Diagnostic CRISPR Tools and Techniques

Lateral Flow Assays using Genome Editing Tools

What is CRISPR/​Cas? The CRISPR /​ Cas system acts as an immune system equiv­alent that is anchored in the genome of bacteria and archaea. The under­lying mechanism protects the organism from phage infec­tions by sequence specific destruction of “unknown” nucleic acids. The CRISPR/​Cas-​​system has the ability to learn, remember and adapt. (1, 2) read more…

6 new Articles, referring to the use of CRISPR/​Cas and HybriDetect

DNA and RNA Detection with the Help of HybriDetect

New techniques have been developed for the specific detection of DNA or RNA sequences. They all have in common, that they use CRISPR/​Cas12 or Cas13. The enzyme recog­nizes a specific target sequence. After binding to this sequence, its endonu­clease activity is activated and it cleaves any reporter sequence. This technique can be easily combined with our Lateral Flow Assay HybriDetect for the visual­ization of the results. read more…

HybriDetect and Temper­ature Effects

Using higher Temper­a­tures: Part of the NAFLIA Toolbox

Higher Temper­a­tures: Stability aspects and assay Parameters The general robustness is a charac­ter­istic feature of lateral flow devices (LFD’s). Even during longer trans­ports and extreme weather condi­tions LFD’s remain stable. Temper­ature is an important factor when it comes to robustness. Therefore our HybriDetect test strips are regularly tested at higher temper­a­tures. Especially in the devel­opment of NALFIA’s (Nucleic Acid Lateral Flow Immunoassays), the temper­ature can influence the test result signif­i­cantly. Higher temper­a­tures can also be under­stood as tool in the assay procedure to achieve certain results. The hybridization of amplifi­cates is classified as partic­u­larly inter­esting. The use of a defined temper­ature is a crucial parameter to maintain specific hybridizing condi­tions. read more…

Our new Website is online!

We are happy to announce the launch of our new Website. We have a new Design, new Content and a new Shop.

The Shop is directly included in our product sites to simplify your ordering process. New Content to our specific products has been created and the structure on the product pages is improved. Please visit www​.milenia​-biotec​.de to see all the smaller but impactful changes! We hope you enjoy our new, more user-​​friendly Website! If you have any Feedback, please let us know on LinkedIn or by E-​​Mail.

Detecting Viruses

Major Pathogens in Aquaculture

Several inves­tigative techniques have been developed to detect viruses causing severe fish and shrimp diseases. The methods used are LAMP and RPA combined with our Lateral Flow Dipstick (LFD) for DNA Detection: HybriDetect. The addressed viruses cause diseases with a high mortality rate and therefore have a huge economic impact in fish farming indus­tries (1 – 6). read more…

Hygiene monitoring in breweries

Results in just 2 hours – Immediate corrective actions possible!

The quality of beer is controlled in breweries at each individual level of the production process. The presence of beer spoilage microor­ganisms is associated with an elevated risk to beer quality. Microbial conta­m­i­na­tions create a negative impact on the taste, the texture and the smell of the final product. Conta­m­i­nation can occur over the entire production process, although the area of the filling machine is the main spot for conta­m­i­nation. For this reason, filling machines are cleaned inten­sively on a regular basis and the success of the cleaning process is controlled via close hygiene monitoring. read more…