
HybriDetect LAMP Hot Start Kit
The HybriDetect LAMP Hot Start Kit enables the user to design his very own LAMP detection assay within a short time. The activity of the enzyme is blocked at ambient temperature due to molecular inhibition based hot-start technology, and the polymerase is activated only at 45 – 50°C what reduces non-specific amplification, primer dimer formation and is therefore excellent for an ambient temperature reaction setup. The kit contains the following components:
- 1.25 ml HybriDetect LAMP Hot Start Mix (2x)
- 0.125 ml HybriDetect LAMP Fluorescence Dye (20x)
- 1 ml HybriDetect PCR grade water
The HybriDetect LAMP Hot Start Kit enables the user to design his very own LAMP detection assay within a short time. The activity of the enzyme is blocked at ambient temperature due to molecular inhibition based hot-start technology, and the polymerase is activated only at 45 – 50°C what reduces non-specific amplification, primer dimer formation and is therefore excellent for an ambient temperature reaction setup. The kit contains the following components:
- 1.25 ml HybriDetect LAMP Hot Start Mix (2x)
- 0.125 ml HybriDetect LAMP Fluorescence Dye (20x)
- 1 ml HybriDetect PCR grade water
Loop-mediated isothermal amplification (LAMP) is a nucleic acid amplification method used to rapidly amplify DNA with high specificity, efficiency, and speed under constant temperature conditions. Unlike traditional PCR, LAMP does not require thermal cycling.

Design your LAMP primers according the the general LAMP primer design rules. For evaluation with our lateral flow kits use labelled primer pairs.
- Prepare primer mix (10x) in water or TE Buffer, for example, for LAMP:
16 μM FIP, 16 μM BIP, 2 μM F3, 2 μM B3, 8 μM LoopF, 8 μM LoopB. - Prepare a 25 μl reaction
– 12.5 μl HybriDetect LAMP Hot Start Mix (2x)
– 1.25 μl Optional: Fluorescence Dye (20x)
– 2.5 μl Primer Mix (10x)
– 1 μl Template DNA
– ad 25 μl PCR grade water - Mix gently, avoid bubbles.
- Incubate in a thermostat or qPCR instrument:
– Amplification 65°C 20-30 min
– Inactivation (optional) 80°C 10 min
Lateral Flow Evaluation
– add 2-10 μl LAMP to the test strip
– apply 40-120 μl running buffer
– incubate for 5 min
-20°C in the dark
HybriDetect LAMP Hot Start Mix (2x) | HybriDetect LAMP Fluorescence Dye (20x) | HybriDetect PCR grade water