03/​11/​2019

Hygiene monitoring in breweries

NEW: Hygiene monitoring in breweries: Results in just 2 hours – Immediate corrective actions possible!

Sometimes hygienic surface monitoring in the food and beverage industry can be a pain for micro­bi­o­logical quality control. Especially in breweries, the enrichment of hard-​​to-​​cultivate bacterial isolates from biofilm samples can take days to weeks. The associated results feel like a glimpse into the past. Filling the product quickly turns into a micro­bi­o­logical blind flight. Therefore, the Milenia Biotec cooperated with german brewers in order to develop a unique test, that detects obligate anaerobic beer spoiling bacteria directly from swab samples…

 

The quality of beer is controlled in breweries at each individual level of the production process. The presence of beer spoilage microor­ganisms is associated with an elevated risk to beer quality. Microbial conta­m­i­na­tions create a negative impact on the taste, the texture and the smell of the final product. Conta­m­i­nation can occur over the entire production process, although the area of the filling machine is the prime spot for secondary conta­m­i­nation. For this reason, filling machines are cleaned inten­sively on a regular basis and the success of the cleaning process is controlled via close hygienic monitoring. During this process, swabs are taken from different places on the filling machine, especially in the area of the fillers, and are trans­ferred to a selective culture medium in order to monitor the potential bacterial growth.

In the case of slow-​​growing or hard-​​to-​​cultivate bacteria being present in the swab, it can take days, up to weeks, until a positive result from a culture can be reported. In this event, initi­ation of causal cleaning activ­ities cannot be initiated.

For this reason, we are presenting a method that allows detection of beer spoilage bacteria directly from swabs within 2 hours! In this setting, the detection of obligate anaerobic bacteria of the genus Megas­phaera and Pecti­natus are of special interest. The basis of this method is the combi­nation of a rapid extraction of the bacterial nucleic acids from the swabs, followed by PCR ampli­fi­cation. Amplicons can be visualized with an easy-​​to-​​handle, rapid, universal Lateral Flow Device. Results can be inter­preted in minutes after initi­ating the Lateral Flow Analysis. The whole procedure will take approx. 2h from sample collection to result inter­pre­tation with a hands-​​on-​​time of approx. 3045 minutes.

A compar­ative study revealed that this direct detection approach showed a surpris­ingly good agreement with the culture-​​based detection of obligate beer spoiling bacteria. This technique was directly trans­ferred into practical usage by every partic­i­pating brewery!

If you have questions related to the detection of beer spoilage microor­ganisms from swabs, or you would like to receive a demon­stration in your brewery, please fill in the Contact Form and send it to us.

If you want to learn more about the Milenia GenLine Kits for the detection of beer spoiling bacteria, click here.