Lateral Flow Test and Development Blog
08/31
2020
A customers question: What is the limit of detection of Milenia HybriDetect?
Milenia HybriDetect is a tool for our customers and us to develop lateral flow based point of care tests.
Test Principle
The principle works as such that biotin and FITC and – if applicable – digoxigenin and FITC labeled compounds are introduced into the test mixture. These compounds form complexes, which are finally detected by the universal lateral flow strips, Milenia HybriDetect.
Since the market introduction in the year 2003 a high number of immunoassays and molecular biology method based tests have been developed by researchers. At the time of writing this article in July 2020 we could find 129 scientific papers citing the use of Milenia HybriDetect. read more…
08/21
2020
A Tool for Mouse Monoclonal Antibody Isotype, Subtype and light Chain Detection
The Milenia Quick Line Mouse Isotyping Kit is designed for fast and easy identification of mouse antibody isotypes, subtypes and light chains and offers numerous applications
Hybridoma Technology
Specific mouse monoclonal antibodies can be produced by the Hybridoma Technology. This method results in immortal hybrid cells formed by B-lymphocytes and myeloma cells. After the main steps (immunization of the host animal, B-cell and myeloma cell fusion) the experimenter needs to know which subclass is produced by the cells in order to select an antibody clone with the desired features. read more…
05/15
2020
Improving the sensitivity of the HybriDetect 2T
How to use block oligos for better results
Detecting various PCR amplificates with HybriDetect 2T
The amplification of nucleic acids has become an important tool for various areas such as medical diagnostics, food safety and research over the past decades. A quick and easy way to get a visible result of the amplification is to evaluate the amplification product using the HybriDetect 2T strip, a lateral flow evaluation strip.
read more…
05/07
2020
Lateral Flow based post-transplantation Monitoring
CRISPR-based diagnosis of infection and rejection with the HybriDetect
HybriDetect helps diagnose viral infections and post-transplantation Monitoring with SHERLOCK
Kaminski et al. developed a fast and easy test to detect causes of graft loss in blood and urine samples from post-transplant patients after kidney transplantation.
The need for a new method to detect organ rejections is highly given, because current invasive methods can take several days and are connected with high costs. The loss of time can lead to an increased risk for patients after transplantation.
read more…
04/30
2020
Lateral Flow Readout for CRISPR/Cas-based detection strategies
Test– and Control-line of the HybriDetect
SHERLOCK and DETECTR are combinations of isothermal amplification and sensitive CRISPR/Cas-based amplicon recognition. The compatibility with the rapid, simple and equipment-free HybriDetect lateral flow platform is an essential feature of these methods. Interpretation of the lateral flow strips is very intuitive and easy.
read more…
04/09
2020
Diagnostic CRISPR Tools and Techniques
Lateral Flow Assays using Genome Editing Tools
What is CRISPR/Cas?
The CRISPR / Cas system acts as an immune system equivalent that is anchored in the genome of bacteria and archaea. The underlying mechanism protects the organism from phage infections by sequence specific destruction of “unknown” nucleic acids. The CRISPR/Cas-system has the ability to learn, remember and adapt. (1, 2)
read more…
03/27
2020
6 new Articles, referring to the use of CRISPR/Cas and HybriDetect
DNA and RNA Detection with the Help of HybriDetect
New techniques have been developed for the specific detection of DNA or RNA sequences. They all have in common, that they use CRISPR/Cas12 or Cas13. The enzyme recognizes a specific target sequence. After binding to this sequence, its endonuclease activity is activated and it cleaves any reporter sequence. This technique can be easily combined with our Lateral Flow Assay HybriDetect for the visualization of the results.
read more…
03/20
2020
HybriDetect and Temperature Effects
Using higher Temperatures: Part of the NAFLIA Toolbox
Higher Temperatures: Stability aspects and assay Parameters
The general robustness is a characteristic feature of lateral flow devices (LFD’s). Even during longer transports and extreme weather conditions LFD’s remain stable. Temperature is an important factor when it comes to robustness. Therefore our HybriDetect test strips are regularly tested at higher temperatures.
Especially in the development of NALFIA’s (Nucleic Acid Lateral Flow Immunoassays), the temperature can influence the test result significantly. Higher temperatures can also be understood as tool in the assay procedure to achieve certain results. The hybridization of amplificates is classified as particularly interesting. The use of a defined temperature is a crucial parameter to maintain specific hybridizing conditions. read more…
10/08
2019
Our new Website is online!
We are happy to announce the launch of our new Website. We have a new Design, new Content and a new Shop.
The Shop is directly included in our product sites to simplify your ordering process.
New Content to our specific products has been created and the structure on the product pages is improved.
Please visit www.milenia-biotec.de to see all the smaller but impactful changes!
We hope you enjoy our new, more user-friendly Website!
If you have any Feedback, please let us know on LinkedIn or by E-Mail.
08/12
2019
Detecting Viruses
Major Pathogens in Aquaculture
Several investigative techniques have been developed to detect viruses causing severe fish and shrimp diseases. The methods used are LAMP and RPA combined with our Lateral Flow Dipstick (LFD) for DNA Detection: HybriDetect. The addressed viruses cause diseases with a high mortality rate and therefore have a huge economic impact in fish farming industries (1 – 6).
read more…