A customers question: What is the limit of detection of Milenia HybriDetect?

Milenia HybriDetect is a tool for our customers and us to develop lateral flow based point of care tests.

Test Principle The principle works as such that biotin and FITC and – if applicable – digox­i­genin and FITC labeled compounds are intro­duced into the test mixture. These compounds form complexes, which are finally detected by the universal lateral flow strips, Milenia HybriDetect. Since the market intro­duction in the year 2003 a high number of immunoassays and molecular biology method based tests have been developed by researchers. At the time of writing this article in July 2020 we could find 129 scien­tific papers citing the use of Milenia HybriDetect. read more…

A Tool for Mouse Monoclonal Antibody Isotype, Subtype and light Chain Detection

The Milenia Quick Line Mouse Isotyping Kit is designed for fast and easy identi­fi­cation of mouse antibody isotypes, subtypes and light chains and offers numerous appli­ca­tions

Hybridoma Technology Specific mouse monoclonal antibodies can be produced by the Hybridoma Technology. This method results in immortal hybrid cells formed by B-​​lymphocytes and myeloma cells. After the main steps (immunization of the host animal, B-​​cell and myeloma cell fusion) the exper­i­menter needs to know which subclass is produced by the cells in order to select an antibody clone with the desired features. read more…

Improving the sensi­tivity of the HybriDetect 2T

How to use block oligos for better results

Detecting various PCR amplifi­cates with HybriDetect 2T The ampli­fi­cation of nucleic acids has become an important tool for various areas such as medical diagnostics, food safety and research over the past decades. A quick and easy way to get a visible result of the ampli­fi­cation is to evaluate the ampli­fi­cation product using the HybriDetect 2T strip, a lateral flow evalu­ation strip. read more…

Lateral Flow based post-​​transplantation Monitoring

CRISPR-​​based diagnosis of infection and rejection with the HybriDetect

HybriDetect helps diagnose viral infec­tions and post-​​transplantation Monitoring with SHERLOCK Kaminski et al. developed a fast and easy test to detect causes of graft loss in blood and urine samples from post-​​transplant patients after kidney trans­plan­tation. The need for a new method to detect organ rejec­tions is highly given, because current invasive methods can take several days and are connected with high costs. The loss of time can lead to an increased risk for patients after trans­plan­tation. read more…

Lateral Flow Readout for CRISPR/​Cas-​​based detection strategies

Test– and Control-​​line of the HybriDetect

SHERLOCK and DETECTR are combi­na­tions of isothermal ampli­fi­cation and sensitive CRISPR/​Cas-​​based amplicon recog­nition. The compat­i­bility with the rapid, simple and equipment-​​free HybriDetect lateral flow platform is an essential feature of these methods. Inter­pre­tation of the lateral flow strips is very intuitive and easy. read more…

Diagnostic CRISPR Tools and Techniques

Lateral Flow Assays using Genome Editing Tools

What is CRISPR/​Cas? The CRISPR /​ Cas system acts as an immune system equiv­alent that is anchored in the genome of bacteria and archaea. The under­lying mechanism protects the organism from phage infec­tions by sequence specific destruction of “unknown” nucleic acids. The CRISPR/​Cas-​​system has the ability to learn, remember and adapt. (1, 2) read more…

6 new Articles, referring to the use of CRISPR/​Cas and HybriDetect

DNA and RNA Detection with the Help of HybriDetect

New techniques have been developed for the specific detection of DNA or RNA sequences. They all have in common, that they use CRISPR/​Cas12 or Cas13. The enzyme recog­nizes a specific target sequence. After binding to this sequence, its endonu­clease activity is activated and it cleaves any reporter sequence. This technique can be easily combined with our Lateral Flow Assay HybriDetect for the visual­ization of the results. read more…

HybriDetect and Temper­ature Effects

Using higher Temper­a­tures: Part of the NAFLIA Toolbox

Higher Temper­a­tures: Stability aspects and assay Parameters The general robustness is a charac­ter­istic feature of lateral flow devices (LFD’s). Even during longer trans­ports and extreme weather condi­tions LFD’s remain stable. Temper­ature is an important factor when it comes to robustness. Therefore our HybriDetect test strips are regularly tested at higher temper­a­tures. Especially in the devel­opment of NALFIA’s (Nucleic Acid Lateral Flow Immunoassays), the temper­ature can influence the test result signif­i­cantly. Higher temper­a­tures can also be under­stood as tool in the assay procedure to achieve certain results. The hybridization of amplifi­cates is classified as partic­u­larly inter­esting. The use of a defined temper­ature is a crucial parameter to maintain specific hybridizing condi­tions. read more…

Our new Website is online!

We are happy to announce the launch of our new Website. We have a new Design, new Content and a new Shop.

The Shop is directly included in our product sites to simplify your ordering process. New Content to our specific products has been created and the structure on the product pages is improved. Please visit www​.milenia​-biotec​.de to see all the smaller but impactful changes! We hope you enjoy our new, more user-​​friendly Website! If you have any Feedback, please let us know on LinkedIn or by E-​​Mail.

Detecting Viruses

Major Pathogens in Aquaculture

Several inves­tigative techniques have been developed to detect viruses causing severe fish and shrimp diseases. The methods used are LAMP and RPA combined with our Lateral Flow Dipstick (LFD) for DNA Detection: HybriDetect. The addressed viruses cause diseases with a high mortality rate and therefore have a huge economic impact in fish farming indus­tries (1 – 6). read more…