Mouse-​​Isotyping Buffer IgM

The Milenia QuickLine Mouse-​​Isotyping Kit is designed for fast and easy identi­fi­cation of mouse antibody isotypes, subtypes and light chains.

The universal module (MQUM 1) and the specific Buffers (MQSBG1, MQSBG2A, MQSBG2B, MQSBG2C, MQSBG3, MQSBM, MQSBA, MQSBKLC, MQSBLLC) must be ordered separately.

Name of Product
Mouse-​​Isotyping Buffer IgM
Catalog Number
MQSBM
Short Info

The Milenia QuickLine Mouse-​​Isotyping Kit is designed for fast and easy identi­fi­cation of mouse antibody isotypes, subtypes and light chains.

The universal module (MQUM 1) and the specific Buffers (MQSBG1, MQSBG2A, MQSBG2B, MQSBG2C, MQSBG3, MQSBM, MQSBA, MQSBKLC, MQSBLLC) must be ordered separately.

Method/​Patform
immunoassay, lateral flow
Range/​Assay Sensivity
1 – 20 µg/​mL
Test Principle

Knowing the antibody-​​subclass is important when mouse monoclonal antibodies are produced. Hereby the optimal purifi­cation method can be chosen for the specific isotyp which results in the highest yield of the antibody. Furthermore positive clones can easily be detected with this test after recloning, if there is little antigen available.

The Mouse-​​Isotyping Kit is a lateral flow immunoassay designed for the deter­mi­nation of mouse monoclonal antobody isotypes, subtypes and light chains within 5 minutes. The immunoassay can be used for cell culture super­natants and/​or purified antibodies. First the isotype-​​specific Sample Buffer (e.g. anti-​​mouse IgG1) is pipetted into a well of a 96-​​well microtiter plate. Subse­quently the test sample of unknown antibody-​​subclass is added. If a complex is formed between the Sample Buffer and the test sample, binding to an anti-​​mouse antibody on the universal mouse test strip will result in a red line formation.

Brief Instruc­tions

 

 

Storage
Compo­nents