Trichinella spiralis IgG

Trichinel­losis is caused by Trichinella spiralis, a parasitic nematode of pork, horse and wild animals. Humans can be infected by eating raw or under­cooked meat from infected animals. Most infected people do not show any symptoms. However, in some cases, symptoms appear during intestinal stage (diarrhea and abdominal pain) and muscular stage (muscle pain, fever, edema). Diagnosis is based on signs and symptoms, but in many cases it is not conclusive for the diagnosis. Serological assays have an important place on the final diagnosis of the disease. The ELISA kit has shown appro­priate perfor­mance for the serological screening of trichinel­losis.

Name of Product
Trichinella spiralis IgG
Catalog Number
9750
Short Info

Trichinel­losis is caused by Trichinella spiralis, a parasitic nematode of pork, horse and wild animals. Humans can be infected by eating raw or under­cooked meat from infected animals. Most infected people do not show any symptoms. However, in some cases, symptoms appear during intestinal stage (diarrhea and abdominal pain) and muscular stage (muscle pain, fever, edema). Diagnosis is based on signs and symptoms, but in many cases it is not conclusive for the diagnosis. Serological assays have an important place on the final diagnosis of the disease. The ELISA kit has shown appro­priate perfor­mance for the serological screening of trichinel­losis.

Method/​Patform
ELISA in microplate format
Range/​Assay Sensivity
95% sensi­tivity, 98% speci­ficity
Test Principle

The kit provides all the material needed to perform 96 enzyme-​​linked immunosorbent assays (ELISA) on breakable microti­tration wells sensi­tized with Trichinella spiralis excreted/​secreted (E/​S) larval antigens. Specific antibodies in the sample will bind to these antigens and washing will remove unspe­cific antibodies. The presence of parasite specific antibodies is detected with a Protein A – alkaline phosphatase conjugate. A second washing step will remove unbound conjugate. Revealing bound antibodies is made by the addition of pNPP substrate which turns yellow in the presence of alkaline phosphatase. Color intensity is propor­tional to the amount of Trichinella spiralis specific antibodies in the sample. Potassium phosphate is added to stop the reaction. Absorbance at 405 nm is read using an ELISA microplate reader. The test can be performed with automatic systems, but this must be validated by the user.

Brief Instruc­tions

Step 1: Blocking 5 – 15 min at room temper­ature

Step 2: Incubation with samples 30 min at 37°C

Step 3: Incubation with conjugate 30 min at 37°C

Step 4: Incubation with substrate 30 min at 37°C

Step 5: Measurement of absorbences

Storage
2 – 8° C
Compo­nents
ELISA wells, dilution buffer, washing solution, control sera, conjugate, substrate solution, stopping solution

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