions

– Filter through 50 µm (best) or 100 µm filter.

– Centrifuge at 700g for 15 minutes, discard the super­natant and resuspend the pellet (dilution 3 times) with PBS.

– Put 2 µl of the fecal sample suspension to be tested on well slides and dry for one hour. Count 2 wells per sample: 1 for each monoclonal antibody.

– Fix the slides with methanol, let dry 5 minutes.

– Add 20 µl of either of the monoclonal antibodies on the wells and incubate for 30 min at room temper­ature in a humid atmos­phere.

– Wash 3 times with a drop of PBS, aspirating the PBS on the side of the wells without touching the sample.

– Add 20 µl of conjugate to each well. Incubate for 30 min at room temper­ature in a humid atmos­phere, in the dark.

– Aspirate the conjugate, wash with a drop of PBS as specified above. Dip the slides in 3 changes of PBS.

– Drain the buffer from the slides. Dry the slides under­neath and carefully around the samples, without touching the sample.

– Add 2 drops of anti-​​fading fluores­cence mounting medium, place a coverslip on the wells, avoiding the formation of air bubbles.

– Reading is made with a fluores­cence micro­scope, equiped with the appro­priate fluores­ceine filter (488 nm) and an immersion objective (x 1000).

Storage
2 – 8°C
Compo­nents
Monoclonal antibody anti– E. bieneusi, monoclonal antibody anti– E. intesti­nalis, conjugate