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Milenia HybriDetect allows point-of-care test development for anyone

With Milenia HybriDetect a point-of-care test strip is available, serving users as a platform for own test developments, regardless whether these are immunoassays or molecular biology detection systems

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7 important reasons why you should use Milenia HybriDetect

1. Multiple test devel­opment appli­ca­tions

You can use Milenia HybriDetect for point-​​of-​​care test devel­opment projects in many different appli­ca­tions. The product is frequently used for the devlopment of molecular biology methods. So far the detection of products derived from ampli­fi­cation methods, including RPA, LAMP and PCR, are frequently described. However, molecular biology detection methods without an ampli­fi­cation step are also possible to develop with Milenia HybriDetect. One example is the detection of ribosomal RNA (rRNA) via a sandwich hybridization. The devel­opment of immunoassays is also possible with HybriDetect. Whether this is a sandwichim­munoassay or a compet­itive test, no problem at all with Milenia HybriDetect!
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2. Speed

After applying the sample to the Milenia HybriDetect test strip highly positive samples can be identified within seconds. Usually results can be reported within 5 – 30 minutes, depending on the appli­cation of the product.
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3. Sensi­tivity

Milenia HybriDetect is 100 – 1.000 x more sensitive than an agarose gel when molecular biology products are detected. Agarose gels are the basic method used by researchers all over the world for the detection of molecular biology products.
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4. Cost effec­tiveness

The results of Milenia HybriDetect are visible to the naked eye. Therfore no equipment is necessary to report results. After performing the tests users can fix the HybriDetect strips on a report sheet, that can be stored in a binder or scanned or an image can be taken.
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5. Point-​​of-​​need appli­ca­tions

Due to simple handling, inter­pre­tation and documen­tation, the use of Milenia HybriDetect is not bound to a laboratory and can therefore be used at any place, whether this is a field, a pond or any other location!
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6. Frequently cited in the scien­tific liter­ature

Since the release of HybriDetect to the market in 2003 the product has been used by many researchers and is cited in a series of high quality publi­ca­tions. A list of all these publi­ca­tions is available here.
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7. Reliable quality, available at any time

Milenia HybriDetect is manufac­tured by Milenia Biotec GmbH, Gießen, Germany under the rules of a certified quality management system (EN ISO 13485:2016). The test strips are an integral component of a number of diagnostic kits manufac­tured by Milenia Biotec GmbH and other partners from the diagnostic industry. Up to now more than 3.000.000 HybriDetect test strips have been manufac­tured. The high demand for HybriDetect allows Milenia Biotec to keep adequate levels of kits in inventory. Even after receipt of big volume orders kits can be shipped the same day from stock. Milenia Biotec delivers daily and worldwide.
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Buy online

Buy HybriDetect online now

How to perform HybriDetect - Detection of PCR/LAMP/RPA-Amplicons

HybriDetect - Frequently asked questions

A:
Yes, but initially negative strips may show a faint band after prolonged time, while positive bands will not disappear! We recommend to take an image of the strip shortly after the incubation time is over.
A:
HybriDetect allows the detection of 1 target on the strip, while HybriDetect 2T allows the detection of 2 targets on the same strip.
A:
This depends on the size of the amplicon. The shorter the amplicon is, the lower is the sensi­tivity of the agarose gel. If the amplicon is 100 bases long HybriDetect could possibly be 1.000 fold more sensitive compared to the agarose gel.
A:
Yes, but initially negative strips may show a faint band after prolonged time, while positive bands will not disappear! We recommend to take an image of the strip shortly after the incubation time is over.
A:
Yes, we accept credit card for payment if you purchase our products online via our webshop.
A:
Yes, you can download the pdf-​​file of the MSDS for HybriDetect here and for HybriDetect 2T here
A:
The products are in inventory and we ship the same day if we receive the order prior 12.00 CET.
A:
No, kits can be shipped at ambient temper­a­tures, but should be stored at 2 – 8°C after receipt.
A:
Yes, the kits will still perform well. We have made stability tests and could demon­strate that the kits still perform well after storing them for 100 days at 37°C.
A:
The sum of the volume of the sample and the buffer should not exceed 100 µl.
A:
This phenomenon is due to the formation of primer dimers. In this event biotiny­lated and FITC-​​labeled primers bind to each other and form dimers resulting in false positive results. You need to redesign your primers in order to prevent primer dimer­ization.
A:
The shelf life of the kits from the time of production is 24 months. Milenia Biotec is manufac­turing fresh lots twice a year, meaning that our customers usually get kits with a remaining shelf life of not less than 12 months.
A:
Yes, in case the concen­tration of labeled primers or probes is too high, this may lead to a decreased sensi­tivity of the test. In this case primer or probe concen­tration need to be reduced. Furthermore, compo­nents of the sample or the sample buffer, including SDS, DTT or urea may have a negative impact on the perfor­mance of the test. In this case the sample can be diluted in order to „dilute the effect out“. However this approach will result in a reduced sensi­tivity of the test. It is also possible to develop a modified assay buffer to reduce the inter­fer­ences.
A:
Yes, we demon­strated that HybriDetect (MGHD 1) still performs well at temper­a­tures up to 75°C and HybriDetect 2T at temper­a­tures up to 65°C.

Publi­ca­tions

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A new method for the rapid detection of Atlantic cod (Gadus morhua), Pacific cod (Gadus macro­cephalus), Alaska pollock (Gadus chalcogrammus) and ling (Molva molva) using a lateral flow dipstick assay. Food Chem. 2017; 233, 182 – 189

Authors: Taboada L. et al.
Year: 2017
Tags: PCR, Species Detection
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A Novel Test to Diagnose Canine Visceral Leish­ma­niasis at the Point of Care. Am. J. Med Hyg. 2015, 93 (5), 970 – 975

Authors: Castellanos-​​Gonzalez A et al.
Year: 2015
Tags: Veterinary, PCR, RPA
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A Paper and Plasitc Device for the Combined Isothermal Ampli­fi­cation and Lateral Flow Detection of Plasmodium DNA. Malar. J. 2015; 14, 472

Authors: Cordray MS et al.
Year: 2015
Tags: Parasites, Infec­tious Diseases, RPA
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A rapid and precise diagnostic method for detecting the pinewood nematode Bursaphe­lenchus xylophilus by loop-​​mediated isothermal ampli­fi­cation. Phytopathology, 99(12), 1365 – 1369.

Authors: Kikuchi, T., Aikawa, T., Oeda, Y., Karim, N., & Kanzaki, N.
Year: 2009
Tags: LAMP, Agriculture, Parasites
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A recom­binase polymerase ampli­fi­cation lateral flow dipstick assay for rapid detection of the quarantine citrus pathogen in China, Phytoph­thora hiber­nalis

Authors: Dai T, Hu T, Yang X, Shen D, Jiao B, Tian W, Xu Y
Year: 2019
Tags: RPA, Agriculture
Phytoph­thora hiber­nalis is causing brown rot of citrus fruit. A rapid 20 minutes recom­binase polymerase ampli­fi­cation assay has been developed targeting the Ypt1 gene. Amplicons are detected by lateral flow dipsticks, „Milenia HybriDetect“, within 5 minutes. The assay is capable to detect 0.2 ng of Phytophtora hiber­nalis genomic DNA.
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A recom­binase polymerase ampli­fi­cation lateral flow dipstick for field diagnosis of bovine leukemia virus infection and its effec­tiveness compared to iiPCR and ELISA

Authors: Tu PA, Shiu JS, Lai FY, Chen YH, Shiau JW, Pang VF, Wang PH
Year: 2018
Tags: RPA, Veterinary
The retro­virus bovine leukemia virus (BLV) causes a persistent infection resulting in reduced milk production and reduced survival rates. This leads to substantial economic losses in the dairy industry. A recom­binase polymerase ampli­fi­cation (RPA) test combined with a lateral flow dipstick (LFD), „Milenia HybriDetect“, has been developed to allow an on-​​site BLV detection. The optimal ampli­fi­cation condition for the RPA was 30 minutes at 37˚C and followed by 5 min of LFD at room temper­ature. The sensi­tivity of the assay is 400 pg of total DNA and 10 copies of plasmid DNA.
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Buy online

Buy HybriDetect online now