
HybriDetect LAMP Polymerase
The HybriDetect LAMP Polymerase kit enables the user to design and optimize his very own LAMP detection assay with high flexibility. The kit contains the following components:
- 8000 u HybriDetect LAMP Polymerase (8 u/μl)
- 2 x 1.25 ml HybriDetect LAMP Buffer (10x)
- 3 x 1.7 ml HybriDetect LAMP Enhancer (5x)
The HybriDetect LAMP Polymerase kit enables the user to design and optimize his very own LAMP detection assay with high flexibility. The kit contains the following components:
- 8000 u HybriDetect LAMP Polymerase (8 u/μl)
- 2 x 1.25 ml HybriDetect LAMP Buffer (10x)
- 3 x 1.7 ml HybriDetect LAMP Enhancer (5x)
Loop-mediated isothermal amplification (LAMP) is a nucleic acid amplification method used to rapidly amplify DNA with high specificity, efficiency, and speed under constant temperature conditions. Unlike traditional PCR, LAMP does not require thermal cycling.

Design your LAMP primers according the the general LAMP primer design rules. For evaluation with our lateral flow kits use labelled primer pairs.
- Prepare primer mix (10x) in water or TE Buffer, for example, for LAMP:
16 μM FIP, 16 μM BIP, 2 μM F3, 2 μM B3, 8 μM LoopF, 8 μM LoopB. - Prepare a 25 μl reaction
– 2.5 μl HybriDetect LAMP Buffer (10x)
– 5 μl HybriDetect LAMP Enhancer (5x)
– 2.5 μlPrimer Mix (10x)
– 1 μl Template DNA
– ad 24 μl PCR grade water
– 1.0 μl HybriDetect LAMP Polymerase (8 u/μl) - Mix gently, avoid bubbles.
- Incubate in a thermostat or qPCR instrument:
– Amplification 65°C 20-30 min
– Inactivation (optional) 80°C 10 min
Lateral Flow Evaluation
– add 2-10 μl LAMP to the test strip
– apply 40-120 μl running buffer
– incubate for 5 min
-20°C in the dark
HybriDetect LAMP Polymerase (8 u/μl) | HybriDetect LAMP Buffer (10x) | HybriDetect LAMP Enhancer (5x)