Strongyloides ratti - IgG ELISA

Name: Strongyloides ratti
Brand: Milenia Biotec GmbH
SKU: This ELISA kit is
Price: 559 EUR
Availability: InStock

559.00 €

(Price excludes VAT and shipping costs)

12 x 8 strips (96 test)

This ELISA kit is intended for the quantitative detection of IgG antibodies against Strongyloides nematodes in human serum.

This product is only available in Germany!

Overview

Name of Product

Strongyloides ratti - IgG ELISA

Catalog Number

AF 9450

Short Info

This ELISA kit is intended for the quantitative detection of IgG antibodies against Strongyloides nematodes in human serum.

This product is only available in Germany!

Method/Platform

ELISA in microplate format

Range/Assay Sensivity

pNPP, λ=405 nm

Test Principle

Specific antibodies in the sample bind to Strongyloides nematodes antigens sensitized on microtiter plates. The presence of parasite specific antibodies is detected with a Protein A alkaline phosphatase conjugate.

Documents

Additional information (external link)

Protocol

Brief Instructions

Step 1: Blocking 5-15 min at room temperature

Step 2: Incubation with samples 30 min at 37°C

Step 3: Incubation with conjugate 30 min at 37°C

Step 4: Incubation with substrate 30 min at 37°C

Step 5: Measurement of absorbences

Storage

2-8°C

Components

ELISA wells, dilution buffer, washing solution, control sera, conjugate, substrate solution, enzyme buffer, stopping solution

References

A retrospective study of autochthonous strongyloïdiasis in Région Midi-Pyrénées (Southwesten France). Eur. J. Epidemiol. 16, 179-182. (2000)
Magnaval, J.-F., Mansuy, J.M., Villeneuve, L. and Cassaing, S.
Accuracy of Five Serologic Tests for the follow-up of Strongyloïdes stercoralis infection. PLos Negl. Trop. Dis. 9. (2015)
Buonfrate, D., Sequi, M., Mejia, R., Cimino, R.O., Kroleiecki, A., Albonico, M., et al.
Diagnostic accuracy of five serologic tests for Strongyloïdes stercoralis infection. Plos. Negl. Trop. Dis. 8. (2014)
Bisoffi, Z., Buonfrate, D., Sequi, M., Mejia, R., Cimino, R.O. et al.
Serology and eosinophil count in the diagnosis and management of strongyloïdiasis in non endemic area. Am. J. Trop. Med. Hyg. 66, 749-752. (2002)
Loutfy, M.R., Wilson, M., Keystone, J.S. and Kain, K.C.
The value of an immunoenzymatic test (ELISA) for the diagnosis of Strongyloïdiasis in patients immunosuppressed by hematologic malignancies. Am. J. Trop. Med. Hyg. 65, 346-350. (2001)
Schaffel, R., Nucci, M., Carvalho, E., Braga, M., Almeida. L., Portugal, R. and Pulcheri, W.
Use of ELISA and dipstick assay for the detection of Strongyloïdes stercoralis infection in humans. J. Clin. Microbiol. 45, 438-442. (2007)
Van Doorn, H.R., Koelewijn, R., Hofwegen, H., Gilis, H., Wetsteyn, J.C.F.M., Wismans, P.J., Sarfati, C., Vervoort, T. and Gool, T.

Strongyloides ratti - IgG ELISA

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