Mouse Immunoglobulins Isotyping Universal Module

The Milenia QuickLine Mouse Immunoglobulins Isotyping Kit (Universal Module and individually selected Isotype specific Sample Buffers) is designed for fast and easy identification of mouse antibody isotypes, subtypes and light chains.

Only to be used in combination with freely selectable and combinable Isotype specific Sample Buffer:

MQSBG1, MQSBG2A, MQSBG2B, MQSBG2C, MQSBG3, MQSBM, MQSBA, MQSBKLC, MQSBLLC

Knowing the antibody subclass is important when mouse monoclonal antibodies are produced. Hereby the optimal purification method can be chosen for the specific isotyp which results in the highest yield of the antibody. Furthermore positive clones can easily be detected with this test after recloning, if there is little antigen available.

The Mouse Immunoglobulins Isotyping Kit is a lateral flow immunoassay designed for the determination of mouse monoclonal antibody isotypes, subtypes and light chains within 5 minutes. The immunoassay can be used for cell culture supernatants and/or purified antibodies. First the isotype specific Sample Buffer (e.g. anti-mouse IgG1) is pipetted into a well of a 96-well microtiter plate. Subsequently the test sample of unknown antibody subclass is added. If a complex is formed between the Sample Buffer and the test sample, binding to an anti-mouse antibody on the universal mouse test strip will result in a red line formation.

See our Blog to get more Information on Mouse Antibody Isotyping