Publications

The retrovirus bovine leukemia virus (BLV) causes a persistent infection resulting in reduced milk production and reduced survival rates. This leads to substantial economic losses in the dairy industry. A recombinase polymerase amplification (RPA) test combined with a lateral flow dipstick (LFD), „Milenia HybriDetect“, has been developed to allow an on-site BLV detection. The optimal amplification condition for the RPA was 30 minutes at 37˚C and followed by 5 min of LFD at room temperature. The sensitivity of the assay is 400 pg of total DNA and 10 copies of plasmid DNA.

Foot-and-mouth disease (FMD) caused by foot-and-mouth disease virus (FMDV) is one of the most highly infectious diseases in livestock, and leads to huge economic losses. In this paper, a series of serotype-specific reverse transcription isothermal recombinase polymerase amplification assays combined with lateral flow dipstick (RPA-LFD), „Milenia HybriDetect“, were establish to differentiate FMDV serotypes A, O or Asia 1, respectively.

Mycoplasma bovis (M. bovis) is a major etiological agent of bovine mycoplasmosis worldwide. Point-of-care testing in the field is lacking. Therefore the requirement for a simple, robust field applicable test is still existing. In the paper the development of a method for specific detection of M. bovis DNA is described, which is the combination of a a isothermal recombinase polymerase amplification (RPA) with lateral flow dipstick (LFD), „Milenia HybriDetect“.

Herpesviral haematopoietic necrosis (HVHN), caused by cyprinid herpesvirus 2 (CyHV-2), causes significant losses in crucian carp (Carassius carassius) aquaculture. Rapid and convenient DNA assay detection of CyHV-2 is useful for field diagnosis. A detection method for CyHV-2 has been developed based on a combination of a isothermal recombinase polymerase amplification (RPA) and the lateral flow (LF) test, „Milenia HybriDetect“.

Pasteurella multocida is a bacterium causing bovine respiratory disease and haemorrhagic septicaemia in cattle. A recombinase polymerase amplification (RPA) method combined with a lateral flow dipstick (LFD), „Milenia HybriDetect“, has been developed for the detection of Pasteurella multocida. The test can be performed within 30 minutes and shows a detection limit of up to 120 copies per reaction.

The protist Perkinsus causes a disease called perkiniosis and is responsible for mass mortalities of many molluscan species worldwide. An LF-RPA assay has been developed which amplifies Perkinsus beihaiensis DNA using a set of primers of 20–25 bp in length. The test requires an incubation at 37 °C for 25 min, results were can be read within 5 min by the naked eye on a lateral flow strip, „Milenia HybriDetect“.