Entamoeba histolytica - IgG
This ELISA kit is intended for the quantitative detection of IgG antibodies against Entamoeba histolytica in human serum.
This product is only available in Germany!
This ELISA kit is intended for the quantitative detection of IgG antibodies against Entamoeba histolytica in human serum.
This product is only available in Germany!
Specific antibodies in the sample bind to Entamoeba histolytica antigens sensitized on microtiter plates. The presence of parasite specific antibodies is detected with a Protein A – alkaline phosphatase conjugate.
Step 1: Blocking 5 – 15 min at room temperature
Step 2: Incubation with samples 30 min at 37°C
Step 3: Incubation with conjugate 30 min at 37°C
Step 4: Incubation with substrate 30 min at 37°C
Step 5: Measurement of absorbences
- Nicholls, R.S., I Restrepo, M., Duque, S., Consuelo Lopez, M. and Corredor A. (1994) Standardization and evaluation of ELISA for the serodiagnostic of amoebic liver abscess. Mem. Inst. Oswaldo Cruz, Rio de Janeiro. 89, 53 – 58.
- Visser, L.G., Verweij, J.J., Van Esbroeck, M., Edeling, W.M., Clerinx, J. and Polderman, A.M. (2006) Diagnostic methods for differentiation of E. histolytica and E. dispar in carriers : performance and clinical implications in a non-endemic setting. Int. J. Med. Microbiol. 296, 397 – 403.