A CRISPR-based assay for the detection of opportunistic infections post-transplantation and for the monitoring of transplant rejection.
Authors: Kaminski M., Alcantar M., Lape I., et.al.
Year: 2020
Tags: CRISPR, Infectious Diseases, RPA
A new method for the rapid detection of Atlantic cod (Gadus morhua), Pacific cod (Gadus macrocephalus), Alaska pollock (Gadus chalcogrammus) and ling (Molva molva) using a lateral flow dipstick assay. Food Chem. 2017; 233, 182 – 189
Authors: Taboada L. et al.
Year: 2017
Tags: PCR, Species Detection
A Novel Test to Diagnose Canine Visceral Leishmaniasis at the Point of Care. Am. J. Med Hyg. 2015, 93 (5), 970 – 975
Authors: Castellanos-Gonzalez A et al.
Year: 2015
Tags: Veterinary, PCR, RPA
A Paper and Plasitc Device for the Combined Isothermal Amplification and Lateral Flow Detection of Plasmodium DNA. Malar. J. 2015; 14, 472
Authors: Cordray MS et al.
Year: 2015
Tags: Parasites, Infectious Diseases, RPA
A protocol for detection of COVID-19 using CRISPR diagnostics.
Authors: Zhang F., Abudayyeh O. O., Gootenberg J.S., Mathers L.
Year: 2020
Tags: CRISPR, RPA, Virus Detection
A protocol for rapid detection of the 2019 novel coronavirus SARS-CoV-2 using CRISPR diagnostics : SARS-CoV-2 DETECTR.
Authors: Broughton J. P., Wayne D., Fasching C. L., Singh J., Charles Y., Chen J. S.
Year: 2020
Tags: LAMP, CRISPR, Virus Detection
A rapid and precise diagnostic method for detecting the pinewood nematode Bursaphelenchus xylophilus by loop-mediated isothermal amplification. Phytopathology, 99(12), 1365 – 1369.
Authors: Kikuchi, T., Aikawa, T., Oeda, Y., Karim, N., & Kanzaki, N.
Year: 2009
Tags: LAMP, Agriculture, Parasites
A rapid, equipment-free method for detecting Phytophthora infestans in the field using a lateral flow strip-based recombinase polymerase amplification assay.
Authors: Xinyu L., Zheng Y., Zhang F.
Year: 2020
Tags: RPA, Agriculture, Pathogen Detection
A recombinase polymerase amplification lateral flow dipstick assay for rapid detection of the quarantine citrus pathogen in China, Phytophthora hibernalis
Authors: Dai T, Hu T, Yang X, Shen D, Jiao B, Tian W, Xu Y
Year: 2019
Tags: RPA, Agriculture
Phytophthora hibernalis is causing brown rot of citrus fruit. A rapid 20 minutes recombinase polymerase amplification assay has been developed targeting the Ypt1 gene. Amplicons are detected by lateral flow dipsticks, „Milenia HybriDetect“, within 5 minutes. The assay is capable to detect 0.2 ng of Phytophtora hibernalis genomic DNA.
A recombinase polymerase amplification lateral flow dipstick for field diagnosis of bovine leukemia virus infection and its effectiveness compared to iiPCR and ELISA
Authors: Tu PA, Shiu JS, Lai FY, Chen YH, Shiau JW, Pang VF, Wang PH
Year: 2018
Tags: RPA, Veterinary
The retrovirus bovine leukemia virus (BLV) causes a persistent infection resulting in reduced milk production and reduced survival rates. This leads to substantial economic losses in the dairy industry. A recombinase polymerase amplification (RPA) test combined with a lateral flow dipstick (LFD), „Milenia HybriDetect“, has been developed to allow an on-site BLV detection. The optimal amplification condition for the RPA was 30 minutes at 37˚C and followed by 5 min of LFD at room temperature. The sensitivity of the assay is 400 pg of total DNA and 10 copies of plasmid DNA.
An enhanced isothermal amplification assay for viral detection.
Authors: Qian J., Boswell S., Chidley C., et. al.
Year: 2020
Tags: Virus Detection, RPA
An enhanced isothermal amplification assay for viral detection.
Authors: Qian J. et al
Year: 2020
Tags: LAMP, Virus Detection
An Innovative Field-Applicable Molecular Test to Diagnose Cutaneous Leishmania Viannis ssp. Infections. PLoS Negl. Trop. Dis. 2016; 10 (4)
Authors: Saldarriaga O. A. et al.
Year: 2016
Tags: RPA, Infectious Diseases, Parasites
An RxLR Effector Gene and New Biomarker in A Recombinase Polymerase Amplification Assay for Rapid Detection of Phytophthora cinnamomi.
Authors: Dai T., Wang A., Yang X., Yu X., Tain W., Xu Y
Year: 2020
Tags: RPA, Agriculture, Pathogen Detection
Application of loop-mediated isothermal amplification combined with colorimetric and lateral flow dipstick visualization as the potential point-of-care testing for Corynebacterium diphtheria.
Authors: Zasada A., Wiatrzyk A., Czajka U., et. al.
Year: 2020
Tags: LAMP, Pathogen Detection
Backpack PCR: A point-of-collection diagnostic platform for the rapid detection of Brugia parasites in mosquitoes
Authors: Zaky WI, Tomaino FR, Pilotte N, Laney SJ, Williams SA
Year: 2018
Tags: Infectious Diseases, Parasites, PCR
The authors present a novel diagnostic approach for molecular xenomonitoring of filarial parasites, like Brugia malayi, in mosquitoes that uses a rapid, NaOH-based DNA extraction methodology coupled with a portable, battery powered PCR platform and „Milenia HybriDetect“ — a test strip-based DNA detection assay.
Cas12a-based on-site and rapid nucleic acid detection of african swine fever
Authors: Bai J, Lin H, Li H, Zhou Y, Liu J, Zhong G, Wu L, Jiang W, Du H, Yang J, Xie Q, Huang L
Year: 2019
Tags: CRISPR, Infectious Diseases, Pathogen Detection, RPA
An RAA-Cas12a-based system that combines recombinase aided amplification (RAA) and CRISPR/Cas12a for african swine fever virus (ASFV) has been developed. For field detection of ASFV, „Milenia HybriDetect“, a lateral-flow strip readout was introduced for the first time in the RAA-Cas12a based system and named CORDS (Cas12a based on-site and rapid detection system).
Comparative evaluation of a novel recombinase polymerase amplification-lateral flow dipstick (RPA-LFD) assay, LAMP, conventional PCR, and leaf-disc baiting methods for detection of Phytophthora sojae
Authors: Dai T, Yang X, Hu T, Jiao B, Xu Y, Zheng X, Shen D
Year: 2019
Tags: Agriculture, LAMP, PCR, RPA
Phytophthora sojae is the causal pathogen of root and stem rot and seedling damping-off of soybean. An isothermal amplification recombinase polymerase assay was developed for the detection of Phytophthora sojae within 25 minutes. RPA products are detected by a lateral flow strip, „Milenia HybriDetect“.
COVID-19 infection diagnosis: Potential impact of isothermal amplification technology to reduce community transmission of SARS-CoV-2.
Authors: James A., Alwneh J.,
Year: 2020
Tags: Virus Detection, RPA
CRISPR-based platform for carbapenemases and emerging viruses detection using Cas12a (Cpf1) effector nuclease.
Authors: Curti L., Pereyra-Bonnet F., Repizo G., et. al.
Year: 2020
Tags: CRISPR, RPA, Virus Detection
CRISPR-based surveillance for COVID-19 using genomically-comprehensive machine learning design.
Authors: Metsky H. C., Freije C. A., Kosoko-Thoroddsen T-S. F., Sabeti P.C., Myhrvold C.,
Year: 2020
Tags: CRISPR, RPA
CRISPR-Cas fluorescent cleavage assay coupled with recombinase polymerase amplification for sensitive and specific detection of Enterocytozoon hepatopenaei.
Authors: Kanitchinda S., Srisala J., Suebsing R., et. al.
Year: 2020
Tags: CRISPR, RPA
CRISPR-Cas-amplified urine biomarkers for multiplexed and portable cancer diagnostics
Authors: Hao L., Zhao R., Ngambenjawong C., et. al.
Year: 2020
Tags: CRISPR, RPA
CRISPR-Cas12-based detection of SARS-CoV-2, Nature Biotechnology
Authors: Broughton J.P., Deng X., Yu G., Fasching C.L., Servellita V., Singh J., Miao X., Streithorst J.A., Granados A., Sotomayor-Gonzales A., Zorn K., Gopez A., Hsu E., Gu W., Miller S., Pan C.Q., Guevara H., Wadford D.A., Chen J.S., Chiu C.Y.
Year: 2020
Tags: CRISPR, LAMP, Virus Detection
CRISPR-Cas12-based detection of SARS-CoV-2.
Authors: Broghton J.P., et al.
Year: 2020
Tags: CRISPR, LAMP, Virus Detection
Detection assay for HPV16 and HPV18 by loop‑mediated isothermal amplification with lateral flow dipstick tests
Authors: Kumvongpin R, Jearanaikoon P, Wilailuckana C, Sae‑Ung N, Prasongdee P, Daduang S, Wongsena M, Boonsiri P, Kiatpathomchai W, Swangvaree SS, Sandee A, Daduang J
Year: 2017
Tags: LAMP, Infectious Diseases
Cervical cancer is caused mostly by high‑risk human papillomavirus (HR‑HPV) infection. The genotypes HPV16 and HPV18 are the most prevalent in cervical cancers. The authors describe the development of a LAMP assay combined with a lateral flow dipstick, „Milenia HybriDetect“, for the detection of HPV16 and HPV18. This assay is simpkle and user‑friendly and allows a very senditive detection of the mentioned HPV genotypes.
Detection of Entamoeba histolytica by Recombinase Polymerase Amplification. Am. J. Trop. Med. 2015; 93 (3) 591 – 595
Authors: Nair G. et al.
Year: 2015
Tags: Infectious Diseases, Parasites, RPA
Detection of Helminth Ova in Wastewater Using Recombinase Polymerase Amplification Coupled to Lateral Flow Strips.
Authors: Ravindran V. B., Khallaf B., Surapaneni A., Crosbie N.D., Soni S.K., Ball A.S.
Year: 2020
Tags: RPA, Pathogen Detection
Detection of Mycobacterium tuberculosis by using loop-mediated isothermal amplification combined with a lateral flow dipstick in clinical samples. Biomed. Res. Int. 2013; 1 – 6
Authors: Kaewphinit T., Arunrut N., Kiatpathomchai W., Santiwatanakul S., Jaratsing P., Chansiri K.
Year: 2013
Tags: LAMP, Infectious Diseases
Detection of Skeletonema costatum based on loop-mediated isothermal amplification combined with lateral flow dipstick. Mol Cell Probes. 2017 Dec;36:36 – 42.
Authors: Huang H. L. et al.
Year: 2017
Tags: LAMP, Species Detection
Development and evaluation of serotype-specific recombinase polymerase amplification combined with lateral flow dipstick assays for the diagnosis of foot-and-mouth disease virus serotype A, O and Asia1
Authors: Wang H, Hou P, Zhao G, Yu L, Gao YW, He H
Year: 2018
Tags: RPA, Veterinary
Foot-and-mouth disease (FMD) caused by foot-and-mouth disease virus (FMDV) is one of the most highly infectious diseases in livestock, and leads to huge economic losses. In this paper, a series of serotype-specific reverse transcription isothermal recombinase polymerase amplification assays combined with lateral flow dipstick (RPA-LFD), „Milenia HybriDetect“, were establish to differentiate FMDV serotypes A, O or Asia 1, respectively.
Development of a duplex lateral flow dipstick test for the detection and differentiation of Listeria spp. and Listeria monocytogenes in meat products based on loop-mediated isothermal amplification
Authors: Ledloda S, Bunroddithd K, Areekit S, Santiwatanakulc S, Chansiria K
Year: 2019
Tags: Food hygiene, Infectious Diseases, LAMP
A duplex lateral flow dipstick (DLFD) test, based on „Milenia HybriDetect 2T“, combined with loop-mediated isothermal amplification (LAMP) was developed for the identification of Listeria spp. and Listeria monocytogenes. Within 45 min and with the optimized LAMP reaction times at 63 °C, the method detection limits (MDL) with reference to genomic DNA and pure culture were 900 femtograms (fg) and 20 cfu/mL, respectively.
Development of a lateral flow recombinase polymerase amplification assay for rapid and visual detection of Cryptococcus neoformans/C. gattii in cerebral spinal fluid
Authors: Ma Q, Yao J, Yuan S, Liu H, Wei N, Zhang J, Shan W
Year: 2019
Tags: RPA, Infectious Diseases
The goal of the work presented in this paper is the development of a new rapid and simple detection method of Cryptococcus neoformans and C. gattii in cerebral spinal fluid. The LF-RPA assay is capable to detect 0.64 pg of genomic DNA of C. neoformans per reaction within 10minutes and is highly specific for Cryptococcus spp.
Development of a rapid and visual detection method for Rickettsia rickettsii combining recombinase polymerase assay with lateral flow test
Authors: Qi Y, Shao Y, Rao J, Shen W, Yin Q, Li X, Chen H, Li J, Zeng W, Zheng S, Liu S, Li Y
Year: 2018
Tags: RPA, Infectious Diseases
Rickettsia rickettsii is causing Rocky Mountain spotted fever, the most severe spotted fever group (SFG) rickettsiosis. A detection method for R. rickettsii has been developed based on a combination of a isothermal recombinase polymerase amplification (RPA) and the lateral flow (LF) test, „Milenia HybriDetect“. The time to result is 30 minutes and the sensitivity of the test is 10 – 50 copies per reaction.
Development of a rapid and visual nucleotide detection method for a Chinese epidemic strain of Orientia tsutsugamushi based on recombinase polymerase amplification assay and lateral flow test
Authors: Qia Y, Yin Q, Shaob Y, Caoa M, Lia S, Chena H, Shena W, Raoa J, Lia J, Lib X, Sunc Y, Linb Y, Dengb Y, Zengb W, Zhengb S, Liub S, Lia Y
Year: 2018
Tags: Infectious Diseases, RPA
Orientia tsutsugamushi is an obligate intracellular pathogen that causes scrub typhus. The authors descrive the development of a recombinase polymerase amplification (RPA) assay combined with a lateral flow (LF) test, using „Milenia HybriDetect“, for the detection of O. tsutsugamushi. The detection limits, sensitivity, specificity, and simulative clinical performance are evaluated.
Development of a rapid PCR-nucleic acid lateral flow immunoassay (PCR-NALFIA) based on rDNA IGS sequence analysis for the detection of Macrophomina phaseolina in soil
Authors: Pecchia S, Da Lio D
Year: 2018
Tags: PCR, Agriculture
Macrophomina phaseolina is a soil– and seed-borne generalist fungal pathogen that has a global distribution and can infect more than 500 plant species. The developed ‘Nucleic Acid Lateral Flow Immunoassay’ (NALFIA) using a generic ‘Lateral Flow Device’ (LFD), „Milenia HybriDetect“, combined with PCR employing labelled primers (PCR-NALFIA), enables to circumvent the use of electrophoresis, making the diagnostic test of Macrophomia phaesolina infection more rapid and easier.
Development of a recombinase polymerase amplification combined with a lateral flow dipstick assay for rapid detection of the Mycoplasma bovis
Authors: Zhao G, Hou P, Huan Y, He C, Wang H, He H
Year: 2018
Tags: RPA, Veterinary
Mycoplasma bovis (M. bovis) is a major etiological agent of bovine mycoplasmosis worldwide. Point-of-care testing in the field is lacking. Therefore the requirement for a simple, robust field applicable test is still existing. In the paper the development of a method for specific detection of M. bovis DNA is described, which is the combination of a a isothermal recombinase polymerase amplification (RPA) with lateral flow dipstick (LFD), „Milenia HybriDetect“.
Development of a recombinase polymerase amplification lateral flow assay for the detection of active Trypanosoma evansi infections.
Authors: Li Z., Torres J., Goossens J., et. al.
Year: 2020
Tags: RPA, Pathogen Detection
Development of an Isothermal Amplification-Based Assay for Rapid Visual Detection of an Orf Virus. Virol. J. 2016; 13:46
Authors: Yang Y et al.
Year: 2016
Tags: Virus Detection, RPA
Development of Recombinase Polymerase Amplification Assays for Detection of Orienta Tsutsugamushi or Rickettsia typhi. PLoS Negl. Trop. Dis. 2015; 9 (7)
Authors: Chao CC et al.
Year: 2015
Tags: RPA, Infectious Diseases
Development of the Rapid Test Kit for the Identification of Campylobacter spp. Based on Loop-mediated Isothermal Amplification (LAMP) in Combination with a Lateral Flow Dipstick (LFD) and Gold Nano-DNA Probe (AuNPs)
Authors: Thongphueak D, Chansiri K, Sriyapai T , Areekit S, Santiwatanakul S, Wangroongsarb P
Year: 2019
Tags: LAMP, Infectious Diseases, Pathogen Detection
The authors describe the development of a test for the detection of Campylobacter spp. in meat products by using loop-mediated isothermal amplification (LAMP) combined with a lateral-flow dipstick (LFD) – „Milenia HybriDetect“. The sensitivity of the test is 360 fg/μl and it shows no cross reativity to other bacteria tested during the study.
DNA Detectin Using Recombination Proteins
Authors: Piepenburg O., Williams C.H., Stemple D.L., Armes N.A.
Year: 2006
Tags: RPA
Enhancement of trans-cleavage activity of Cas12a with engineered crRNA enables amplified nucleic acid detection.
Authors: Nguyen L., Smith B., Jain P
Year: 2020
Tags: LAMP, CRISPR
Enhancement of trans-cleavage activity of Cas12a with engineered crRNA enables amplified nucleic acid detection.
Authors: Nguyen L., Smith B., Jain P.
Year: 2020
Tags: CRISPR, LAMP
Evaluation of CRISPR/Cas12a-based DNA detection for fast pathogen diagnosis and GMO test in rice.
Authors: Zhang Y., Xie K.,
Year: 2020
Tags: LAMP, CRISPR, Food hygiene, Pathogen Detection
Genome-informed diagnostics for specific and rapid detection of Pectobacterium species using recombinase polymerase amplification coupled with a lateral flow device
Authors: Ahmed FA, Larrea-Sarmiento A, Alvarez AM, Arif M
Year: 2018
Tags: Agriculture, RPA
Pectobacterium species cause serious bacterial soft rot diseases. They cause losses worldwide on economically important fruits and vegetables, including tomato and potato. Accurate and simple methods are necessary for rapid pathogen identification and timely management of the diseases. The authors describe the development of a isothermal recombinase polymerase amplification (RPA) method combined with a lateral flow device (LFD), „Milenia HybriDetect“, for the detection of Pectobacterium sp. directly from infected plant materials with no need for DNA isolation.
Inhibiotion of Recombinase Polymerase Amplification by Background DNA: A Lateral Flow-Based Method for Enriching Target DNA. Anal. Chem. 2015 87 (3), 1963 – 1967
Authors: Rohrman B. et al.
Year: 2015
Tags: RPA, PCR
Isothermal DNA amplification combined with lateral flow dipsticks for detection of biothreat agents. Anal Biochem. 2018 Nov 1;560:60 – 66.
Authors: Zasada A. A. et al.
Year: 2018
Tags: LAMP, RPA
Isothermal Recombinase Polymerase Amplification (RPA) of Schistosoma haematobium DNA and Oligochromatographic Lateral Flow Detection. Parasit Vectors 2015; 8
Authors: Rosser et al.
Year: 2015
Tags: Parasites, Infectious Diseases, RPA
Lateral Flow Loop-Mediated Isothermal Amplification Test with Stem Primers: Detection of Cryptosporidium Species in Kenyan Children Presenting with Diarrhea. J Trop Med. 2018 Feb 26;2018:7659730
Authors: Mamba T. S. et al.
Year: 2018
Tags: Infectious Diseases, LAMP, Parasites
Loop-mediated isothermal amplification (LAMP)-based method for rapid mushroom species identification. J. Agric. Food Chem. 2013; 61 (8), 1833 – 1840.
Authors: Vaagt F et al.
Year: 2013
Tags: LAMP, Species Detection
Loop-Mediated Isothermal Amplification-Lateral-Flow Dipstick (LAMP-LFD) to detect Toxoplasma gondii oocyst in ready-to-eat salad
Authors: Lalle M, Possenti A, P. Dubey JP, Pozio E
Year: 2018
Tags: LAMP, Infectious Diseases
Toxoplasma gondii is the causative agent of toxoplasmosis, a foodborne zoonosis with a global distribution. The authors the development of a sensitive and robust method based on a LAMP assay, targeting the 529 bp locus, to detect Toxoplasma gondii oocysts. The sensitivity of the test is as low as 25 oocysts/50 g in ready-to-eat baby salad. For the detection of the LAMP amplicons a lateral flow dipstick, „Milenia HybriDetect“, has been used in order to allow point-of-need application.
Molecular method for rapid detection of the red tide dinoflagellate Karenia mikimotoi in the coastal region of Xiangshan Bay, China.
Authors: Huang H. L., Gao W.F., Zhu P., Zhou C.X., Qiao L.L., Dang C.Y., Pang J.H., Yan X.J.,
Year: 2020
Tags: LAMP, Pathogen Detection
Nucleic acid detection of plant genes using CRISPR-Cas13
Authors: Abudayyeh OO, Gootenberg JS, Kellner MJ, Zhang F
Year: 2019
Tags: Agriculture, CRISPR, RPA
The authors descibe a modified version of the CRISPR-based nucleic acid detection platform SHERLOCK to quantify levels of a glyphosate resistance gene in a mixture of soybeans and to detect multiple plant genes in a single reaction. This field-applicable SHERLOCK platform with color-based lateral flow – „Milenia HybriDetect“ — readout can be applied for detection and quantitation of genes in many agricultural applications.
Nucleic Acid Detection Using CRISPR/Cas Biosensing Technologies.
Authors: Aman R., Mahas A., Mahfouz M.,
Year: 2020
Tags: CRISPR, RPA
Optimization of the Isothermal Amplification Method for Mycobacterium Tuberculosis Detection and Visualization for Fieldwork.
Authors: Ağel H.E., Sağcan H., Ceyhan I., Durmaz R.
Year: 2020
Tags: LAMP, Pathogen Detection
Point-of-care testing for COVID-19 using SHERLOCK diagnostics.
Authors: Joung J., Ladha A., Saito M., et. al.
Year: 2020
Tags: CRISPR, RPA, Virus Detection
Rapid analysis of Escherichia coli O157:H7 using isothermal recombinase polymerase amplification combined with triple-labeled nucleotide probes.
Authors: Hu J., Wang Y., Su H., et. al.
Year: 2020
Tags: RPA, Pathogen Detection
Rapid and accurate detection of novel coronavirusSARS-CoV-2 using CRISPR-Cas3.
Authors: Yoshimi K., Takeshita K., Yamayoshi S., et. al.
Year: 2020
Tags: CRISPR, RPA, Virus Detection
Rapid and accurate species identification for ecological studies and monitoring using CRISPR-based SHERLOCK.
Authors: Baerwald M., Goodbla A., nagarajan R., Gootenberg J., Abudayyeh O., Zhang F., Schreier A.
Year: 2020
Tags: CRISPR, RPA, Virus Detection
Rapid and sensitive detection of Babesia bovis and Babesia bigemina by loop-mediated isothermal amplification combined with a lateral flow dipstick Vet. Parasitol 2016; 219, 71 – 76
Authors: Yang Y et al.
Year: 2016
Tags: LAMP, Parasites, Veterinary
Rapid and sensitive detection of Candidatus Liberibacter asiaticus by loop mediated isothermal amplification combined with a lateral flow dipstick BMC Microbiol. 2014; 1186
Authors: Rigano AL et al.
Year: 2014
Tags: Agriculture, LAMP
Rapid and sensitive detection of infectious spleen and kidney necrosis virus by loop-mediated isothermal amplification combined with a lateral flow dipstick
Authors: W.C. Ding, Jiong Chen, Y.H. Shi, X.J. Lu, M.Y. Li
Year: 2010
Tags: LAMP, Virus Detection
Rapid and sensitive detection of infectious spleen and kidney necrosis virus by recombinase polymerase amplification combined with lateral flow dipsticks.
Authors: Li H., Yuan G., Luo Y., Yu Y., Ai T., Su J.
Year: 2020
Tags: RPA, Virus Detection
Rapid and simple detection of Glaesserella parasuis in synovial fluid by recombinase polymerase amplification and lateral flow strip
Authors: Zhang TT, Liu MZ, Yin RH, Yao LQ, Liu BS, Chen ZL
Year: 2019
Tags: Infectious Diseases, Pathogen Detection, RPA, Veterinary
Glaesserella parasuis (G. parasuis) is a pathogen of the pig, inducing high morbidity and mortality. The authors have developed a simple recombinase polymerase amplification (RPA) test with a Lateral flow strip, „Milenia HybriDetect“ allowing the sensitive and specific detection of Glaesserella parasuis within 60 minutes.
Rapid and visual detection of enterovirus using recombinase polymerase amplification combined with lateral flow strips.
Authors: Yang X., Xie J., Hu S., Zhan W., Duan L., Chen K., Zhang C., Yin A., Luo M
Year: 2020
Tags: Virus Detection, RPA
Rapid and visual detection of porcine deltacoronavirus by recombinase polymerase amplification combined with a lateral flow dipstick
Authors: Gao X., Liu X., Zhang Y., et. al.
Year: 2020
Tags: Veterinary, RPA, Virus Detection
Rapid and visual detection of Trichinella spp. using a lateral flow strip-based recombinase polymerase amplification (LF-RPA) assay
Authors: Li TT, Wang JL, Zhang NZ, Li WH, Yan HB, Li L,Jia WZ, Fu BQ
Year: 2019
Tags: Infectious Diseases, Parasites, RPA
Trichinella spp., are amongst the most widespread parasitic nematodes. They are living primarily in the muscles of vertebrate animals and humans. A simple, rapid and accurate diagnostic assay has been developed combining recombinase polymerase amplification and a lateral flow strip (LF-RPA), called „Milenia HybriDetect“, to detect Trichinella spp. infection. The test can be performed within 25 minutes and is capable to detect less than 100 fg DNA.
Rapid and visual detection of Verticillium dahliae using recombinase polymerase amplification combined with lateral flow dipstick.
Authors: Ju Y., Li C., Shen P., et. al.,
Year: 2020
Tags: RPA, Pathogen Detection
Rapid authentication of Cordyceps by lateral flow dipstick. J. Pharm. Biomed. Anal 2015, 111, 306 – 310
Authors: Wong YL et al.
Year: 2015
Tags: PCR, Species Detection
Rapid detection and differentiation of carp oedema virus and cyprinid herpes virus-3 in koi and common carp. J Fish Dis. 2018 May;41(5):761 – 772.
Authors: Soliman H. and El-Matoubli M.
Year: 2018
Tags: RPA, Species Detection, Veterinary, Virus Detection
Rapid Detection of 2019 Novel Coronavirus SARS-CoV-2 Using a CRISPR-based DETECTR Lateral Flow Assay.
Authors: Broughton J. P., Deng X., Fasching C.L., Singh J., Streithorst J., Granados, A., Sotomayor-Gonzalez A., Zorn K., Gopez A., Hsu E., Gu W., Miller S., Pan C., Guevara H., Wadford D., Chen J., Chio C.Y.
Year: 2020
Tags: CRISPR, LAMP, Virus Detection
Rapid detection of African swine fever virus using Cas12a-based portable paper diagnostics.
Authors: Lu L., Li F., Chen Q., et. al
Year: 2020
Tags: CRISPR, RPA, Virus Detection
Rapid detection of bovine viral diarrhea virus using recombinase polymerase amplification combined with lateral flow dipstick assays in bulk milk
Authors: Hou P, Zhao G, Wang H, He C, He H
Year: 2018
Tags: RPA, Food hygiene, Veterinary
Bovine viral diarrhea virus (BVDV) is one of the most prevalent pathogens of ruminants and is economically very important. This virus can cause significant financial losses to the livestock industry worldwide. The paper describes the development of a novel isothermal recombinase polymerase amplification (RPA) methodcombined with a lateral flow dipstick (LFD), „Milenia HybriDetect“, for rapid detection of BVDV. The time to result is 20 minutes and the sensitivity is 20 copies per reaction.
Rapid detection of Burkholderia pseudomallei with a lateral flow recombinase polymerase amplification assay
Authors: Peng Y, Zheng X, Kann B, Li W, Zhang W, Jiang T, Lu J, Qin A
Year: 2019
Tags: RPA, Infectious Diseases
A novel isothermal recombinase polymerase amplification (RPA) combined with lateral flow dipstick (LF-RPA) assay, „Milenia HybriDetect“ has been developed for rapid detection of B. pseudomallei. Results can be reported within 30 minutes and the assay show a limit of detection (LOD) of 20 femtogram (fg), corresponding to approximately 26 copies of B. pseudomallei genomic DNA. Not specific equipment is needed to run the test.
Rapid diagnosis of two marine viruses, red sea bream iridovirus and viral hemorrhagic septicemia virus by PCR combined with lateral flow assay.
Authors: Seo H., Kil E., Fadhila C., et. al.
Year: 2020
Tags: PCR, Virus Detection
Rapid SARS-CoV-2 testing in primary material based on a novel multiplex LAMP assay
Authors: Schermer B., Fabretti F., Damagnez M., et. al.
Year: 2020
Tags: LAMP, Virus Detection
Rapid visual detection of cyprinid herpesvirus 2 by recombinase polymerase amplification combined with a lateral flow dipstick
Authors: Wang H, Sun M, Xu D, Podok P, Xie J, Jiang Y, Lu L
Year: 2018
Tags: RPA, Veterinary
Herpesviral haematopoietic necrosis (HVHN), caused by cyprinid herpesvirus 2 (CyHV-2), causes significant losses in crucian carp (Carassius carassius) aquaculture. Rapid and convenient DNA assay detection of CyHV-2 is useful for field diagnosis. A detection method for CyHV-2 has been developed based on a combination of a isothermal recombinase polymerase amplification (RPA) and the lateral flow (LF) test, „Milenia HybriDetect“.
Rapid visual detection of Mycobacterium avium subsp. paratuberculosis by recombinase polymerase amplification combined with a lateral flow dipstick
Authors: Zhao G, Wang H, Hou P, He C, He H
Year: 2018
Tags: RPA, Infectious Diseases, Veterinary
Paratuberculosis, also called Johne’s disease, is a chronic debilitating disease of domestic and wild ruminants. Extensive point-of-care testing is infrequent due to the lack of a robust method. The isothermal recombinase polymerase amplification (RPA) technique has applied for rapid diagnosis. The paper describes the development of a isothermal recombinase polymerase amplification method(RPA) combined with a lateral flow dipstick (LFD), „Milenia HybriDetect“, assay for the detection of DNA from Mycobacterium avium subsp. paratuberculosis.
Rapid, CRISPR-based, field-deployable detection of white spot syndrome virus in shrimp
Authors: Sullivan TJ, Arun KD, Cruz-Flores R, Bodnar AG
Year: 2019
Tags: RPA, Agriculture, CRISPR
A CRISPR-based SHERLOCK method has been developed to allow a rapid, accurate, single copy detection of White Spot Syndrome Virus, the most devastating virus which shows a major impact on global shrimp aquaculture. The combination of a paper matrix nucleic acid extraction and a lateral flow test strip — „Milenia HybriDetect“ — results in a field applicable, next-generation diagnostic tool for veterinary pathology and animal production.
Recombinase Polymerase Amplification and Lateral Flow Assay for Ultrasensitive Detection of Low-Density.
Authors: Lalremruata A., Nguyen T., MCCall M., et. al.
Year: 2020
Tags: RPA
Recombinase polymerase amplification assay combined with a lateral flow dipstick for rapid detection of Tetracapsuloides bryosalmonae, the causative agent of proliferative kidney disease in salmonids. Parasit Vectors. 2018 Apr 11;11(1):234.
Authors: Soliman H. et al.
Year: 2018
Tags: Parasites, RPA
Recombinase polymerase amplification-lateral flow (RPA-LF) assay combined with immunomagnetic separation for rapid visual detection of Vibrio parahaemolyticus in raw oysters.
Authors: Jiang W., Ren Y., Han X., Xue J., Shan T., Chen Z., Liu Y., Quan W.
Year: 2020
Tags: RPA, Food hygiene, Pathogen Detection
SHERLOCK: nucleic acid detection with CRISPR nucleases
Authors: Kellner MJ, Koob JG, Gootenberg JS, Abudayyeh OO, Zhang F
Year: 2019
Tags: CRISPR, RPA
A CRISPR-based diagnostic platform has been established combining nucleic acid pre-amplification with CRISPR – Cas enzymology for specific recognition of DNA or RNA sequences. A step-by-step instruction is provided for setting up SHERLOCK assays with RPA (recombinase-mediated polymerase pre-amplification) of DNA or RNA and subsequent Cas13– or Cas12-mediated detection via lateral flow readouts like „Milenia HybriDetect“ and fluorescence readouts that provide results in less than 1 hour.
Supporting Information Clustered Regularly Interspaced Short Palindromic Repeats / Cas9– Mediated Lateral Flow Nucleic Acid Assay.
Authors: Wang X., Xion E., Tian T., Cheng M., Lin W., Wang H.
Year: 2020
Tags: RPA, CRISPR
The development of highly specific and sensitive primers for the detection of potentially allergenic soybean (Glycine max) using loop-mediated isothermal amplification combined with lateral flow dipstick (LAMP-LFD).
Authors: Allgöwer S., Hartmann C., Holzhauser T.
Year: 2020
Tags: LAMP, Species Detection
The Development of Loop-Mediated Isothermal Amplification Combined with Lateral Flow Dipstick for Detection of Karlodinium veneficum. Harmful Algae 2017; 62, 20 – 29.
Authors: Huang HL et al.
Year: 2017
Tags: LAMP
Time course of detection of human male DNA from stained blood sample on various surfaces by Loop Mediated Isothermal Amplification and Polymerase Chain Reaction
Authors: Kanchanaphum P
Year: 2018
Tags: LAMP, Forensic Science, PCR
The authors describe the determination of the sex of humans from blood stains taken from different surfaces and compares the time course of detection with different molecular biology techniques. They could demonstrate that the LAMP is an effective, practical, and reliable method and that the lateral flow dipsticks, „Milenia HybriDetect“, can increase the efficiency and sensitivity of the LAMP, making it more suitable for field studies. Instrumentation, like a gel electrophoresis apparatus is not required in order to perform the test.
Trends and Innovations in Biosensors for COVID-19 Mass Testing
Authors: Santiago I.
Year: 2020
Tags: LAMP, Virus Detection
Use of a recombinase polymerase amplification commercial kit for rapid visual detection of Pasteurella multocida
Authors: Zhao G, He H, Wang H
Year: 2019
Tags: Veterinary, RPA
Pasteurella multocida is a bacterium causing bovine respiratory disease and haemorrhagic septicaemia in cattle. A recombinase polymerase amplification (RPA) method combined with a lateral flow dipstick (LFD), „Milenia HybriDetect“, has been developed for the detection of Pasteurella multocida. The test can be performed within 30 minutes and shows a detection limit of up to 120 copies per reaction.
Use of reverse transcription loop-mediated isothermal amplification combined with lateral flow dipstick for an easy and rapid detection of Jembrana disease virus. Virusdisease. 2015 Sep; 26(3):189 – 95
Authors: Kusumawati A. et al.
Year: 2015
Tags: LAMP, Virus Detection
Utilization of recombinase polymerase amplification combined with a lateral flow strip for detection of Perkinsus beihaiensis in the oyster Crassostrea hongkongensis
Authors: Wu L, Ye L, Wang Z, Cui Y, Wang J
Year: 2019
Tags: RPA, Veterinary
The protist Perkinsus causes a disease called perkiniosis and is responsible for mass mortalities of many molluscan species worldwide. An LF-RPA assay has been developed which amplifies Perkinsus beihaiensis DNA using a set of primers of 20 – 25 bp in length. The test requires an incubation at 37 °C for 25 min, results were can be read within 5 min by the naked eye on a lateral flow strip, „Milenia HybriDetect“.
Visual Detection of Canine Parvovirus Based on Loop-Mediated Isothermal Amplification Combined with Enzyme-Linked Immunosorbent Assay and with Lateral Flow Dipstick. J. Vet. Med. Sci. 2014
Authors: Sun YL et al.
Year: 2014
Tags: LAMP, Virus Detection
Visual detection of porcine reproductive and respiratory syndrome virus using CRISPR-Cas13a.
Authors: Chang Y., Deng Y., Li T.
Year: 2020
Tags: CRISPR, RPA, Virus Detection