Leishmania infantum - IgG ELISA

Name: Leishmania infantum
Brand: Milenia Biotec GmbH
SKU: This ELISA kit is
Price: 559 EUR
Availability: InStock

559.00 €

(Price excludes VAT and shipping costs)

12 x 8 (96 tests)

This ELISA kit is intended for the quantitative detection of IgG antibodies against Leishmania infantum in human serum.

This product is only available in Germany!

Overview

Name of Product

Leishmania infantum - IgG ELISA

Catalog Number

AF 9500

Short Info

This ELISA kit is intended for the quantitative detection of IgG antibodies against Leishmania infantum in human serum.

This product is only available in Germany!

Method/Platform

ELISA in microplate format

Range/Assay Sensivity

pNPP, λ=405 nm

Test Principle

Specific antibodies in the sample bind to Leishmania infantum antigens sensitized on microtiter plates. The presence of parasite specific antibodies is detected with a Protein A – alkaline phosphatase conjugate.

Documents

Additional information (external link)

Protocol

Brief Instructions

Step 1: Blocking 5-15 min at room temperature

Step 2: Incubation with samples 30 min at 37°C

Step 3: Incubation with conjugate 30 min at 37°C

Step 4: Incubation with substrate 30 min at 37°C

Step 5: Measurement of absorbences

Storage

2-8°C

Components

ELISA wells, dilution buffer, washing solution, control sera, conjugate, substrate solution, stopping solution

References

Evaluation of the micro enzyme-linked immunosorbent assay (ELISA) for antibodies in american visceral leishmaniasis : antigen selection for detection of infection specific responses. Am. J. Trop. Med. Hyg. 35: 72-78. (1996)
Badaro, R., Reed, S.G., Barral, A., Orge, G., Jones, T.C.
Experimental canine leishmaniasis: Clinical, parasitological and serological follow-up. Acta Tropica. 116: 193-199. (2010)
Maia, C., Nunes, M., Cristovao, J., Campino, L.
Leishmaniose viscérale au cours de l’infection à VIH : avantages du sérodiagnotic par technique ELISA. Pres. Med. 23 : 672-673.
Daleine, G., Deniau, M., Matheron, S., Leport, C., Lebras, J.
Leishmaniose viscérale au cours du sida : Difficultés du diagnostic sérologique, évaluation d’une nouvelle trousse ELISA. Feuillets de Biologie. 37: 39-42.
Nassar, N., Gangneux, J.P., Sulahian, A., Derouin, F.
Serological diagnosis of visceral leishmaniasis by a dot-enzyme immunoassay for the detection of a Leishmania donovani-related circulating antigen. Journal of Immunological Methods. 193: 9-15. (1996)
Senaldi, G., Xiao-su, H., Hoessli, D.C., Bordier, C.

Leishmania infantum - IgG ELISA

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