Alicy­clobacillus spp.-Screen

The Milenia® GenLine Alicy­clobacillus–Screen is designed for the quali­tative detection of Alicy­clobacillus species (ACB-​​Screen) in fruit juices.

Name of Product
Alicy­clobacillus spp.-Screen
Catalog Number
MGScACB
Short Info

The Milenia® GenLine Alicy­clobacillus–Screen is designed for the quali­tative detection of Alicy­clobacillus species (ACB-​​Screen) in fruit juices.

Method/​Patform
PCR, lateral flow
Range/​Assay Sensivity
103 — 104 cfu/​mL
Test Principle

The techno­logical basis for the GenLine tests  is the polymerase chain reaction (PCR) combined with lateral flow Tests.

Labelled specific primers are used to amplify specific DNA fragments. In addition to the target gene, a control gene, which is also present in the PCR mixes, is amplified in order to make sure that the PCR process works properly.

The resulting PCR products carry the labels of the incor­po­rated primers.

In a second part of the test, the created PCR products are detected by a lateral flow Test Strip.  A “molecular sandwich” is formed and becomes visible as a line on the test Strip.

Brief Instruc­tions
  1. The PCR reagents and the samples are prepared
  2. After the addition of the sample to the PCR reagents, the PCR is started
  3. The resulting PCR products are detected by a simple lateral flow test Strip

Storage
15°C to –20°C
Compo­nents
PCR-​​Polymerase Master-​​Mix, PCR-​​Primermix, Positive Kit-​​Control, PCR Water