HybriDetect and Temper­ature Effects

Using higher Temper­a­tures: Part of the NAFLIA Toolbox

Higher Temper­a­tures: Stability aspects and assay Parameters

The general robustness is a charac­ter­istic feature of lateral flow devices (LFD’s). Even during longer trans­ports and extreme weather condi­tions LFD’s remain stable. Temper­ature is an important factor when it comes to robustness. Therefore our HybriDetect test strips are regularly tested at higher temper­a­tures.

Especially in the devel­opment of NALFIA’s (Nucleic Acid Lateral Flow Immunoassays), the temper­ature can influence the test result signif­i­cantly. Higher temper­a­tures can also be under­stood as tool in the assay procedure to achieve certain results. The hybridization of amplifi­cates is classified as partic­u­larly inter­esting. The use of a defined temper­ature is a crucial parameter to maintain specific hybridizing condi­tions.


Devel­opment of a nucleic acid based COVID-​​19 Rapid Test

Amplicon detection via the lateral flow test Milenia “HybriDetect”

Recently Milenia Biotec GmbH received several customer inquiries related to COVID-​​19 detection, due to the fact, that HybriDetect may be used for the detection of RPA, LAMP or PCR amplicons. Following up these inquiries, we a liter­ature search on the SHERLOCK (Specific High Sensi­tivity Enzymatic Reporter UnLOCKing) technique, which is a powerful tool to detect genetic infor­mation coming from viruses using CRISPR.

What is HybriDetect?

The HybriDetect is a lateral flow dipstick (LFD), which is capable to detect different molecules, including gene ampli­fi­cation products, proteins and antibodies. A commonly used appli­cation for our test is the detection of gene ampli­fi­cation products resulting from PCR, LAMP or RPA. The under­lying genetic infor­mation can be detected regardless which kind of organism need to be detected.Finally, labeled primers must be intro­duced in the ampli­fi­cation step, so that the resulting fragments are labeled and can be detected by the HybriDetect dipstick.

Results can be reported within 5 minutes after lines become visible on the test Strip.


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