Toxocara canis — IgG
This ELISA kit is intended for the quantitative detection of IgG antibodies against Toxocara canis in human serum.
This product is only available in Germany!
Name of Product
Toxocara canis — IgG
Catalog Number
AF 9200
Short Info
This ELISA kit is intended for the quantitative detection of IgG antibodies against Toxocara canis in human serum.
This product is only available in Germany!
Method/Patform
ELISA in microplate format
Range/Assay Sensivity
pNPP, λ=405 nm
Test Principle
Specific antibodies in the sample bind to Toxocara canis antigens sensitized on microtiter plates. The presence of parasite specific antibodies is detected with a Protein A alkaline phosphatase conjugate.
Brief Instructions
Step 1: Blocking 5 – 15 min at room temperature
Step 2: Incubation with samples 30 min at 37°C
Step 3: Incubation with conjugate 30 min at 37°C
Step 4: Incubation with substrate 30 min at 37°C
Step 5: Measurement of absorbences
Storage
2 – 8°C
Components
ELISA wells, dilution buffer, washing solution, control sera, conjugate, substrate solution, enzyme buffer, stopping solution
- Choi, D., Lim, J.H., Choi D-C., Paik, S-W. and Huh, S. (2008) Toxocariasis and Ingestion of Raw Cow Liver in Patients with Eosinophilia. Korean J. Parasitol. 46, 139 – 143.
- De Savigny, D.H., Voller, A. and Woodruff, A.W. (1979) Toxocariasis: serological diagnosis by enzyme immunoassay. J. Clin. Path. 32 : 284 – 288.
- Jacquier, P., Gottstein, B., Singelin, Y. and Eckert, J. (1991) Immunodiagnosis of Toxocariasis in Human: Evaluation of a New Enzyme-Linked Immunosorbent Asay Kit. J. Clin. Microbiol. 29, 1831 – 1835.
- Jin, Y., Shen, C., Huh, S., Sohn, W-M., Choi, M-H. and Hong, S-T. (2013) Serodiagnosis of Toxocariasis by ELISA Using Crude Antigen of Toxocara canis Larvae. Korean J. Parasitol. 51, 433 – 439.
- Speiser, F. and Gottstein, B. (1984) A collaborative study on larval excretory/secretory antigens of Toxocara canis for the immunodiagnosis of human toxocariasis with ELISA. Acta Tropica. 41, 361 – 372.