03/​20
2020

HybriDetect and Temper­ature Effects

Using higher Temper­a­tures: Part of the NAFLIA Toolbox

Higher Temper­a­tures: Stability aspects and assay Parameters

The general robustness is a charac­ter­istic feature of lateral flow devices (LFD’s). Even during longer trans­ports and extreme weather condi­tions LFD’s remain stable. Temper­ature is an important factor when it comes to robustness. Therefore our HybriDetect test strips are regularly tested at higher temper­a­tures.

Especially in the devel­opment of NALFIA’s (Nucleic Acid Lateral Flow Immunoassays), the temper­ature can influence the test result signif­i­cantly. Higher temper­a­tures can also be under­stood as tool in the assay procedure to achieve certain results. The hybridization of amplifi­cates is classified as partic­u­larly inter­esting. The use of a defined temper­ature is a crucial parameter to maintain specific hybridizing condi­tions.

03/​13
2020

Devel­opment of a nucleic acid based COVID-​​19 Rapid Test

Amplicon detection via the lateral flow test Milenia “HybriDetect”

Recently Milenia Biotec GmbH received several customer inquiries related to COVID-​​19 detection, due to the fact, that HybriDetect may be used for the detection of RPA, LAMP or PCR amplicons. Following up these inquiries, we a liter­ature search on the SHERLOCK (Specific High Sensi­tivity Enzymatic Reporter UnLOCKing) technique, which is a powerful tool to detect genetic infor­mation coming from viruses using CRISPR.

What is HybriDetect?

The HybriDetect is a lateral flow dipstick (LFD), which is capable to detect different molecules, including gene ampli­fi­cation products, proteins and antibodies. A commonly used appli­cation for our test is the detection of gene ampli­fi­cation products resulting from PCR, LAMP or RPA. The under­lying genetic infor­mation can be detected regardless which kind of organism need to be detected.Finally, labeled primers must be intro­duced in the ampli­fi­cation step, so that the resulting fragments are labeled and can be detected by the HybriDetect dipstick.

Results can be reported within 5 minutes after lines become visible on the test Strip.

10/​08
2019

Our new Website is online!

We are happy to announce the launch of our new Website. We have a new Design, new Content and a new Shop.

The Shop is directly included in our product sites to simplify your ordering process.

New Content to our specific products has been created and the structure on the product pages is improved.

Please visit www​.milenia​-biotec​.de to see all the smaller but impactful changes!

We hope you enjoy our new, more user-​​friendly Website!

If you have any Feedback, please let us know on LinkedIn or by E-​​Mail.